FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2761771736> ?p ?o ?g. }

• W2761771736 abstract "Tripterygium wilfordii produces not only ent-kaurene, which is an intermediate of gibberellin biosynthesis in flowering plants, but also 16α-hydroxy-ent-kaurane, whose physiological role has not been characterized. The two compounds are biosynthesized from the universal diterpenoid precursor (E,E,E)-geranylgeranyl diphosphate (GGPP) by diterpene synthases, which have been discovered and functionally characterized in T. wilfordii. Here, we described the functional characterization of four cytochrome P450 reductases (TwCPR) and one ent-kaurene oxidase (TwKO). Four TwCPRs were found to have relatively ubiquitous expression in T. wilfordii root, stem, leaf, and flower tissues. Co-expression of both a TwCPR and TwKO in yeast showed that TwCPR3 has a slightly better activity for providing the electrons required for these reactions, indicating that TwCPR3 is more suitable for use in the functional analysis of other cytochrome P450 monooxygenases. TwKO catalyzed the three-step oxidation of the C4α methyl of the tetracyclic diterpene intermediate ent-kaurene to form ent-kaurenoic acid as an early step in gibberellin (GA) biosynthesis. Notably, TwKO could also convert 16α-hydroxy-ent-kaurane to 16α-hydroxy-ent-kauranoic acid, indicating an important function of 16α-hydroxy-ent-kaurane in the anti-HIV principle tripterifordin biosynthetic pathway in planta. Homology modelling and molecular docking were used to investigate the unknown crucial active amino acid residue involved in the catalytic reaction of TwKO, and one key residue (Leu387) contributed to the formation of 16α-hydroxy-ent-kauranoic acid, most likely by forming hydrogen bonds with the hydroxyl group (-OH) of 16α-hydroxy-ent-kaurane, which laid the basis for further investigation of the multifunctional nature of KO catalysis. Also, our findings paved the way for the complete biosynthesis of the anti-HIV principle tripterifordin." @default.
• W2761771736 created "2017-10-20" @default.
• W2761771736 creator A5013678096 @default.
• W2761771736 creator A5017313263 @default.
• W2761771736 creator A5019190057 @default.
• W2761771736 creator A5038399698 @default.
• W2761771736 creator A5039811866 @default.
• W2761771736 creator A5048822772 @default.
• W2761771736 creator A5048974339 @default.
• W2761771736 creator A5072035000 @default.
• W2761771736 creator A5074678706 @default.
• W2761771736 creator A5078062377 @default.
• W2761771736 creator A5081788625 @default.
• W2761771736 creator A5082816482 @default.
• W2761771736 creator A5089822225 @default.
• W2761771736 date "2017-10-13" @default.
• W2761771736 modified "2023-10-15" @default.
• W2761771736 title "Probing the Single Key Amino Acid Responsible for the Novel Catalytic Function of ent-Kaurene Oxidase Supported by NADPH-Cytochrome P450 Reductases in Tripterygium wilfordii" @default.
• W2761771736 cites W1208816652 @default.
• W2761771736 cites W1559356085 @default.
• W2761771736 cites W1904809696 @default.
• W2761771736 cites W1916698766 @default.
• W2761771736 cites W1967509668 @default.
• W2761771736 cites W1978875442 @default.
• W2761771736 cites W1984356602 @default.
• W2761771736 cites W1985504443 @default.
• W2761771736 cites W1997820716 @default.
• W2761771736 cites W2002104577 @default.
• W2761771736 cites W2011162627 @default.
• W2761771736 cites W2012190292 @default.
• W2761771736 cites W2016649780 @default.
• W2761771736 cites W2018989832 @default.
• W2761771736 cites W2023279198 @default.
• W2761771736 cites W2035932588 @default.
• W2761771736 cites W2039305969 @default.
• W2761771736 cites W2042066399 @default.
• W2761771736 cites W2048970513 @default.
• W2761771736 cites W2064931754 @default.
• W2761771736 cites W2069123601 @default.
• W2761771736 cites W2080029338 @default.
• W2761771736 cites W2082973532 @default.
• W2761771736 cites W2085760714 @default.
• W2761771736 cites W2088096487 @default.
• W2761771736 cites W2092009497 @default.
• W2761771736 cites W2099752571 @default.
• W2761771736 cites W2101364071 @default.
• W2761771736 cites W2107277218 @default.
• W2761771736 cites W2108460212 @default.
• W2761771736 cites W2116365597 @default.
• W2761771736 cites W2117318229 @default.
• W2761771736 cites W2123912338 @default.
• W2761771736 cites W2132304174 @default.
• W2761771736 cites W2134525392 @default.
• W2761771736 cites W2145922781 @default.
• W2761771736 cites W2148185400 @default.
• W2761771736 cites W2152207030 @default.
• W2761771736 cites W2155671268 @default.
• W2761771736 cites W2156654220 @default.
• W2761771736 cites W2168770860 @default.
• W2761771736 cites W2192080449 @default.
• W2761771736 cites W2223798378 @default.
• W2761771736 cites W2273718285 @default.
• W2761771736 cites W2289724655 @default.
• W2761771736 cites W2299561689 @default.
• W2761771736 cites W2300069084 @default.
• W2761771736 cites W2405886223 @default.
• W2761771736 cites W2547285063 @default.
• W2761771736 cites W2551726910 @default.
• W2761771736 cites W2555422457 @default.
• W2761771736 cites W2580758420 @default.
• W2761771736 cites W2592897013 @default.
• W2761771736 cites W2605397067 @default.
• W2761771736 cites W2625442522 @default.
• W2761771736 cites W2739540666 @default.
• W2761771736 doi "https://doi.org/10.3389/fpls.2017.01756" @default.
• W2761771736 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5645531" @default.
• W2761771736 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29081786" @default.
• W2761771736 hasPublicationYear "2017" @default.
• W2761771736 type Work @default.
• W2761771736 sameAs 2761771736 @default.
• W2761771736 citedByCount "19" @default.
• W2761771736 countsByYear W27617717362018 @default.
• W2761771736 countsByYear W27617717362019 @default.
• W2761771736 countsByYear W27617717362020 @default.
• W2761771736 countsByYear W27617717362021 @default.
• W2761771736 countsByYear W27617717362022 @default.
• W2761771736 countsByYear W27617717362023 @default.
• W2761771736 crossrefType "journal-article" @default.
• W2761771736 hasAuthorship W2761771736A5013678096 @default.
• W2761771736 hasAuthorship W2761771736A5017313263 @default.
• W2761771736 hasAuthorship W2761771736A5019190057 @default.
• W2761771736 hasAuthorship W2761771736A5038399698 @default.
• W2761771736 hasAuthorship W2761771736A5039811866 @default.
• W2761771736 hasAuthorship W2761771736A5048822772 @default.
• W2761771736 hasAuthorship W2761771736A5048974339 @default.
• W2761771736 hasAuthorship W2761771736A5072035000 @default.
• W2761771736 hasAuthorship W2761771736A5074678706 @default.
• W2761771736 hasAuthorship W2761771736A5078062377 @default.
• W2761771736 hasAuthorship W2761771736A5081788625 @default.
• W2761771736 hasAuthorship W2761771736A5082816482 @default.

• next