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- W2762959736 abstract "Abstract Water-window x-ray microscopy allows two- and three-dimensional (2D and 3D) imaging of intact unstained cells in their cryofixed near-native state with unique contrast and high resolution. Present operational biological water-window microscopes are based at synchrotron facilities, which limits their accessibility and integration with complementary methods. Laboratory-source microscopes have had difficulty addressing relevant biological tasks with proper resolution and contrast due to long exposure times and limited up-time. Here we report on laboratory cryo x-ray microscopy with the exposure time, contrast, and reliability to allow for routine high-spatial resolution 3D imaging of intact cells and cell-cell interactions. Stabilization of the laser-plasma source combined with new optics and sample preparation provide high-resolution cell imaging, both in 2D with ten-second exposures and in 3D with twenty-minute tomography. Examples include monitoring of the distribution of carbon-dense vesicles in starving HEK293T cells and imaging the interaction between natural killer cells and target cells." @default.
- W2762959736 created "2017-10-20" @default.
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- W2762959736 date "2017-10-18" @default.
- W2762959736 modified "2023-10-18" @default.
- W2762959736 title "Laboratory cryo x-ray microscopy for 3D cell imaging" @default.
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- W2762959736 doi "https://doi.org/10.1038/s41598-017-13538-2" @default.
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