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- W2765432991 endingPage "602" @default.
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- W2765432991 abstract "Liver metastasis of colorectal cancer (CRC) remains high mortality and the mechanism is still unknown. Here we investigated the effects of inhibitor of DNA binding 2 (Id2) on growth and liver metastasis of CRC.qPCR and western blotting were used to demonstrate mRNA and protein expressions in Id2-knockdown HCT116 cells. Cell growth was observed by cell proliferation assay, colony formation assay and flow cytometry. Cell migration and invasion were observed with wound healing assay and transwell migration and invasion assay. The effects of Id2 knockdown on tumor growth and liver metastasis in vivo were evaluated respectively with subcutaneous tumor model and colorectal liver metastasis model by injecting HCT116 cells into the mesentery triangle of cecum in mice.Id2 overexpression was found in CRC cell lines. Id2 knockdown resulted in a reduction in the proliferation, colony formation, migration and invasion of HCT116 cells. The suppression of cell proliferation was accompanied by the cell cycle arrest in the G0/G1 phase with down-regulation of Cyclin D1, Cyclin E, p-Cdk2/3, Cdk6, p-p27 and up-regulation of p21 and p27. Id2 knockdown reversed epithelial-mesenchymal transition (EMT) through increasing E-Cadherin and inhibiting N-Cadherin, Vimentin, β-catenin, Snail and Slug. Id2 was also found to inhibit CRC metastasis via MMP2, MMP9 and TIMP-1. Furthermore, Id2 knockdown suppressed CRC liver metastasis in vivo.Id2 promotes CRC growth through activation of the PI3K/AKT signaling pathway, and triggers EMT to enhance CRC migration and invasion." @default.
- W2765432991 created "2017-11-10" @default.
- W2765432991 creator A5015348605 @default.
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- W2765432991 creator A5054803465 @default.
- W2765432991 creator A5057140415 @default.
- W2765432991 creator A5060810000 @default.
- W2765432991 creator A5065341052 @default.
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- W2765432991 creator A5079913663 @default.
- W2765432991 creator A5081203691 @default.
- W2765432991 creator A5089886054 @default.
- W2765432991 date "2017-11-01" @default.
- W2765432991 modified "2023-10-04" @default.
- W2765432991 title "Update on current problems in colorectal liver metastasis" @default.
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