FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2762536872> ?p ?o ?g. }

• W2762536872 abstract "Abstract Chromosome engineering techniques including gene insertion, telomere-associated truncation and microcell-mediated chromosome transfer (MMCT) are powerful tools for generation of humanised model animal, containing megabase-sized genomic fragments. However, these techniques require two cell lines: homologous recombination (HR)-proficient DT40 cells for chromosome modification, and CHO cells for transfer to recipient cells. Here we show an improved technique using a combination of CRISPR/Cas9-induced HR in CHO and mouse A9 cells without DT40 cells following MMCT to recipient cells. Transgene insertion was performed in CHO cells with the insertion of enhanced green fluorescence protein (EGFP) using CRISPR/Cas9 and a circular targeting vector containing two 3 kb HR arms. Telomere-associated truncation was performed in CHO cells using CRISPR/Cas9 and a linearised truncation vector containing a single 7 kb HR arm at the 5′ end, a 1 kb artificial telomere at the 3′ end. At least 11% and 6% of the targeting efficiency were achieved for transgene insertion and telomere-associated truncation, respectively. The transgene insertion was also confirmed in A9 cells (29%). The modified chromosomes were transferrable to other cells. Thus, this CHO and A9 cell-mediated chromosome engineering using the CRISPR/Cas9 for direct transfer of the modified chromosome is a rapid technique that will facilitate chromosome manipulation." @default.
• W2762536872 created "2017-10-20" @default.
• W2762536872 creator A5004986891 @default.
• W2762536872 creator A5037810503 @default.
• W2762536872 creator A5049283637 @default.
• W2762536872 creator A5063443220 @default.
• W2762536872 creator A5081750464 @default.
• W2762536872 creator A5089695524 @default.
• W2762536872 date "2017-10-06" @default.
• W2762536872 modified "2023-10-06" @default.
• W2762536872 title "CRISPR/Cas9-induced transgene insertion and telomere-associated truncation of a single human chromosome for chromosome engineering in CHO and A9 cells" @default.
• W2762536872 cites W1519336129 @default.
• W2762536872 cites W1541417296 @default.
• W2762536872 cites W1965618950 @default.
• W2762536872 cites W1980449150 @default.
• W2762536872 cites W1981540403 @default.
• W2762536872 cites W1990029503 @default.
• W2762536872 cites W2007786482 @default.
• W2762536872 cites W2008147758 @default.
• W2762536872 cites W2016637438 @default.
• W2762536872 cites W2020321482 @default.
• W2762536872 cites W2034419972 @default.
• W2762536872 cites W2036294121 @default.
• W2762536872 cites W2037870786 @default.
• W2762536872 cites W2049974202 @default.
• W2762536872 cites W2050480410 @default.
• W2762536872 cites W2061184966 @default.
• W2762536872 cites W2064277868 @default.
• W2762536872 cites W2082745201 @default.
• W2762536872 cites W2117763379 @default.
• W2762536872 cites W2119283422 @default.
• W2762536872 cites W2124132056 @default.
• W2762536872 cites W2130005269 @default.
• W2762536872 cites W2133211693 @default.
• W2762536872 cites W2135725199 @default.
• W2762536872 cites W2145034938 @default.
• W2762536872 cites W2154780255 @default.
• W2762536872 cites W2163938277 @default.
• W2762536872 cites W2165998489 @default.
• W2762536872 cites W2167380395 @default.
• W2762536872 cites W2170039745 @default.
• W2762536872 cites W2208298285 @default.
• W2762536872 cites W2316431756 @default.
• W2762536872 cites W2466365188 @default.
• W2762536872 cites W2467980536 @default.
• W2762536872 doi "https://doi.org/10.1038/s41598-017-10418-7" @default.
• W2762536872 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5630592" @default.
• W2762536872 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28986519" @default.
• W2762536872 hasPublicationYear "2017" @default.
• W2762536872 type Work @default.
• W2762536872 sameAs 2762536872 @default.
• W2762536872 citedByCount "16" @default.
• W2762536872 countsByYear W27625368722017 @default.
• W2762536872 countsByYear W27625368722018 @default.
• W2762536872 countsByYear W27625368722019 @default.
• W2762536872 countsByYear W27625368722020 @default.
• W2762536872 countsByYear W27625368722021 @default.
• W2762536872 countsByYear W27625368722022 @default.
• W2762536872 countsByYear W27625368722023 @default.
• W2762536872 crossrefType "journal-article" @default.
• W2762536872 hasAuthorship W2762536872A5004986891 @default.
• W2762536872 hasAuthorship W2762536872A5037810503 @default.
• W2762536872 hasAuthorship W2762536872A5049283637 @default.
• W2762536872 hasAuthorship W2762536872A5063443220 @default.
• W2762536872 hasAuthorship W2762536872A5081750464 @default.
• W2762536872 hasAuthorship W2762536872A5089695524 @default.
• W2762536872 hasBestOaLocation W27625368721 @default.
• W2762536872 hasConcept C102230213 @default.
• W2762536872 hasConcept C102744134 @default.
• W2762536872 hasConcept C104317684 @default.
• W2762536872 hasConcept C132455925 @default.
• W2762536872 hasConcept C145290725 @default.
• W2762536872 hasConcept C153911025 @default.
• W2762536872 hasConcept C175656101 @default.
• W2762536872 hasConcept C177336024 @default.
• W2762536872 hasConcept C30481170 @default.
• W2762536872 hasConcept C54355233 @default.
• W2762536872 hasConcept C81885089 @default.
• W2762536872 hasConcept C86803240 @default.
• W2762536872 hasConcept C95444343 @default.
• W2762536872 hasConcept C98108389 @default.
• W2762536872 hasConceptScore W2762536872C102230213 @default.
• W2762536872 hasConceptScore W2762536872C102744134 @default.
• W2762536872 hasConceptScore W2762536872C104317684 @default.
• W2762536872 hasConceptScore W2762536872C132455925 @default.
• W2762536872 hasConceptScore W2762536872C145290725 @default.
• W2762536872 hasConceptScore W2762536872C153911025 @default.
• W2762536872 hasConceptScore W2762536872C175656101 @default.
• W2762536872 hasConceptScore W2762536872C177336024 @default.
• W2762536872 hasConceptScore W2762536872C30481170 @default.
• W2762536872 hasConceptScore W2762536872C54355233 @default.
• W2762536872 hasConceptScore W2762536872C81885089 @default.
• W2762536872 hasConceptScore W2762536872C86803240 @default.
• W2762536872 hasConceptScore W2762536872C95444343 @default.
• W2762536872 hasConceptScore W2762536872C98108389 @default.
• W2762536872 hasIssue "1" @default.
• W2762536872 hasLocation W27625368721 @default.
• W2762536872 hasLocation W27625368722 @default.
• W2762536872 hasLocation W27625368723 @default.
• W2762536872 hasLocation W27625368724 @default.

• next