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- W2765797687 abstract "Publisher Summary This chapter presents a guinea pig model of herpesvirus genital infection. Symptomatic genital infection can be produced in either inbred or outbred female animals but outbred female Hartley guinea pigs of 250–350 g are typically used. No specialized housing, care nor pretreatment is required in this model. Viral inocula can be prepared on any susceptible cell monolayer, and so it is not necessary to grow the virus in guinea pig cells. Virus stocks are titrated, aliquoted, and stored frozen at -80˚ C, and then they are quickly thawed, diluted to the appropriate titer, and kept on ice until they are used. Both HSV-1 and HSV-2 strains have been used in genital herpes studies in guinea pigs. Both virus types produce comparable primary infections. However, nearly all HSV-2-infected animals subsequently manifest recurrent disease with recurrences developing frequently, while many HSV-1-infected animals experience no recurrent disease and those that do have only infrequent recurrences. In general, HSV-2 strain MS and strain 333 both cause reproducible primary and recurrent infections and are widely used for the testing of antiviral drugs. Prior to virus inoculation, the vaginal closure membrane is ruptured by using a moistened calcium alginate-tipped swab. The viral inoculum is instilled into the vagina in a volume of 0.1–0.2 ml using a 1 cc tuberculin syringe and plastic catheter or a micropipettor. To optimize the number of animals that become infected, virus instillation can be performed twice within a short time interval. The magnitude and duration of acute virus replication in the genital tract can be quantified by plaque titration of vaginal swab samples. Peak virus titers are typically seen on day 1 or 2 post-inoculation (p.i.) and then gradually decline until approximately day 10 p.i." @default.
- W2765797687 created "2017-11-10" @default.
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- W2765797687 date "1999-01-01" @default.
- W2765797687 modified "2023-10-16" @default.
- W2765797687 title "Animal Models of Herpesvirus Genital Infection" @default.
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- W2765797687 doi "https://doi.org/10.1016/b978-012775390-4/50247-5" @default.
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