FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2758552779> ?p ?o ?g. }

• W2758552779 endingPage "11027" @default.
• W2758552779 startingPage "11018" @default.
• W2758552779 abstract "In this study, we examined the influence of bilayer thickness on the activity of the actinoporin toxins sticholysin I and II (StnI and StnII) at 25 °C. Bilayer thickness was varied using dimonounsaturated phosphatidylcholine (PC) analogues (with 14:1, 16:1, 18:1, 20:1, and 22:1 acyl chains). In addition, N-14:0-sphingomyelin (SM) was always included because StnI and StnII are SM specific. Cholesterol was also incorporated as indicated. In cholesterol-free large unilamellar vesicles (LUVs) the PC:SM molar ratio was 4:1, and when cholesterol was included, the complete molar ratio was 4:1:0.5 (PC:SM:cholesterol, respectively). Stn toxins promote bilayer leakage through pores formed by oligomerized toxin monomers. Initial calcein leakage was moderately dependent on bilayer PC acyl chain length (and thus bilayer thickness), with higher rates observed with di-16:1 and di-18:1 PC bilayers. In the presence of cholesterol, the maximum rates of calcein leakage were observed in di-14:1 and di-16:1 PC bilayers. Using isothermal titration calorimetry to study the Stn-LUV interaction, we observed that the bilayer affinity constant (Ka) peaked with LUVs containing di-18:1 PC, and was lower in shorter and longer PC acyl chain bilayers. The presence of cholesterol increased the binding affinity approximately 30-fold at the optimal bilayer thickness (di-18:1-PC). We conclude that bilayer thickness affects both functional and conformational aspects of Stn membrane binding and pore formation. Moreover, the length of the actinoporins' N-terminal α-helix, which penetrates the membrane to form a functional pore, appears to be optimal for the membrane thickness represented by di-18:1 PC." @default.
• W2758552779 created "2017-10-06" @default.
• W2758552779 creator A5039190451 @default.
• W2758552779 creator A5048160824 @default.
• W2758552779 creator A5070167104 @default.
• W2758552779 creator A5075323087 @default.
• W2758552779 creator A5075612265 @default.
• W2758552779 creator A5082492042 @default.
• W2758552779 date "2017-10-04" @default.
• W2758552779 modified "2023-10-03" @default.
• W2758552779 title "Differential Effect of Bilayer Thickness on Sticholysin Activity" @default.
• W2758552779 cites W1449218956 @default.
• W2758552779 cites W1486365785 @default.
• W2758552779 cites W1499593166 @default.
• W2758552779 cites W1540205595 @default.
• W2758552779 cites W1584730117 @default.
• W2758552779 cites W1670108590 @default.
• W2758552779 cites W1965844436 @default.
• W2758552779 cites W1967383045 @default.
• W2758552779 cites W1967514838 @default.
• W2758552779 cites W1969674471 @default.
• W2758552779 cites W1971048299 @default.
• W2758552779 cites W1979048239 @default.
• W2758552779 cites W1982975958 @default.
• W2758552779 cites W1986331992 @default.
• W2758552779 cites W1991146845 @default.
• W2758552779 cites W1999909402 @default.
• W2758552779 cites W2002459713 @default.
• W2758552779 cites W2004050313 @default.
• W2758552779 cites W2007876142 @default.
• W2758552779 cites W2008305695 @default.
• W2758552779 cites W2011877786 @default.
• W2758552779 cites W2016343967 @default.
• W2758552779 cites W2016528090 @default.
• W2758552779 cites W2017698288 @default.
• W2758552779 cites W2018532807 @default.
• W2758552779 cites W2020405150 @default.
• W2758552779 cites W2021551854 @default.
• W2758552779 cites W2023163561 @default.
• W2758552779 cites W2038497192 @default.
• W2758552779 cites W2045398409 @default.
• W2758552779 cites W2052069688 @default.
• W2758552779 cites W2054127621 @default.
• W2758552779 cites W2056235355 @default.
• W2758552779 cites W2059166957 @default.
• W2758552779 cites W2059571424 @default.
• W2758552779 cites W2063897278 @default.
• W2758552779 cites W2071948808 @default.
• W2758552779 cites W2074551088 @default.
• W2758552779 cites W2081846733 @default.
• W2758552779 cites W2090415455 @default.
• W2758552779 cites W2091615898 @default.
• W2758552779 cites W2102206503 @default.
• W2758552779 cites W2108269041 @default.
• W2758552779 cites W2115252051 @default.
• W2758552779 cites W2129388681 @default.
• W2758552779 cites W2129995310 @default.
• W2758552779 cites W2131284518 @default.
• W2758552779 cites W2132629607 @default.
• W2758552779 cites W2134068468 @default.
• W2758552779 cites W2138993728 @default.
• W2758552779 cites W2147869016 @default.
• W2758552779 cites W2152225923 @default.
• W2758552779 cites W2155921993 @default.
• W2758552779 cites W2183689972 @default.
• W2758552779 cites W2213825066 @default.
• W2758552779 cites W2293442890 @default.
• W2758552779 cites W2298209565 @default.
• W2758552779 cites W2306939891 @default.
• W2758552779 cites W2344824571 @default.
• W2758552779 cites W2511608695 @default.
• W2758552779 cites W2552941677 @default.
• W2758552779 cites W4210604196 @default.
• W2758552779 cites W4231342004 @default.
• W2758552779 doi "https://doi.org/10.1021/acs.langmuir.7b01765" @default.
• W2758552779 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28933861" @default.
• W2758552779 hasPublicationYear "2017" @default.
• W2758552779 type Work @default.
• W2758552779 sameAs 2758552779 @default.
• W2758552779 citedByCount "14" @default.
• W2758552779 countsByYear W27585527792019 @default.
• W2758552779 countsByYear W27585527792020 @default.
• W2758552779 countsByYear W27585527792021 @default.
• W2758552779 countsByYear W27585527792022 @default.
• W2758552779 countsByYear W27585527792023 @default.
• W2758552779 crossrefType "journal-article" @default.
• W2758552779 hasAuthorship W2758552779A5039190451 @default.
• W2758552779 hasAuthorship W2758552779A5048160824 @default.
• W2758552779 hasAuthorship W2758552779A5070167104 @default.
• W2758552779 hasAuthorship W2758552779A5075323087 @default.
• W2758552779 hasAuthorship W2758552779A5075612265 @default.
• W2758552779 hasAuthorship W2758552779A5082492042 @default.
• W2758552779 hasConcept C12554922 @default.
• W2758552779 hasConcept C130316041 @default.
• W2758552779 hasConcept C156911925 @default.
• W2758552779 hasConcept C185592680 @default.
• W2758552779 hasConcept C192157962 @default.
• W2758552779 hasConcept C2776330855 @default.

• next