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- W2763119360 abstract "Water temperature is an essential environmental factor in aquaculture that affects many aspects of organism, and a rise in water temperature stresses aquatic animals. HSP60 is a major component of the chaperone system and plays an important role in stress response. In this study, the full-length cDNA sequence of HSP60 from Marsupenaeus japonicus was cloned for the first time, and the tissue distributions and expression profiles of MjHSP60 at the early developmental stages of M. japonicus and under heat stress were verified by qRT-PCR. The full-length cDNA sequence of MjHSP60 was 2,303 bp with the deduced amino acid (AA) sequence of 579 AA. MjHSP60 was expressed in all eight tested tissues of M. japonicus and was mainly expressed in the hepatopancreas under normal condition. MjHSP60 was expressed in all of the early developmental stages examined from the fertilized egg to the post-larval stage, and the expression level of MjHSP60 peaked at zoea II and reached a trough at the transition periods between each two stages (N and M1) and showed a relatively stable expression level at the post-larval stage. The expression level of MjHSP60 was significantly up-regulated in the gills, muscle, heart, hepatopancreas and stomach at 3 hr post-heat-stress and showed varied sensitivity to heat stress. The expression profile of MjHSP60 in the hepatopancreas and gill showed a time-dependent manner post-heat-stress and peaked at 3 hr post-heat-stress. The present study provided a basis for further studies for elucidating the function of MjHSP60 in the heat stress response and the early developmental stages of M. japonicus." @default.
- W2763119360 created "2017-10-20" @default.
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- W2763119360 date "2017-10-10" @default.
- W2763119360 modified "2023-09-24" @default.
- W2763119360 title "Molecular cloning of heat shock protein 60 from <i>Marsupenaeus japonicus</i> and its expression profiles at early developmental stages and response to heat stress" @default.
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- W2763119360 doi "https://doi.org/10.1111/are.13461" @default.
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