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- W2761457519 abstract "This study reports a novel bio-bar-code amplification competitive immunoassay for the detection of pesticide residue combined with a micro well plate. The sensing competitive immunoassay was made up of two types of particles: (1) the gold nanoparticles (AuNPs) with recognition elements (monoclonal antibody: McAb) and hundreds of thiolated single-strand oligonucleotide bar-codes; and (2) the magnetic microparticle (MMPs) with competition elements (hapten-OVA) for the target pesticide. Target pesticide and pesticide hapten competed with McAb labeled on the surface of AuNPs. The bar-code strands were released by dithiothreitol. Then a simple method based on micro well plate was used for the quantification of the bar-code strands. The bar-code strands with the half-complementary biotinylated DNA and half-complementary DNA-modified gold nanoparticles probe were immobilized onto the surface of a streptavidin-coated micro well plate, and the signal was amplified by silver enhancement. Triazophos (TAP), as a pesticide model, was detected by the proposed immunoassay. The linear range was from 2.5 × 10−2 to 40.0 ng mL−1, and LOD of the immunoassay was 1.96 × 10−2 ng mL−1. The correlation was further studied between this method and GC–MS analysis. Therefore, this method provided a promising approach for rapid detection and screening of pesticide residue." @default.
- W2761457519 created "2017-10-20" @default.
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- W2761457519 date "2018-03-01" @default.
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- W2761457519 title "Highly sensitive detection of triazophos pesticide using a novel bio-bar-code amplification competitive immunoassay in a micro well plate-based platform" @default.
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- W2761457519 doi "https://doi.org/10.1016/j.snb.2017.10.075" @default.
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