Matches in SemOpenAlex for { <https://semopenalex.org/work/W2764302512> ?p ?o ?g. }
- W2764302512 abstract "The ESCRT machinery mediates reverse membrane scission. By quantitative fluorescence lattice light-sheet microscopy, we have shown that ESCRT-III subunits polymerize rapidly on yeast endosomes, together with the recruitment of at least two Vps4 hexamers. During their 3–45 s lifetimes, the ESCRT-III assemblies accumulated 75–200 Snf7 and 15–50 Vps24 molecules. Productive budding events required at least two additional Vps4 hexamers. Membrane budding was associated with continuous, stochastic exchange of Vps4 and ESCRT-III components, rather than steady growth of fixed assemblies, and depended on Vps4 ATPase activity. An all-or-none step led to final release of ESCRT-III and Vps4. Tomographic electron microscopy demonstrated that acute disruption of Vps4 recruitment stalled membrane budding. We propose a model in which multiple Vps4 hexamers (four or more) draw together several ESCRT-III filaments. This process induces cargo crowding and inward membrane buckling, followed by constriction of the nascent bud neck and ultimately ILV generation by vesicle fission." @default.
- W2764302512 created "2017-10-20" @default.
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- W2764302512 date "2017-10-11" @default.
- W2764302512 modified "2023-10-16" @default.
- W2764302512 title "Recruitment dynamics of ESCRT-III and Vps4 to endosomes and implications for reverse membrane budding" @default.
- W2764302512 cites W156225848 @default.
- W2764302512 cites W1579964499 @default.
- W2764302512 cites W1587896818 @default.
- W2764302512 cites W1834382122 @default.
- W2764302512 cites W1838684492 @default.
- W2764302512 cites W1964951207 @default.
- W2764302512 cites W1972465158 @default.
- W2764302512 cites W1973214920 @default.
- W2764302512 cites W1973743458 @default.
- W2764302512 cites W1981341553 @default.
- W2764302512 cites W1981515958 @default.
- W2764302512 cites W1987462880 @default.
- W2764302512 cites W1992721127 @default.
- W2764302512 cites W1993871755 @default.
- W2764302512 cites W1996987868 @default.
- W2764302512 cites W1998263911 @default.
- W2764302512 cites W2003609221 @default.
- W2764302512 cites W2003979167 @default.
- W2764302512 cites W2010542476 @default.
- W2764302512 cites W2011844788 @default.
- W2764302512 cites W2013947447 @default.
- W2764302512 cites W2018869629 @default.
- W2764302512 cites W2019215631 @default.
- W2764302512 cites W2022889543 @default.
- W2764302512 cites W2025029519 @default.
- W2764302512 cites W2029101220 @default.
- W2764302512 cites W2035178600 @default.
- W2764302512 cites W2040207361 @default.
- W2764302512 cites W2040909295 @default.
- W2764302512 cites W2042722403 @default.
- W2764302512 cites W2046068788 @default.
- W2764302512 cites W2053270078 @default.
- W2764302512 cites W2053957762 @default.
- W2764302512 cites W2064585995 @default.
- W2764302512 cites W2072238256 @default.
- W2764302512 cites W2073108127 @default.
- W2764302512 cites W2077154304 @default.
- W2764302512 cites W2078211289 @default.
- W2764302512 cites W2079319844 @default.
- W2764302512 cites W2079472849 @default.
- W2764302512 cites W2083087230 @default.
- W2764302512 cites W2083480759 @default.
- W2764302512 cites W2084641320 @default.
- W2764302512 cites W2093145534 @default.
- W2764302512 cites W2099674108 @default.
- W2764302512 cites W2101189847 @default.
- W2764302512 cites W2107885943 @default.
- W2764302512 cites W2115778080 @default.
- W2764302512 cites W2127943293 @default.
- W2764302512 cites W2129372332 @default.
- W2764302512 cites W2133554518 @default.
- W2764302512 cites W2134776091 @default.
- W2764302512 cites W2136590188 @default.
- W2764302512 cites W2137791756 @default.
- W2764302512 cites W2140279106 @default.
- W2764302512 cites W2142161929 @default.
- W2764302512 cites W2145494795 @default.
- W2764302512 cites W2145625720 @default.
- W2764302512 cites W2146279918 @default.
- W2764302512 cites W2153991959 @default.
- W2764302512 cites W2155277330 @default.
- W2764302512 cites W2155705945 @default.
- W2764302512 cites W2162773664 @default.
- W2764302512 cites W2166456692 @default.
- W2764302512 cites W2167531619 @default.
- W2764302512 cites W2168163553 @default.
- W2764302512 cites W2192401721 @default.
- W2764302512 cites W2194114568 @default.
- W2764302512 cites W2198297310 @default.
- W2764302512 cites W2294261229 @default.
- W2764302512 cites W2303653884 @default.
- W2764302512 cites W2311649981 @default.
- W2764302512 cites W2321038792 @default.
- W2764302512 cites W2338497250 @default.
- W2764302512 cites W2515641440 @default.
- W2764302512 cites W2522630538 @default.
- W2764302512 cites W2527818273 @default.
- W2764302512 cites W2605050533 @default.
- W2764302512 cites W2607322700 @default.