Matches in SemOpenAlex for { <https://semopenalex.org/work/W2765130109> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2765130109 abstract "BackgroundAs human embryonic stem cell (hESC) lines can be derived via multiple means, it is important to determine particular characteristics of individual lines that may dictate the applications to which they are best suited. The objective of this work was to determine points of equivalence and differences between conventionally-derived hESC and parthenote-derived hESC lines (phESC) in the undifferentiated state and during neural differentiation.Methodology/Principal FindingshESC and phESC were exposed to the same expansion conditions and subsequent neural and retinal pigmented epithelium (RPE) differentiation protocols. Growth rates and gross morphology were recorded during expansion. RTPCR for developmentally relevant genes and global DNA methylation profiling were used to compare gene expression and epigenetic characteristics. Parthenote lines proliferated more slowly than conventional hESC lines and yielded lower quantities of less mature differentiated cells in a neural progenitor cell (NPC) differentiation protocol. However, the cell lines performed similarly in a RPE differentiation protocol. The DNA methylation analysis showed similar general profiles, but the two cell types differed in methylation of imprinted genes. There were no major differences in gene expression between the lines before differentiation, but when differentiated into NPCs, the two cell types differed in expression of extracellular matrix (ECM) genes.Conclusions/SignificanceThese data show that hESC and phESC are similar in the undifferentiated state, and both cell types are capable of differentiation along neural lineages. The differences between the cell types, in proliferation and extent of differentiation, may be linked, in part, to the observed differences in ECM synthesis and methylation of imprinted genes." @default.
- W2765130109 created "2017-11-10" @default.
- W2765130109 creator A5015983327 @default.
- W2765130109 creator A5017876456 @default.
- W2765130109 creator A5023168270 @default.
- W2765130109 creator A5026283229 @default.
- W2765130109 creator A5058320505 @default.
- W2765130109 creator A5064602365 @default.
- W2765130109 creator A5065928472 @default.
- W2765130109 creator A5088320829 @default.
- W2765130109 creator A5088689985 @default.
- W2765130109 creator A5088893132 @default.
- W2765130109 date "2011-01-07" @default.
- W2765130109 modified "2023-09-22" @default.
- W2765130109 title "Equivalence of Conventionally-Derived and Parthenote-Derived Human Embryonic Stem Cells - eScholarship" @default.
- W2765130109 hasPublicationYear "2011" @default.
- W2765130109 type Work @default.
- W2765130109 sameAs 2765130109 @default.
- W2765130109 citedByCount "0" @default.
- W2765130109 crossrefType "journal-article" @default.
- W2765130109 hasAuthorship W2765130109A5015983327 @default.
- W2765130109 hasAuthorship W2765130109A5017876456 @default.
- W2765130109 hasAuthorship W2765130109A5023168270 @default.
- W2765130109 hasAuthorship W2765130109A5026283229 @default.
- W2765130109 hasAuthorship W2765130109A5058320505 @default.
- W2765130109 hasAuthorship W2765130109A5064602365 @default.
- W2765130109 hasAuthorship W2765130109A5065928472 @default.
- W2765130109 hasAuthorship W2765130109A5088320829 @default.
- W2765130109 hasAuthorship W2765130109A5088689985 @default.
- W2765130109 hasAuthorship W2765130109A5088893132 @default.
- W2765130109 hasConcept C104317684 @default.
- W2765130109 hasConcept C145103041 @default.
- W2765130109 hasConcept C148738053 @default.
- W2765130109 hasConcept C1491633281 @default.
- W2765130109 hasConcept C150194340 @default.
- W2765130109 hasConcept C153911025 @default.
- W2765130109 hasConcept C18431079 @default.
- W2765130109 hasConcept C189014844 @default.
- W2765130109 hasConcept C190727270 @default.
- W2765130109 hasConcept C201750760 @default.
- W2765130109 hasConcept C28328180 @default.
- W2765130109 hasConcept C54355233 @default.
- W2765130109 hasConcept C81885089 @default.
- W2765130109 hasConcept C86803240 @default.
- W2765130109 hasConcept C95444343 @default.
- W2765130109 hasConceptScore W2765130109C104317684 @default.
- W2765130109 hasConceptScore W2765130109C145103041 @default.
- W2765130109 hasConceptScore W2765130109C148738053 @default.
- W2765130109 hasConceptScore W2765130109C1491633281 @default.
- W2765130109 hasConceptScore W2765130109C150194340 @default.
- W2765130109 hasConceptScore W2765130109C153911025 @default.
- W2765130109 hasConceptScore W2765130109C18431079 @default.
- W2765130109 hasConceptScore W2765130109C189014844 @default.
- W2765130109 hasConceptScore W2765130109C190727270 @default.
- W2765130109 hasConceptScore W2765130109C201750760 @default.
- W2765130109 hasConceptScore W2765130109C28328180 @default.
- W2765130109 hasConceptScore W2765130109C54355233 @default.
- W2765130109 hasConceptScore W2765130109C81885089 @default.
- W2765130109 hasConceptScore W2765130109C86803240 @default.
- W2765130109 hasConceptScore W2765130109C95444343 @default.
- W2765130109 hasIssue "1" @default.
- W2765130109 hasLocation W27651301091 @default.
- W2765130109 hasOpenAccess W2765130109 @default.
- W2765130109 hasPrimaryLocation W27651301091 @default.
- W2765130109 hasRelatedWork W1964140992 @default.
- W2765130109 hasRelatedWork W1964499071 @default.
- W2765130109 hasRelatedWork W1971690182 @default.
- W2765130109 hasRelatedWork W1978912868 @default.
- W2765130109 hasRelatedWork W1980441010 @default.
- W2765130109 hasRelatedWork W1991838950 @default.
- W2765130109 hasRelatedWork W2005405046 @default.
- W2765130109 hasRelatedWork W2025818800 @default.
- W2765130109 hasRelatedWork W2027779389 @default.
- W2765130109 hasRelatedWork W2079340323 @default.
- W2765130109 hasRelatedWork W2101650913 @default.
- W2765130109 hasRelatedWork W2104360293 @default.
- W2765130109 hasRelatedWork W2148048540 @default.
- W2765130109 hasRelatedWork W2162183200 @default.
- W2765130109 hasRelatedWork W2520555029 @default.
- W2765130109 hasRelatedWork W2731969786 @default.
- W2765130109 hasRelatedWork W2761486548 @default.
- W2765130109 hasRelatedWork W2966830875 @default.
- W2765130109 hasRelatedWork W3022386644 @default.
- W2765130109 hasRelatedWork W3135607019 @default.
- W2765130109 hasVolume "6" @default.
- W2765130109 isParatext "false" @default.
- W2765130109 isRetracted "false" @default.
- W2765130109 magId "2765130109" @default.
- W2765130109 workType "article" @default.