FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2760024633> ?p ?o ?g. }

• W2760024633 endingPage "231" @default.
• W2760024633 startingPage "216" @default.
• W2760024633 abstract "Hyaluronic acid (HA) is a high-value biopolymer used in the biomedical, pharmaceutical, cosmetic, and food industries. Current methods of HA production, including extraction from animal sources and streptococcal cultivations, are associated with high costs and health risks. Accordingly, the development of bioprocesses for HA production centered on robust “Generally Recognized as Safe (GRAS)” organisms such as Bacillus subtilis is highly attractive. Here, we report the development of novel strains of B. subtilis in which the membrane cardiolipin (CL) content and distribution has been engineered to enhance the functional expression of heterologously expressed hyaluronan synthase (HAS) of Streptococcus equisimilis (SeHAS), in turn, improving the culture performance for HA production. Elevation of membrane CL levels via overexpressing components involved in the CL biosynthesis pathway, and redistribution of CL along the lateral membrane via repression of the cell division initiator protein FtsZ resulted in increases to the HA titer of up to 204% and peak molecular weight of up to 2.2 MDa. Moreover, removal of phosphatidylethanolamine and neutral glycolipids from the membrane of HA-producing B. subtilis via inactivation of pssA and ugtP, respectively, has suggested the lipid dependence for functional expression of SeHAS. Our study demonstrates successful application of membrane engineering strategies to develop an effective platform for biomanufacturing of HA with B. subtilis strains expressing Class I streptococcal HAS." @default.
• W2760024633 created "2017-10-06" @default.
• W2760024633 creator A5001705178 @default.
• W2760024633 creator A5055276776 @default.
• W2760024633 creator A5059914775 @default.
• W2760024633 creator A5081792027 @default.
• W2760024633 date "2017-10-23" @default.
• W2760024633 modified "2023-10-01" @default.
• W2760024633 title "Engineering of cell membrane to enhance heterologous production of hyaluronic acid in<i>Bacillus subtilis</i>" @default.
• W2760024633 cites W1499622424 @default.
• W2760024633 cites W1678318463 @default.
• W2760024633 cites W1963523663 @default.
• W2760024633 cites W1964995917 @default.
• W2760024633 cites W1966323379 @default.
• W2760024633 cites W1969817606 @default.
• W2760024633 cites W1973678713 @default.
• W2760024633 cites W1978310624 @default.
• W2760024633 cites W1978649824 @default.
• W2760024633 cites W1988726642 @default.
• W2760024633 cites W2001164692 @default.
• W2760024633 cites W2004369836 @default.
• W2760024633 cites W2005911673 @default.
• W2760024633 cites W2007224965 @default.
• W2760024633 cites W2008487520 @default.
• W2760024633 cites W2009570111 @default.
• W2760024633 cites W2013941038 @default.
• W2760024633 cites W2014357380 @default.
• W2760024633 cites W2016258598 @default.
• W2760024633 cites W2018639961 @default.
• W2760024633 cites W2022646939 @default.
• W2760024633 cites W2030159437 @default.
• W2760024633 cites W2034008313 @default.
• W2760024633 cites W2036938464 @default.
• W2760024633 cites W2044226086 @default.
• W2760024633 cites W2045973674 @default.
• W2760024633 cites W2046437093 @default.
• W2760024633 cites W2055592698 @default.
• W2760024633 cites W2061884945 @default.
• W2760024633 cites W2070528883 @default.
• W2760024633 cites W2070774028 @default.
• W2760024633 cites W2074875874 @default.
• W2760024633 cites W2075100243 @default.
• W2760024633 cites W2087704871 @default.
• W2760024633 cites W2090556419 @default.
• W2760024633 cites W2093763547 @default.
• W2760024633 cites W2099062342 @default.
• W2760024633 cites W2099540110 @default.
• W2760024633 cites W2100491597 @default.
• W2760024633 cites W2101040320 @default.
• W2760024633 cites W2102267945 @default.
• W2760024633 cites W2103686288 @default.
• W2760024633 cites W2104759129 @default.
• W2760024633 cites W2105719418 @default.
• W2760024633 cites W2109769274 @default.
• W2760024633 cites W2119044039 @default.
• W2760024633 cites W2121987040 @default.
• W2760024633 cites W2122238431 @default.
• W2760024633 cites W2126579809 @default.
• W2760024633 cites W2134370618 @default.
• W2760024633 cites W2136692871 @default.
• W2760024633 cites W2144544775 @default.
• W2760024633 cites W2149819424 @default.
• W2760024633 cites W2150804231 @default.
• W2760024633 cites W2155422793 @default.
• W2760024633 cites W2156783270 @default.
• W2760024633 cites W2160051211 @default.
• W2760024633 cites W2160235987 @default.
• W2760024633 cites W2163258998 @default.
• W2760024633 cites W2164997719 @default.
• W2760024633 cites W2169374580 @default.
• W2760024633 cites W2291204809 @default.
• W2760024633 cites W2416874763 @default.
• W2760024633 cites W2604172209 @default.
• W2760024633 cites W2612451420 @default.
• W2760024633 doi "https://doi.org/10.1002/bit.26459" @default.
• W2760024633 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28941282" @default.
• W2760024633 hasPublicationYear "2017" @default.
• W2760024633 type Work @default.
• W2760024633 sameAs 2760024633 @default.
• W2760024633 citedByCount "62" @default.
• W2760024633 countsByYear W27600246332018 @default.
• W2760024633 countsByYear W27600246332019 @default.
• W2760024633 countsByYear W27600246332020 @default.
• W2760024633 countsByYear W27600246332021 @default.
• W2760024633 countsByYear W27600246332022 @default.
• W2760024633 countsByYear W27600246332023 @default.
• W2760024633 crossrefType "journal-article" @default.
• W2760024633 hasAuthorship W2760024633A5001705178 @default.
• W2760024633 hasAuthorship W2760024633A5055276776 @default.
• W2760024633 hasAuthorship W2760024633A5059914775 @default.
• W2760024633 hasAuthorship W2760024633A5081792027 @default.
• W2760024633 hasBestOaLocation W27600246331 @default.
• W2760024633 hasConcept C181199279 @default.
• W2760024633 hasConcept C185592680 @default.
• W2760024633 hasConcept C2776330855 @default.
• W2760024633 hasConcept C2777272437 @default.
• W2760024633 hasConcept C2778918659 @default.
• W2760024633 hasConcept C2781170229 @default.

• next