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- W2765231623 abstract "Nearly all eukaryotic messenger RNA precursors must undergo cleavage and polyadenylation at their 3′-end for maturation. A crucial step in this process is the recognition of the AAUAAA polyadenylation signal (PAS), and the molecular mechanism of this recognition has been a long-standing problem. Here, we report the cryo-electron microscopy structure of a quaternary complex of human CPSF-160, WDR33, CPSF-30, and an AAUAAA RNA at 3.4-Å resolution. Strikingly, the AAUAAA PAS assumes an unusual conformation that allows this short motif to be bound directly by both CPSF-30 and WDR33. The A1 and A2 bases are recognized specifically by zinc finger 2 (ZF2) of CPSF-30 and the A4 and A5 bases by ZF3. Interestingly, the U3 and A6 bases form an intramolecular Hoogsteen base pair and directly contact WDR33. CPSF-160 functions as an essential scaffold and preorganizes CPSF-30 and WDR33 for high-affinity binding to AAUAAA. Our findings provide an elegant molecular explanation for how PAS sequences are recognized for mRNA 3′-end formation." @default.
- W2765231623 created "2017-11-10" @default.
- W2765231623 creator A5004904484 @default.
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- W2765231623 date "2017-12-05" @default.
- W2765231623 modified "2023-10-05" @default.
- W2765231623 title "Molecular basis for the recognition of the human AAUAAA polyadenylation signal" @default.
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- W2765231623 cites W1828587713 @default.
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- W2765231623 cites W1978501040 @default.
- W2765231623 cites W1979417055 @default.
- W2765231623 cites W1985267020 @default.
- W2765231623 cites W1985585046 @default.
- W2765231623 cites W1985629904 @default.
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- W2765231623 cites W1991297120 @default.
- W2765231623 cites W2000750967 @default.
- W2765231623 cites W2003805923 @default.
- W2765231623 cites W2006337626 @default.
- W2765231623 cites W2010088308 @default.
- W2765231623 cites W2020204531 @default.
- W2765231623 cites W2026111998 @default.
- W2765231623 cites W2037312364 @default.
- W2765231623 cites W2038424676 @default.
- W2765231623 cites W2043111511 @default.
- W2765231623 cites W2044648543 @default.
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- W2765231623 cites W2064075969 @default.
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- W2765231623 cites W2085791091 @default.
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- W2765231623 cites W2105545925 @default.
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- W2765231623 cites W2108906526 @default.
- W2765231623 cites W2109184569 @default.
- W2765231623 cites W2109235554 @default.
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- W2765231623 cites W2115612309 @default.
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- W2765231623 cites W2159666069 @default.
- W2765231623 cites W2162816788 @default.
- W2765231623 cites W2165518330 @default.
- W2765231623 cites W2166494447 @default.
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- W2765231623 cites W2342500021 @default.
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- W2765231623 doi "https://doi.org/10.1073/pnas.1718723115" @default.
- W2765231623 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5816196" @default.
- W2765231623 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29208711" @default.
- W2765231623 hasPublicationYear "2017" @default.
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