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- W2757655825 abstract "Neutrophils use different mechanisms to cope with pathogens that invade the host organism. The most intriguing of these responses is a release of neutrophil extracellular traps (NETs) composed of decondensed chromatin and granular proteins with antimicrobial activity. An important potential target of NETs is Candida albicans-an opportunistic fungal pathogen that employs morphological and phenotype switches and biofilm formation during contact with neutrophils, accompanied by changes in epitope exposition that mask the pathogen from host recognition. These processes differ depending on infection conditions and are thus influenced by the surrounding environment. In the current study, we compared the NET release by neutrophils upon contact with purified main candidal cell surface components. We show here for the first time that in addition to the main cell wall-building polysaccharides (mannans and β-glucans), secreted aspartic proteases (Saps) trigger NETs with variable intensities. The most efficient NET-releasing response is with Sap4 and Sap6, which are known to be secreted by fungal hyphae. This involves mixed, ROS-dependent and ROS-independent signaling pathways, mainly through interactions with the CD11b receptor. In comparison, upon contact with the cell wall-bound Sap9 and Sap10, neutrophils responded via a ROS-dependent mechanism using CD16 and CD18 receptors for protease recognition. In addition to the Saps tested, the actuation of selected mediating kinases (Src, Syk, PI3K, and ERK) was also investigated. β-Glucans were found to trigger a ROS-dependent process of NET production with engagement of Dectin-1 as well as CD11b and CD18 receptors. Mannans were observed to be recognized by TLRs, CD14, and Dectin-1 receptors and triggered NET release mainly via a ROS-independent pathway. Our results thus strongly suggest that neutrophils activate NET production in response to different candidal components that are presented locally at low concentrations at the initial stages of infection. However, NET release seemed to be blocked by increasing numbers of fungal cells." @default.
- W2757655825 created "2017-10-06" @default.
- W2757655825 creator A5010290287 @default.
- W2757655825 creator A5016408905 @default.
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- W2757655825 creator A5025977756 @default.
- W2757655825 creator A5050515156 @default.
- W2757655825 creator A5055945625 @default.
- W2757655825 creator A5066036279 @default.
- W2757655825 date "2017-09-21" @default.
- W2757655825 modified "2023-10-16" @default.
- W2757655825 title "Aspartic Proteases and Major Cell Wall Components in Candida albicans Trigger the Release of Neutrophil Extracellular Traps" @default.
- W2757655825 cites W1073679039 @default.
- W2757655825 cites W1527548373 @default.
- W2757655825 cites W1533234730 @default.
- W2757655825 cites W1541195059 @default.
- W2757655825 cites W1549639459 @default.
- W2757655825 cites W1568154749 @default.
- W2757655825 cites W1571377358 @default.
- W2757655825 cites W1600560134 @default.
- W2757655825 cites W1626345020 @default.
- W2757655825 cites W1838815905 @default.
- W2757655825 cites W1881900824 @default.
- W2757655825 cites W1965897811 @default.
- W2757655825 cites W1966467200 @default.
- W2757655825 cites W1970487396 @default.
- W2757655825 cites W1988940275 @default.
- W2757655825 cites W1993800351 @default.
- W2757655825 cites W1995441274 @default.
- W2757655825 cites W1998926818 @default.
- W2757655825 cites W1999286245 @default.
- W2757655825 cites W2009571354 @default.
- W2757655825 cites W2010270225 @default.
- W2757655825 cites W2014678245 @default.
- W2757655825 cites W2014776493 @default.
- W2757655825 cites W2016385216 @default.
- W2757655825 cites W2018716686 @default.
- W2757655825 cites W2019367817 @default.
- W2757655825 cites W2019880292 @default.
- W2757655825 cites W2020055431 @default.
- W2757655825 cites W2020397881 @default.
- W2757655825 cites W2028185104 @default.
- W2757655825 cites W2030021883 @default.
- W2757655825 cites W2030193400 @default.
- W2757655825 cites W2031406182 @default.
- W2757655825 cites W2032415132 @default.
- W2757655825 cites W2046574179 @default.
- W2757655825 cites W2056116287 @default.
- W2757655825 cites W2060026300 @default.
- W2757655825 cites W2069241820 @default.
- W2757655825 cites W2072545281 @default.
- W2757655825 cites W2081292673 @default.
- W2757655825 cites W2083556180 @default.
- W2757655825 cites W2084021973 @default.
- W2757655825 cites W2088755036 @default.
- W2757655825 cites W2093940946 @default.
- W2757655825 cites W2094405531 @default.
- W2757655825 cites W2094595004 @default.
- W2757655825 cites W2097560724 @default.
- W2757655825 cites W2104934425 @default.
- W2757655825 cites W2106860332 @default.
- W2757655825 cites W2107531246 @default.
- W2757655825 cites W2108344673 @default.
- W2757655825 cites W2108569205 @default.
- W2757655825 cites W2111571980 @default.
- W2757655825 cites W2111903797 @default.
- W2757655825 cites W2114844963 @default.
- W2757655825 cites W2115299176 @default.
- W2757655825 cites W2115813995 @default.
- W2757655825 cites W2117846857 @default.
- W2757655825 cites W2119468962 @default.
- W2757655825 cites W2121644366 @default.
- W2757655825 cites W2125605543 @default.
- W2757655825 cites W2126924404 @default.
- W2757655825 cites W2127458887 @default.
- W2757655825 cites W2128350579 @default.
- W2757655825 cites W2128635872 @default.
- W2757655825 cites W2129629034 @default.
- W2757655825 cites W2130497216 @default.
- W2757655825 cites W2133425034 @default.
- W2757655825 cites W2137665452 @default.
- W2757655825 cites W2137959581 @default.
- W2757655825 cites W2140935709 @default.
- W2757655825 cites W2143498880 @default.
- W2757655825 cites W2144035662 @default.
- W2757655825 cites W2144236384 @default.
- W2757655825 cites W2144258074 @default.
- W2757655825 cites W2144956287 @default.
- W2757655825 cites W2146787746 @default.
- W2757655825 cites W2147614270 @default.
- W2757655825 cites W2149714948 @default.
- W2757655825 cites W2150097442 @default.
- W2757655825 cites W2151540993 @default.
- W2757655825 cites W2152348290 @default.
- W2757655825 cites W2154816058 @default.
- W2757655825 cites W2158351657 @default.
- W2757655825 cites W2161878252 @default.
- W2757655825 cites W2162295879 @default.
- W2757655825 cites W2162713037 @default.
- W2757655825 cites W2166830385 @default.