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• W2760520478 abstract "High energy collision processes for singly charged positive ions using an alkali metal target are confirmed, as a charge inversion mass spectrometry, to occur by electron transfers in successive collisions and the dissociation processes involve the formation of energy-selected neutral species from near-resonant neutralization with alkali metal targets. A doubly charged thermometer molecule was made to collide with alkali metal targets to give singly and doubly charged positive ions. The internal energy resulting from the electron transfer with the alkali metal target was very narrow and centered at a particular energy. This narrow internal energy distribution can be attributed to electron transfer by Landau-Zener potential crossing between the precursor ion and an alkali metal atom, and the coulombic repulsion between singly charged ions in the exit channel. A large cross section of more than 10-14 cm2 was estimated for high-energy electron transfer dissociation (HE-ETD). Doubly protonated phosphorylated peptides obtained by electrospray ionization were collided with Xe and Cs targets to give singly and doubly charged positive ions. Whereas doubly charged fragment ions resulting from CAD were dominant in the case of the Xe target, singly charged fragment ions resulting from ETD were dominant with the Cs target. HE-ETD using the Cs target provided all of the z-type ions by N-Cα bond cleavage without the loss of the phosphate groups. The results demonstrate that HE-ETD with an alkali metal target allowed the position of phosphorylation and the amino acid sequence of peptides with post translational modifications (PTM) to be determined." @default.
• W2760520478 created "2017-10-06" @default.
• W2760520478 creator A5073739569 @default.
• W2760520478 date "2017-01-01" @default.
• W2760520478 modified "2023-09-25" @default.
• W2760520478 title "Study of Ion Dynamics by Electron Transfer Dissociation: Alkali Metals as Targets" @default.
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• W2760520478 doi "https://doi.org/10.5702/massspectrometry.a0062" @default.
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