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• W2756979258 abstract "Forward genetic screens using mammalian embryonic stem (ES) cells have identified genes required for numerous cellular processes. However, loss-of-function screens are more difficult to conduct in diploid cells because, in most cases, both alleles of a gene must be mutated to exhibit a phenotype. Recently, mammalian haploid ES cell lines were successfully established and applied to several recessive genetic screens. However, all these screens were performed in mixed pools of mutant cells and were mainly based on positive selection. In general, negative screening is not easy to apply to these mixed pools, although quantitative deep sequencing of mutagen insertions can help to identify some ‘missing’ mutants. Moreover, the interplay between different mutant cells in the mixed pools would interfere with the readout of the screens. Here, we developed a method for rapidly generating arrayed haploid mutant libraries in which the proportion of homozygous mutant clones can reach 85%. After screening thousands of individual mutant clones, we identified a number of novel factors required for the onset of differentiation in ES cells. A negative screen was also conducted to discover mutations conferring cells with increased sensitivity to DNA double-strand breaks induced by the drug doxorubicin. Both of these screens illustrate the value of this system." @default.
• W2756979258 created "2017-10-06" @default.
• W2756979258 creator A5044462814 @default.
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• W2756979258 date "2017-09-28" @default.
• W2756979258 modified "2023-10-16" @default.
• W2756979258 title "Arrayed mutant haploid embryonic stem cell libraries facilitate phenotype-driven genetic screens" @default.
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• W2756979258 cites W1530814332 @default.
• W2756979258 cites W1537435549 @default.
• W2756979258 cites W1664431272 @default.
• W2756979258 cites W1970069044 @default.
• W2756979258 cites W1973150820 @default.
• W2756979258 cites W1977709885 @default.
• W2756979258 cites W1981018171 @default.
• W2756979258 cites W1982521381 @default.
• W2756979258 cites W1984537581 @default.
• W2756979258 cites W1984821034 @default.
• W2756979258 cites W1985079289 @default.
• W2756979258 cites W1989459462 @default.
• W2756979258 cites W2003171404 @default.
• W2756979258 cites W2003787610 @default.
• W2756979258 cites W2004131420 @default.
• W2756979258 cites W2012891698 @default.
• W2756979258 cites W2014014132 @default.
• W2756979258 cites W2017642141 @default.
• W2756979258 cites W2018824728 @default.
• W2756979258 cites W2019845283 @default.
• W2756979258 cites W2023527508 @default.
• W2756979258 cites W2026592619 @default.
• W2756979258 cites W2027125425 @default.
• W2756979258 cites W2042673292 @default.
• W2756979258 cites W2045899482 @default.
• W2756979258 cites W2049991359 @default.
• W2756979258 cites W2051951150 @default.
• W2756979258 cites W2056231743 @default.
• W2756979258 cites W2057000572 @default.
• W2756979258 cites W2057159461 @default.
• W2756979258 cites W2057463164 @default.
• W2756979258 cites W2061445259 @default.
• W2756979258 cites W2062118486 @default.
• W2756979258 cites W2062509203 @default.
• W2756979258 cites W2064815984 @default.
• W2756979258 cites W2068212453 @default.
• W2756979258 cites W2071004446 @default.
• W2756979258 cites W2077381938 @default.
• W2756979258 cites W2081651784 @default.
• W2756979258 cites W2087746152 @default.
• W2756979258 cites W2089257968 @default.
• W2756979258 cites W2091439694 @default.
• W2756979258 cites W2097173606 @default.
• W2756979258 cites W2098717020 @default.
• W2756979258 cites W2101569447 @default.
• W2756979258 cites W2105361655 @default.
• W2756979258 cites W2108696892 @default.
• W2756979258 cites W2110841199 @default.
• W2756979258 cites W2110951885 @default.
• W2756979258 cites W2112787189 @default.
• W2756979258 cites W2118009266 @default.
• W2756979258 cites W2118967129 @default.
• W2756979258 cites W2125572458 @default.
• W2756979258 cites W2132480780 @default.
• W2756979258 cites W2133656012 @default.
• W2756979258 cites W2138035159 @default.
• W2756979258 cites W2139709723 @default.
• W2756979258 cites W2146926782 @default.
• W2756979258 cites W2149150375 @default.
• W2756979258 cites W2149236471 @default.
• W2756979258 cites W2149402050 @default.
• W2756979258 cites W2152602101 @default.
• W2756979258 cites W2158771952 @default.
• W2756979258 cites W2159074552 @default.
• W2756979258 cites W2163971374 @default.
• W2756979258 cites W2167380395 @default.
• W2756979258 cites W2171284531 @default.
• W2756979258 cites W2255998354 @default.
• W2756979258 cites W2256421302 @default.
• W2756979258 cites W2334591923 @default.
• W2756979258 cites W2339131065 @default.
• W2756979258 cites W2524450637 @default.
• W2756979258 cites W2547821618 @default.
• W2756979258 cites W2562035194 @default.
• W2756979258 cites W2575376556 @default.
• W2756979258 cites W2594752235 @default.
• W2756979258 cites W2618969857 @default.
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• W2756979258 doi "https://doi.org/10.1093/nar/gkx857" @default.
• W2756979258 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5727442" @default.
• W2756979258 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29036617" @default.
• W2756979258 hasPublicationYear "2017" @default.
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