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• W2765679375 abstract "Many of mRNAs synthesized during pollen development are translated after germination, and we hypothesize that they are stored in cytoplasmic granules. We analyzed the cellular localization of the SKS14 and AT59 Arabidopsis mRNAs, which are orthologues of the tobacco NTP303 and tomato LAT59 pollen mRNAs, respectively, by artificially labeling the transcripts with a MS2-GFP chimera. A MATLAB-automated image analysis helped to identify the presence of cytoplasmic SKS14 and AT59 mRNA granules in mature pollen grains. These mRNA granules partially colocalized with VCS and DCP1, two processing body (PB) proteins. Finally, we found a temporal correlation between SKS14 protein accumulation and the disappearance of SKS14 mRNA granules during pollen germination. These results contribute to unveil a mechanism for translational regulation in Arabidopsis thaliana pollen." @default.
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• W2765679375 date "2017-10-01" @default.
• W2765679375 modified "2023-10-16" @default.
• W2765679375 title "Two Arabidopsis late pollen transcripts are detected in cytoplasmic granules" @default.
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• W2765679375 doi "https://doi.org/10.1002/pld3.12" @default.
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