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- W2761852003 abstract "Polysaccharide is one of the important active ingredients of Cantharellus cibarius . The aims of this work were to analyze preliminary characterization and to investigate immunostimulating activity of a novel water-soluble neutral polysaccharide named JP1, which was purified from the fruiting body of Cantharellus cibarius using DEAE-FF chromatography and Sephadex G-100 chromatography. The characteristics of JP1 were determined by HPGPC, FT-IR spectra, gas chromatography, and Congo Red Method. Immunostimulating activity of JP1 was investigated in RAW264.7 cells. Results indicated that JP1 consisted of L-Arabinose, D-Mannose, D-Glucose, and D-Galactose in a molar ratio of 1 : 1.06 : 1.95 : 1.17 with a molecular weight of 336 kDa. JP1 is nontoxic to RAW264.7 cells at this concentration range (62.5–1000 μ g/mL). Furthermore, JP1 can promote mouse peritoneal macrophages to secrete NO and enhance the secretion of macrophages’ cytokines IL-6 in RAW264.7 cells. These results suggested that JP1 could have potential immunostimulating activity applications as medicine or functional food." @default.
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- W2761852003 date "2017-01-01" @default.
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- W2761852003 title "Purification and Structural Characterization of a Novel Water-Soluble Neutral Polysaccharide from<i> Cantharellus cibarius</i> and Its Immunostimulating Activity in RAW264.7 Cells" @default.
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- W2761852003 doi "https://doi.org/10.1155/2017/3074915" @default.
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