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- W2763673181 abstract "This chapter introduces the quantitative PCR (qPCR) array, explores the advantages and disadvantages of the method, and reviews the literature on the research on wastewater treatment plants (WWTPs) and related environments utilizing qPCR arrays. Urban WWTPs have been considered hot spots for horizontal gene transfer (HGT) and they are recognized as key environments where antibiotic resistance evolves. WWTPs are one of the main point sources of antibiotic resistance to the environment, and the resistance has been found to be enriched by the treatment processes. The large and growing number of antibiotic resistance genes calls for high-throughput methods for surveillance of antibiotic resistance genes (ARGs) in anthropogenic environments.Quantitative PCR (qPCR) is a suitable method for quantifying the copy numbers or analyzing the abundances of ARGs or other genes of interest. The qPCR array chips or plates can hold numerous qPCR assays targeting up to hundreds of different genes, which are analyzed in a single run." @default.
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- W2763673181 date "2017-09-29" @default.
- W2763673181 modified "2023-10-17" @default.
- W2763673181 title "High Throughput Method for Analyzing Antibiotic Resistance Genes in Wastewater Treatment Plants" @default.
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- W2763673181 doi "https://doi.org/10.1002/9781119192428.ch14" @default.
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