FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2759675946> ?p ?o ?g. }

• W2759675946 endingPage "3343" @default.
• W2759675946 startingPage "3332" @default.
• W2759675946 abstract "Isothermal amplification techniques, which avoid thermal cycling, are often confounded by non-specific amplification arising from off-target hybrids, interactions between (hetero-dimer) or within (self-dimerization) primers and carryover contaminants. Here, we developed a new method, termed multiple cross displacement amplification Plus (MCDA Plus), which uses self-avoiding molecular recognition system (SAMRS) components and antarctic thermal sensitive uracil-DNA-glycosylase (AUDG) enzyme to improve the specificity of MCDA and prevent carryover contamination, respectively. In the presence of SAMRS components, the false positive results arising from off-target hybrids and undesired interactions between or within primers are completely eliminated, which increase the reproducibility of the assay. In the presence of AUDG, the false positive results generating from carryover contaminants are removed, thus the genuine positive results are obtained from the amplification of target sequences. Moreover, we also devised a new analysis strategy, which was intended for the detection of amplified products using lateral flow biosensor (LFB). The analysis system only required a single labeled primer, thus eliminated the false positive result yielding from hybridization (the labeled primer and probe, or between two labeled primers). Therefore, the SAMRS components, AUDG and LFB convert standard MCDA from an assay suited only for the research laboratory into one that has applicable value at points-of-care testing, in the field environments and in poor-resource settings. For demonstration purpose, Mycobacterium tuberculosis complex (MTC), which is the causes of human Tuberculosis (TB), are detected by the novel MCDA Plus technique to demonstrate the capability of target analysis. The performance of MCDA Plus assay in identifying MTC from pure culture and clinical samples is successfully evaluated. The proof-of-concept technique can be reconfigured to detect various nucleic acid sequences by redesigning the specific MCDA Plus primers." @default.
• W2759675946 created "2017-10-06" @default.
• W2759675946 creator A5004524880 @default.
• W2759675946 creator A5005283872 @default.
• W2759675946 creator A5009089176 @default.
• W2759675946 creator A5010057342 @default.
• W2759675946 creator A5040443849 @default.
• W2759675946 creator A5053164555 @default.
• W2759675946 date "2018-02-01" @default.
• W2759675946 modified "2023-10-16" @default.
• W2759675946 title "Nanoparticles-based lateral flow biosensor coupled with multiple cross displacement amplification Plus for simultaneous detection of nucleic acid and prevention of carryover contamination" @default.
• W2759675946 cites W1526111580 @default.
• W2759675946 cites W1984966664 @default.
• W2759675946 cites W1987176207 @default.
• W2759675946 cites W1995506594 @default.
• W2759675946 cites W1995711924 @default.
• W2759675946 cites W1997847658 @default.
• W2759675946 cites W1997947366 @default.
• W2759675946 cites W1997955480 @default.
• W2759675946 cites W2014748437 @default.
• W2759675946 cites W2039696994 @default.
• W2759675946 cites W2042044389 @default.
• W2759675946 cites W2058101641 @default.
• W2759675946 cites W2065098811 @default.
• W2759675946 cites W2069376630 @default.
• W2759675946 cites W2091848147 @default.
• W2759675946 cites W2094457052 @default.
• W2759675946 cites W2100538295 @default.
• W2759675946 cites W2104663976 @default.
• W2759675946 cites W2112607854 @default.
• W2759675946 cites W2142039600 @default.
• W2759675946 cites W2145303234 @default.
• W2759675946 cites W2150664840 @default.
• W2759675946 cites W2157503985 @default.
• W2759675946 cites W2169930144 @default.
• W2759675946 cites W2170849437 @default.
• W2759675946 cites W2330806411 @default.
• W2759675946 cites W2523407052 @default.
• W2759675946 cites W2527697921 @default.
• W2759675946 cites W2576294113 @default.
• W2759675946 cites W2614762071 @default.
• W2759675946 cites W2804026402 @default.
• W2759675946 cites W338663261 @default.
• W2759675946 doi "https://doi.org/10.1016/j.snb.2017.09.160" @default.
• W2759675946 hasPublicationYear "2018" @default.
• W2759675946 type Work @default.
• W2759675946 sameAs 2759675946 @default.
• W2759675946 citedByCount "8" @default.
• W2759675946 countsByYear W27596759462018 @default.
• W2759675946 countsByYear W27596759462019 @default.
• W2759675946 countsByYear W27596759462020 @default.
• W2759675946 countsByYear W27596759462021 @default.
• W2759675946 countsByYear W27596759462022 @default.
• W2759675946 crossrefType "journal-article" @default.
• W2759675946 hasAuthorship W2759675946A5004524880 @default.
• W2759675946 hasAuthorship W2759675946A5005283872 @default.
• W2759675946 hasAuthorship W2759675946A5009089176 @default.
• W2759675946 hasAuthorship W2759675946A5010057342 @default.
• W2759675946 hasAuthorship W2759675946A5040443849 @default.
• W2759675946 hasAuthorship W2759675946A5053164555 @default.
• W2759675946 hasConcept C104317684 @default.
• W2759675946 hasConcept C112570922 @default.
• W2759675946 hasConcept C156385826 @default.
• W2759675946 hasConcept C160756335 @default.
• W2759675946 hasConcept C178790620 @default.
• W2759675946 hasConcept C185592680 @default.
• W2759675946 hasConcept C18903297 @default.
• W2759675946 hasConcept C24107716 @default.
• W2759675946 hasConcept C2777563447 @default.
• W2759675946 hasConcept C43617362 @default.
• W2759675946 hasConcept C49105822 @default.
• W2759675946 hasConcept C552990157 @default.
• W2759675946 hasConcept C55493867 @default.
• W2759675946 hasConcept C60635243 @default.
• W2759675946 hasConcept C70721500 @default.
• W2759675946 hasConcept C78063203 @default.
• W2759675946 hasConcept C86803240 @default.
• W2759675946 hasConceptScore W2759675946C104317684 @default.
• W2759675946 hasConceptScore W2759675946C112570922 @default.
• W2759675946 hasConceptScore W2759675946C156385826 @default.
• W2759675946 hasConceptScore W2759675946C160756335 @default.
• W2759675946 hasConceptScore W2759675946C178790620 @default.
• W2759675946 hasConceptScore W2759675946C185592680 @default.
• W2759675946 hasConceptScore W2759675946C18903297 @default.
• W2759675946 hasConceptScore W2759675946C24107716 @default.
• W2759675946 hasConceptScore W2759675946C2777563447 @default.
• W2759675946 hasConceptScore W2759675946C43617362 @default.
• W2759675946 hasConceptScore W2759675946C49105822 @default.
• W2759675946 hasConceptScore W2759675946C552990157 @default.
• W2759675946 hasConceptScore W2759675946C55493867 @default.
• W2759675946 hasConceptScore W2759675946C60635243 @default.
• W2759675946 hasConceptScore W2759675946C70721500 @default.
• W2759675946 hasConceptScore W2759675946C78063203 @default.
• W2759675946 hasConceptScore W2759675946C86803240 @default.
• W2759675946 hasLocation W27596759461 @default.
• W2759675946 hasOpenAccess W2759675946 @default.
• W2759675946 hasPrimaryLocation W27596759461 @default.
• W2759675946 hasRelatedWork W2001725983 @default.

• next