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• W2764061522 abstract "Stasis zone is the surrounding area of the coagulation zone which is an important part determining the extent of the necrosis in burn patients. In our study we aim to salvage the stasis zone by injecting adipose derived stromal vascular fraction (ADSVF). Thermal injury was applied on dorsum of Sprague–Dawley rats (n = 20) by the “comb burn” model as described previously. When the burn injury was established on Sprague–Dawley rats (30 min); rat dorsum was separated into 2 equal parts consisting of 4 burn zones (3 stasis zone) on each pair. ADSVF cells harvested from inguinal fat pads of Sprague–Dawley rats (n = 5) were injected on the right side while same amount of phosphate buffered saline (PBS) injected on the left side of the same animal. One week later, average vital tissue on the statis zone was determined by macroscopy, angiography and microscopy. Vascular density, inflammatory cell density, gradient of fibrosis and epithelial thickness were determined via immunohistochemical assay. Macroscopic stasis zone tissue viability (32 ± 3.28%, 57 ± 4.28%) (p < 0.01), average number of vessels (10.28 ± 1.28, 19.43 ± 1.72) (p < 0.01), capillary count (15.67 ± 1.97, 25.35 ± 2.15) (p < 0.01) vascular density (1.55 ± 0.38, 2.14 ± 0.45) (p < 0.01) epithelial thickness (0.014 ± 0.009 mm, 0.024 ± 0.0011 mm) were higher on ADSVF side. Fibrosis gradient (1.87 ± 0.51, 1.50 ± 0.43) (p < 0.01) and inflammatory cell density (1.33 ± 0.40, 1.20 ± 0.32) (p < 0.01) were higher on the PBS side. Macroscopic and microscopic findings determined that ADSVF has a statistically significant benefit for salvaging stasis zone on acute burn injuries." @default.
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• W2764061522 date "2018-03-01" @default.
• W2764061522 modified "2023-09-24" @default.
• W2764061522 title "The effect of adipose derived stromal vascular fraction on stasis zone in an experimental burn model" @default.
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• W2764061522 doi "https://doi.org/10.1016/j.burns.2017.08.016" @default.
• W2764061522 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29032969" @default.
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