Matches in SemOpenAlex for { <https://semopenalex.org/work/W2765694865> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2765694865 startingPage "100" @default.
- W2765694865 abstract "Rationale. Human amniotic fluid-derived (hAF) stem cells are considered a novel class of multipotent stem cells, sharing characteristics of both embryonic and adult stem cells. In fact, they proliferate rapidly, are able to differentiate into cells of all the embryonic germ layers, but do not form teratoma. It has been already reported that the embryoid bodies (EBs) obtained from hAFs have a cardiac potential, but it has not been described a functional terminal differentiation in cardiomyocytes (CMs) yet. Objective. Aim of this study was to foster the cardiomyogenic potential of hAFs in order to obtain a cellular population with morphological and functional features of CMs. Methods and Results. AFCs were exposed sequentially to inducing factors (Ascorbic Acid, 5-Azacytidine, BMP4, ActivinA, VEGF) up to 15 days and differentiation was monitored. Only the hAF samples expressing the multipotency markers SSEA4, OCT4 and CD90 (CardiopoieticAF) responded to the differentiation process loosing their stemness and increasing the cardiac nuclear factors NKX2.5 and GATA4. After the differentiation cells expressed high levels of the sarcomeric proteins (cTnT, αMHC and αSA), the gap junction marker Connexin43 and both atrial and ventricular markers; moreover, up to 90% of the cells was positive for CACNA1C and SERCA2a, cardiac calcium pumps involved in the excitation/contraction coupling, and about 30% of the CardiopoieticAF-derived cells presented spontaneous intracellular Ca2+ waves and Ca2+ fluctuation in response to caffeine or adrenergic stimulation. Some spontaneous beating foci were also observed. Conclusion. Our results demonstrate that CardiopoietichAFs can fully differentiate into a homogenous population of CM-like cells, characterized by cardiac-specific molecular, structural, and functional properties. Thus, CardiopoietichAFs can hold great promise for the development of in vitro models of cardiac genetic disorders, for drug discovery and testing, and for the emerging field of cardiovascular regenerative medicine." @default.
- W2765694865 created "2017-11-10" @default.
- W2765694865 creator A5019632447 @default.
- W2765694865 creator A5036075776 @default.
- W2765694865 creator A5036376628 @default.
- W2765694865 creator A5037995826 @default.
- W2765694865 creator A5068820892 @default.
- W2765694865 creator A5069050613 @default.
- W2765694865 creator A5078032532 @default.
- W2765694865 creator A5080022328 @default.
- W2765694865 date "2017-01-01" @default.
- W2765694865 modified "2023-09-24" @default.
- W2765694865 title "Human Cardiopoietic Amniotic Fluid cell population: characterization and terminal differentiation" @default.
- W2765694865 doi "https://doi.org/10.13128/ijae-21492" @default.
- W2765694865 hasPublicationYear "2017" @default.
- W2765694865 type Work @default.
- W2765694865 sameAs 2765694865 @default.
- W2765694865 citedByCount "0" @default.
- W2765694865 crossrefType "journal-article" @default.
- W2765694865 hasAuthorship W2765694865A5019632447 @default.
- W2765694865 hasAuthorship W2765694865A5036075776 @default.
- W2765694865 hasAuthorship W2765694865A5036376628 @default.
- W2765694865 hasAuthorship W2765694865A5037995826 @default.
- W2765694865 hasAuthorship W2765694865A5068820892 @default.
- W2765694865 hasAuthorship W2765694865A5069050613 @default.
- W2765694865 hasAuthorship W2765694865A5078032532 @default.
- W2765694865 hasAuthorship W2765694865A5080022328 @default.
- W2765694865 hasConcept C104317684 @default.
- W2765694865 hasConcept C107459253 @default.
- W2765694865 hasConcept C126322002 @default.
- W2765694865 hasConcept C143948831 @default.
- W2765694865 hasConcept C145103041 @default.
- W2765694865 hasConcept C148738053 @default.
- W2765694865 hasConcept C185592680 @default.
- W2765694865 hasConcept C203014093 @default.
- W2765694865 hasConcept C26805974 @default.
- W2765694865 hasConcept C2778532138 @default.
- W2765694865 hasConcept C28328180 @default.
- W2765694865 hasConcept C2908647359 @default.
- W2765694865 hasConcept C36036425 @default.
- W2765694865 hasConcept C500558357 @default.
- W2765694865 hasConcept C55493867 @default.
- W2765694865 hasConcept C71924100 @default.
- W2765694865 hasConcept C86803240 @default.
- W2765694865 hasConcept C95444343 @default.
- W2765694865 hasConcept C99454951 @default.
- W2765694865 hasConceptScore W2765694865C104317684 @default.
- W2765694865 hasConceptScore W2765694865C107459253 @default.
- W2765694865 hasConceptScore W2765694865C126322002 @default.
- W2765694865 hasConceptScore W2765694865C143948831 @default.
- W2765694865 hasConceptScore W2765694865C145103041 @default.
- W2765694865 hasConceptScore W2765694865C148738053 @default.
- W2765694865 hasConceptScore W2765694865C185592680 @default.
- W2765694865 hasConceptScore W2765694865C203014093 @default.
- W2765694865 hasConceptScore W2765694865C26805974 @default.
- W2765694865 hasConceptScore W2765694865C2778532138 @default.
- W2765694865 hasConceptScore W2765694865C28328180 @default.
- W2765694865 hasConceptScore W2765694865C2908647359 @default.
- W2765694865 hasConceptScore W2765694865C36036425 @default.
- W2765694865 hasConceptScore W2765694865C500558357 @default.
- W2765694865 hasConceptScore W2765694865C55493867 @default.
- W2765694865 hasConceptScore W2765694865C71924100 @default.
- W2765694865 hasConceptScore W2765694865C86803240 @default.
- W2765694865 hasConceptScore W2765694865C95444343 @default.
- W2765694865 hasConceptScore W2765694865C99454951 @default.
- W2765694865 hasIssue "1" @default.
- W2765694865 hasLocation W27656948651 @default.
- W2765694865 hasOpenAccess W2765694865 @default.
- W2765694865 hasPrimaryLocation W27656948651 @default.
- W2765694865 hasRelatedWork W1975410103 @default.
- W2765694865 hasRelatedWork W2011844821 @default.
- W2765694865 hasRelatedWork W2015243651 @default.
- W2765694865 hasRelatedWork W2050688392 @default.
- W2765694865 hasRelatedWork W2051874051 @default.
- W2765694865 hasRelatedWork W2061910157 @default.
- W2765694865 hasRelatedWork W2063877672 @default.
- W2765694865 hasRelatedWork W2064507869 @default.
- W2765694865 hasRelatedWork W2070017026 @default.
- W2765694865 hasRelatedWork W2076117584 @default.
- W2765694865 hasRelatedWork W208427836 @default.
- W2765694865 hasRelatedWork W2100092472 @default.
- W2765694865 hasRelatedWork W2128085444 @default.
- W2765694865 hasRelatedWork W2314836909 @default.
- W2765694865 hasRelatedWork W2612791760 @default.
- W2765694865 hasRelatedWork W2765128519 @default.
- W2765694865 hasRelatedWork W2787293269 @default.
- W2765694865 hasRelatedWork W289774157 @default.
- W2765694865 hasRelatedWork W2913639567 @default.
- W2765694865 hasRelatedWork W80209216 @default.
- W2765694865 hasVolume "122" @default.
- W2765694865 isParatext "false" @default.
- W2765694865 isRetracted "false" @default.
- W2765694865 magId "2765694865" @default.
- W2765694865 workType "article" @default.