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- W2760208233 abstract "Grapevine (Vitis vinifera L.) is one of the major crops in Turkey; production exceeded 3.5 million tons in 2015. In a vineyard survey conducted during June 2016, main and lateral vein clearing and yellowing as well as leaf deformations were observed on grapevines in the Adana province of Turkey. Total RNA was extracted from 45 grapevine leaf samples collected from 15 vineyards with ZR RNA MiniPrep™ (Zymo Research, USA), and cDNAs were synthesized using EasyScript Plus™ cDNA Synthesis kit (Abm, Canada) to test the samples for several grapevine RNA or DNA viruses. Reverse transcription (RT)-PCR assays were also conducted with the primer pairs BadnUp-6262 5’- GAA AGA CGA ACC CTT CAT CAT GAA G -3’ and BadnDo-6757 5’- CCC CAT CGA CAG CTC ACA AG -3’ which amplify a 495 bp region of Grapevine Roditis leaf discoloration-associated virus (GRLDaV) (Maliogka et al., 2015). Four plant samples (isolates B34, B36, B42, B50) collected from three different vineyards were found positive for GRLDaV. The PCR products were d..." @default.
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- W2760208233 date "2018-01-01" @default.
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- W2760208233 title "First Report of <i>Grapevine Roditis leaf discoloration-associated virus</i> Infecting Grapevine (<i>Vitis vinifera</i>) in Turkey" @default.
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- W2760208233 doi "https://doi.org/10.1094/pdis-06-17-0927-pdn" @default.
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