FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2761900535> ?p ?o ?g. }

• W2761900535 abstract "The molecular mechanisms underlying the biodegradation of N-methylpyrrolidone (NMP), a widely used industrial solvent that produces skin irritation in humans and is teratogenic in rats, are unknown. Alicycliphilus sp. strain BQ1 degrades NMP. By studying a transposon-tagged mutant unable to degrade NMP, we identified a six-gene cluster (nmpABCDEF) that is transcribed as a polycistronic mRNA and encodes enzymes involved in NMP biodegradation. nmpA and the transposon-affected gene nmpB encode an N-methylhydantoin amidohydrolase that transforms NMP to γ-N-methylaminobutyric acid; this is metabolized by an amino acid oxidase (NMPC), either by demethylation to produce γ-aminobutyric acid (GABA) or by deamination to produce succinate semialdehyde (SSA). If GABA is produced, the activity of a GABA aminotransferase (GABA-AT), not encoded in the nmp gene cluster, is needed to generate SSA. SSA is transformed by a succinate semialdehyde dehydrogenase (SSDH) (NMPF) to succinate, which enters the Krebs cycle. The abilities to consume NMP and to utilize it for growth were complemented in the transposon-tagged mutant by use of the nmpABCD genes. Similarly, Escherichia coli MG1655, which has two SSDHs but is unable to grow in NMP, acquired these abilities after functional complementation with these genes. In wild-type (wt) BQ1 cells growing in NMP, GABA was not detected, but SSA was present at double the amount found in cells growing in Luria-Bertani medium (LB), suggesting that GABA is not an intermediate in this pathway. Moreover, E. coli GABA-AT deletion mutants complemented with nmpABCD genes retained the ability to grow in NMP, supporting the possibility that γ-N-methylaminobutyric acid is deaminated to SSA instead of being demethylated to GABA.IMPORTANCEN-Methylpyrrolidone is a cyclic amide reported to be biodegradable. However, the metabolic pathway and enzymatic activities for degrading NMP are unknown. By developing molecular biology techniques for Alicycliphilus sp. strain BQ1, an environmental bacterium able to grow in NMP, we identified a six-gene cluster encoding enzymatic activities involved in NMP degradation. These findings set the basis for the study of new enzymatic activities and for the development of biotechnological processes with potential applications in bioremediation." @default.
• W2761900535 created "2017-10-20" @default.
• W2761900535 creator A5021424510 @default.
• W2761900535 creator A5023641285 @default.
• W2761900535 creator A5045270538 @default.
• W2761900535 creator A5045350665 @default.
• W2761900535 creator A5051269129 @default.
• W2761900535 creator A5058561378 @default.
• W2761900535 creator A5085241616 @default.
• W2761900535 creator A5090405636 @default.
• W2761900535 date "2018-01-01" @default.
• W2761900535 modified "2023-10-17" @default.
• W2761900535 title "Novel Metabolic Pathway for <i>N</i> -Methylpyrrolidone Degradation in Alicycliphilus sp. Strain BQ1" @default.
• W2761900535 cites W1556605011 @default.
• W2761900535 cites W1636002209 @default.
• W2761900535 cites W1796926218 @default.
• W2761900535 cites W1824614244 @default.
• W2761900535 cites W1943646322 @default.
• W2761900535 cites W1968183452 @default.
• W2761900535 cites W1969869085 @default.
• W2761900535 cites W1971167763 @default.
• W2761900535 cites W1980390304 @default.
• W2761900535 cites W2000268335 @default.
• W2761900535 cites W2001728937 @default.
• W2761900535 cites W2002186270 @default.
• W2761900535 cites W2002463127 @default.
• W2761900535 cites W2014361215 @default.
• W2761900535 cites W2015498234 @default.
• W2761900535 cites W2022966294 @default.
• W2761900535 cites W2027229485 @default.
• W2761900535 cites W2029314744 @default.
• W2761900535 cites W2029352805 @default.
• W2761900535 cites W2036917159 @default.
• W2761900535 cites W2042913108 @default.
• W2761900535 cites W2049365572 @default.
• W2761900535 cites W2049417568 @default.
• W2761900535 cites W2072585559 @default.
• W2761900535 cites W2083856798 @default.
• W2761900535 cites W2088345538 @default.
• W2761900535 cites W2088438277 @default.
• W2761900535 cites W2093660878 @default.
• W2761900535 cites W2095873487 @default.
• W2761900535 cites W2098822140 @default.
• W2761900535 cites W2099408211 @default.
• W2761900535 cites W2099652776 @default.
• W2761900535 cites W2101115187 @default.
• W2761900535 cites W2110597772 @default.
• W2761900535 cites W2111967267 @default.
• W2761900535 cites W2116137883 @default.
• W2761900535 cites W2117609742 @default.
• W2761900535 cites W2122464816 @default.
• W2761900535 cites W2128436849 @default.
• W2761900535 cites W2132417094 @default.
• W2761900535 cites W2132666490 @default.
• W2761900535 cites W2136274981 @default.
• W2761900535 cites W2136670217 @default.
• W2761900535 cites W2141301529 @default.
• W2761900535 cites W2149341297 @default.
• W2761900535 cites W2153503474 @default.
• W2761900535 cites W2155690692 @default.
• W2761900535 cites W2156504093 @default.
• W2761900535 cites W2158488384 @default.
• W2761900535 cites W2158937196 @default.
• W2761900535 cites W2160191056 @default.
• W2761900535 cites W2163438467 @default.
• W2761900535 cites W2164080257 @default.
• W2761900535 cites W2171604982 @default.
• W2761900535 cites W2322957911 @default.
• W2761900535 cites W2324173565 @default.
• W2761900535 doi "https://doi.org/10.1128/aem.02136-17" @default.
• W2761900535 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5734037" @default.
• W2761900535 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29030443" @default.
• W2761900535 hasPublicationYear "2018" @default.
• W2761900535 type Work @default.
• W2761900535 sameAs 2761900535 @default.
• W2761900535 citedByCount "8" @default.
• W2761900535 countsByYear W27619005352019 @default.
• W2761900535 countsByYear W27619005352020 @default.
• W2761900535 countsByYear W27619005352022 @default.
• W2761900535 countsByYear W27619005352023 @default.
• W2761900535 crossrefType "journal-article" @default.
• W2761900535 hasAuthorship W2761900535A5021424510 @default.
• W2761900535 hasAuthorship W2761900535A5023641285 @default.
• W2761900535 hasAuthorship W2761900535A5045270538 @default.
• W2761900535 hasAuthorship W2761900535A5045350665 @default.
• W2761900535 hasAuthorship W2761900535A5051269129 @default.
• W2761900535 hasAuthorship W2761900535A5058561378 @default.
• W2761900535 hasAuthorship W2761900535A5085241616 @default.
• W2761900535 hasAuthorship W2761900535A5090405636 @default.
• W2761900535 hasBestOaLocation W27619005351 @default.
• W2761900535 hasConcept C104317684 @default.
• W2761900535 hasConcept C143065580 @default.
• W2761900535 hasConcept C153911025 @default.
• W2761900535 hasConcept C181199279 @default.
• W2761900535 hasConcept C188082640 @default.
• W2761900535 hasConcept C2779485144 @default.
• W2761900535 hasConcept C2781330172 @default.
• W2761900535 hasConcept C28693550 @default.
• W2761900535 hasConcept C36857842 @default.
• W2761900535 hasConcept C4918238 @default.

• next