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• W2762506088 abstract "Extracellular matrix (ECM) microenvironment is critical for the viability, stemness, and differentiation of stem cells. In this study, we developed hybrid-spheroids of human turbinate mesenchymal stem cells (hTMSCs) by using extracellular matrix (ECM) mimicking fragmented fibers (FFs) for improvement of the viability and functions of hTMSCs. We prepared FFs with average size of 68.26 µm by partial aminolysis of poly L-lactide (PLLA) fibrous sheet (FS), which was coated with polydopamine for improved cell adhesion. The proliferation of hTMSCs within the hybrid-spheroids mixed with fragmented fibers was significantly increased as compared to that from the cell-only group. Cells and fragmented fibers were homogenously distributed with the presence of pore like empty spaces in the structure. LOX-1 staining revealed that the hybrid-spheroids improved the cell viability, which was potentially due to enhanced transport of oxygen through void space generated by engineered ECM. Transmission electron microscopy (TEM) analysis confirmed that cells within the hybrid-spheroid formed strong cell junctions and contacts with fragmented fibers. The expression of cell junction proteins including connexin 43 and E-cadherin was significantly upregulated in hybrid-spheroids by 16.53 ± 0.04 and 28.26 ± 0.11-fold greater than that from cell-only group. Similarly, expression of integrin α2, α5, and β1 was significantly enhanced at the same group by 25.72 ± 0.13, 27.48 ± 0.49, and 592.78 ± 0.06-fold, respectively. In addition, stemness markers including Oct-4, Nanog, and Sox2 were significantly upregulated in hybrid-spheroids by 96.56 ± 0.06, 158.95 ± 0.06, and 115.46 ± 0.47-fold, respectively, relative to the cell-only group. Additionally, hTMSCs within the hybrid-spheroids showed significantly greater osteogenic differentiation under osteogenic media conditions. Taken together, our hybrid-spheroids can be an ideal approach for stem cell expansion and serve as a potential carrier for bone regeneration.Cells are spatially arranged within extracellular matrix (ECM) and cell/ECM interactions are crucial for cellular functions. Here, we developed a hybrid-spheroid system incorporating engineered ECM prepared from fragmented electrospun fibers to tune stem cell functions. Conventionally prepared cell spheroids with large diameters (>200 µm) is often prone to hypoxia. In contrast, the hybrid-spheroids significantly enhanced viability and proliferation of human turbinate mesenchymal stem cells (hTMSCs) as compared to spheroid prepared from cell only. Under these conditions, the presence of fragmented fibers also improved maintenance of stemness of hTMSCs for longer time cultured in growth media and demonstrated significantly greater osteogenic differentiation under osteogenic media conditions. Thus, the hybrid-spheroids can be used as a delivery carrier for stem cell based therapy or a 3D culture model for in vitro assay." @default.
• W2762506088 created "2017-10-20" @default.
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• W2762506088 date "2017-12-01" @default.
• W2762506088 modified "2023-10-02" @default.
• W2762506088 title "Hybrid-spheroids incorporating ECM like engineered fragmented fibers potentiate stem cell function by improved cell/cell and cell/ECM interactions" @default.
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• W2762506088 cites W1884433770 @default.
• W2762506088 cites W1965440553 @default.
• W2762506088 cites W1965975485 @default.
• W2762506088 cites W1974282501 @default.
• W2762506088 cites W1975401165 @default.
• W2762506088 cites W1983197681 @default.
• W2762506088 cites W1985658830 @default.
• W2762506088 cites W1990677265 @default.
• W2762506088 cites W1993982785 @default.
• W2762506088 cites W1994423139 @default.
• W2762506088 cites W1994966688 @default.
• W2762506088 cites W1995526109 @default.
• W2762506088 cites W2000898725 @default.
• W2762506088 cites W2001582495 @default.
• W2762506088 cites W2002145511 @default.
• W2762506088 cites W2007594132 @default.
• W2762506088 cites W2009035128 @default.
• W2762506088 cites W2010199370 @default.
• W2762506088 cites W2017145276 @default.
• W2762506088 cites W2017976179 @default.
• W2762506088 cites W2021011246 @default.
• W2762506088 cites W2022345246 @default.
• W2762506088 cites W2027384096 @default.
• W2762506088 cites W2029675195 @default.
• W2762506088 cites W2030178482 @default.
• W2762506088 cites W2030305442 @default.
• W2762506088 cites W2040810212 @default.
• W2762506088 cites W2053442544 @default.
• W2762506088 cites W2054409347 @default.
• W2762506088 cites W2056104115 @default.
• W2762506088 cites W2064404217 @default.
• W2762506088 cites W2070306500 @default.
• W2762506088 cites W2074526547 @default.
• W2762506088 cites W2074661784 @default.
• W2762506088 cites W2081581508 @default.
• W2762506088 cites W2085115041 @default.
• W2762506088 cites W2108038175 @default.
• W2762506088 cites W2108578973 @default.
• W2762506088 cites W2111648299 @default.
• W2762506088 cites W2112980849 @default.
• W2762506088 cites W2121210310 @default.
• W2762506088 cites W2124948262 @default.
• W2762506088 cites W2126737427 @default.
• W2762506088 cites W2131631451 @default.
• W2762506088 cites W2132559891 @default.
• W2762506088 cites W2135325454 @default.
• W2762506088 cites W2137050329 @default.
• W2762506088 cites W2138030809 @default.
• W2762506088 cites W2152459707 @default.
• W2762506088 cites W2191932260 @default.
• W2762506088 cites W2207416616 @default.
• W2762506088 cites W2228561711 @default.
• W2762506088 cites W2238333901 @default.
• W2762506088 cites W2331628193 @default.
• W2762506088 cites W2520900466 @default.
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• W2762506088 cites W2589861178 @default.
• W2762506088 cites W2602933976 @default.
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• W2762506088 doi "https://doi.org/10.1016/j.actbio.2017.10.022" @default.
• W2762506088 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29037892" @default.
• W2762506088 hasPublicationYear "2017" @default.
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