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- W2761035879 abstract "Evernia prunastri (L.) Ach, belonging to Parmeliaceae, is an important lichen species in Turkey. Previous studies was reported that E. prunastri have significant antioxidant, antimicrobial and anticancer compounds in its structure. Although antiproliferative effects of E. prunastri are determined in some types of cancer, there is little information about the pathway of this activity. Accordingly, we aimed to determine the association between anti-proliferative activities of E. prunastri extracts with the cellular apoptotic pathway. Determination of cell viability was assessed by MTT assay. The xCELLigence system was used to monitor cell proliferation. To reveal induction apoptosis, expression of apoptotic pathway proteins, F-actin staining, ANNEXIN V/propodium iodide (PI) staining was used. As a result, E. prunastri MeOH extracts were found to have anti-proliferative activities especially in high doses (p<0.05 for 1, 5 and 25 µg ml-1; p<0.01 for 125 µg ml-1). Also, the extracts were found to be not inducing apoptosis in flow cytometric measurements. In addition, there was no significant decrease or increase in protein expressions of apoptotic pathway proteins. Lastly, density of actin filaments was not affected. MeOH extract of E. prunastri was found to have anti-proliferative activities, yet these anti-proliferative activities were not related to cellular apoptotic pathways." @default.
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- W2761035879 date "2017-09-30" @default.
- W2761035879 modified "2023-10-16" @default.
- W2761035879 title "Association Between Anti-Proliferative Activity of Evernia Prunastri with the Cellular Apoptotic Pathway" @default.
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- W2761035879 doi "https://doi.org/10.17776/csj.340502" @default.
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