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- W276530844 abstract "Three sets of primers specific for selected Verticillium dahliae genome fragments were tested for their suitability for detection of this pathogen in infected plants and soil. Using primers VMSP1/VMSP2 and VDS1/VDS2, detection of fungus DNA was possible only for single samples derived from leaves of inoculated plants. Primers possessing targeted sites internal to a sequence of product obtained in reaction with DV1/DV2 primers allowed generating specific products on the DNA templates isolated from all inoculated plants. Application of the same nested-PCR primers was partially successful in detecting pathogen in the soil. PCR product with size of 320 bp was obtained for 7 of 10 soil samples, for which the presence of fungus was confirmed earlier with biological method." @default.
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- W276530844 date "2008-01-01" @default.
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- W276530844 title "THE SUITABILITY OF PCR-BASED TECHNIQUES FOR DETECTING Verticillium dahliae IN STRAWBERRY PLANTS AND SOIL" @default.
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