Matches in SemOpenAlex for { <https://semopenalex.org/work/W2765182005> ?p ?o ?g. }
- W2765182005 endingPage "1700670" @default.
- W2765182005 startingPage "1700670" @default.
- W2765182005 abstract "Repair or regeneration of damaged nerves is still a challenging clinical task in reconstructive surgeries and regenerative medicine. Here, it is demonstrated that periodontal ligament stem cells (PDLSCs) and gingival mesenchymal stem cells (GMSCs) isolated from adult human periodontal and gingival tissues assume neuronal phenotype in vitro and in vivo via a subcutaneous transplantation model in nude mice. PDLSCs and GMSCs are encapsulated in a 3D scaffold based on alginate and hyaluronic acid hydrogels capable of sustained release of human nerve growth factor (NGF). The elasticity of the hydrogels affects the proliferation and differentiation of encapsulated MSCs within scaffolds. Moreover, it is observed that PDLSCs and GMSCs are stained positive for βIII-tubulin, while exhibiting high levels of gene expression related to neurogenic differentiation (βIII-tubulin and glial fibrillary acidic protein) via quantitative polymerase chain reaction (qPCR). Western blot analysis shows the importance of elasticity of the matrix and the presence of NGF in the neurogenic differentiation of encapsulated MSCs. In vivo, immunofluorescence staining for neurogenic specific protein markers confirms islands of dense positively stained structures inside transplanted hydrogels. As far as it is known, this study is the first demonstration of the application of PDLSCs and GMSCs as promising cell therapy candidates for nerve regeneration." @default.
- W2765182005 created "2017-11-10" @default.
- W2765182005 creator A5013876232 @default.
- W2765182005 creator A5014438006 @default.
- W2765182005 creator A5018129899 @default.
- W2765182005 creator A5057258281 @default.
- W2765182005 creator A5078195679 @default.
- W2765182005 creator A5078307926 @default.
- W2765182005 creator A5082068459 @default.
- W2765182005 date "2017-10-27" @default.
- W2765182005 modified "2023-10-01" @default.
- W2765182005 title "Human Periodontal Ligament- and Gingiva-derived Mesenchymal Stem Cells Promote Nerve Regeneration When Encapsulated in Alginate/Hyaluronic Acid 3D Scaffold" @default.
- W2765182005 cites W139260680 @default.
- W2765182005 cites W1485262942 @default.
- W2765182005 cites W1548290904 @default.
- W2765182005 cites W1796744353 @default.
- W2765182005 cites W1859089489 @default.
- W2765182005 cites W1913314522 @default.
- W2765182005 cites W1965425745 @default.
- W2765182005 cites W1965832071 @default.
- W2765182005 cites W1971500643 @default.
- W2765182005 cites W1974010229 @default.
- W2765182005 cites W1974973994 @default.
- W2765182005 cites W1980867815 @default.
- W2765182005 cites W1999490106 @default.
- W2765182005 cites W2005848913 @default.
- W2765182005 cites W2007260979 @default.
- W2765182005 cites W2008737522 @default.
- W2765182005 cites W2014498232 @default.
- W2765182005 cites W2021034040 @default.
- W2765182005 cites W2022300394 @default.
- W2765182005 cites W2025371271 @default.
- W2765182005 cites W2026757718 @default.
- W2765182005 cites W2027654288 @default.
- W2765182005 cites W2033986003 @default.
- W2765182005 cites W2036452348 @default.
- W2765182005 cites W2038708423 @default.
- W2765182005 cites W2044959577 @default.
- W2765182005 cites W2047866615 @default.
- W2765182005 cites W2049740710 @default.
- W2765182005 cites W2051476941 @default.
- W2765182005 cites W2051766546 @default.
- W2765182005 cites W2057628483 @default.
- W2765182005 cites W2060498128 @default.
- W2765182005 cites W2066064602 @default.
- W2765182005 cites W2067161293 @default.
- W2765182005 cites W2068080217 @default.
- W2765182005 cites W2077398414 @default.
- W2765182005 cites W2078730186 @default.
- W2765182005 cites W2079356804 @default.
- W2765182005 cites W2083663270 @default.
- W2765182005 cites W2084610464 @default.
- W2765182005 cites W2085831434 @default.
- W2765182005 cites W2093229003 @default.
- W2765182005 cites W2098737512 @default.
- W2765182005 cites W2102726602 @default.
- W2765182005 cites W2106301042 @default.
- W2765182005 cites W2106413293 @default.
- W2765182005 cites W2116864112 @default.
- W2765182005 cites W2122838755 @default.
- W2765182005 cites W2136767600 @default.
- W2765182005 cites W2143326638 @default.
- W2765182005 cites W2144224215 @default.
- W2765182005 cites W2148562877 @default.
- W2765182005 cites W2149709195 @default.
- W2765182005 cites W2158016927 @default.
- W2765182005 cites W2158837806 @default.
- W2765182005 cites W2165680331 @default.
- W2765182005 cites W2262163452 @default.
- W2765182005 cites W2270498673 @default.
- W2765182005 cites W2309132905 @default.
- W2765182005 cites W2320375598 @default.
- W2765182005 cites W2323729311 @default.
- W2765182005 cites W2331103940 @default.
- W2765182005 cites W2613217394 @default.
- W2765182005 cites W30954308 @default.
- W2765182005 doi "https://doi.org/10.1002/adhm.201700670" @default.
- W2765182005 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5813692" @default.
- W2765182005 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29076281" @default.
- W2765182005 hasPublicationYear "2017" @default.
- W2765182005 type Work @default.
- W2765182005 sameAs 2765182005 @default.
- W2765182005 citedByCount "58" @default.
- W2765182005 countsByYear W27651820052018 @default.
- W2765182005 countsByYear W27651820052019 @default.
- W2765182005 countsByYear W27651820052020 @default.
- W2765182005 countsByYear W27651820052021 @default.
- W2765182005 countsByYear W27651820052022 @default.
- W2765182005 countsByYear W27651820052023 @default.
- W2765182005 crossrefType "journal-article" @default.
- W2765182005 hasAuthorship W2765182005A5013876232 @default.
- W2765182005 hasAuthorship W2765182005A5014438006 @default.
- W2765182005 hasAuthorship W2765182005A5018129899 @default.
- W2765182005 hasAuthorship W2765182005A5057258281 @default.
- W2765182005 hasAuthorship W2765182005A5078195679 @default.
- W2765182005 hasAuthorship W2765182005A5078307926 @default.
- W2765182005 hasAuthorship W2765182005A5082068459 @default.
- W2765182005 hasBestOaLocation W27651820052 @default.