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• W2763881719 abstract "The application of PAT for in-line monitoring of biopharmaceutical manufacturing operations has a central role in developing more robust and consistent processes. Various spectroscopic techniques have been applied for collecting real-time data from cell culture processes. Among these, Raman spectroscopy has been shown to have advantages over other spectroscopic techniques, especially in aqueous culture solutions. Measurements of several process parameters such as glucose, lactate, glutamine, glutamate, ammonium, osmolality and VCD using Raman-based chemometrics models have been reported in literature. The application of Raman spectroscopy, coupled with calibration models for amino acid measurement in cell cultures, has been assessed. The developed models cover four amino acids important for cell growth and production: tyrosine, tryptophan, phenylalanine and methionine. The chemometrics models based on Raman spectroscopy data demonstrate the significant potential for the quantification of tyrosine, tryptophan and phenylalanine. The model for methionine would have to be further refined to improve quantification." @default.
• W2763881719 created "2017-10-20" @default.
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• W2763881719 date "2017-10-26" @default.
• W2763881719 modified "2023-10-16" @default.
• W2763881719 title "In-line monitoring of amino acids in mammalian cell cultures using raman spectroscopy and multivariate chemometrics models" @default.
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• W2763881719 doi "https://doi.org/10.1002/elsc.201700084" @default.
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