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- W2765861384 abstract "Abstract Using a comprehensive library of histone H2A and H2B mutants, we assessed the biological function of each amino acid residue involved in various stress conditions including exposure to different DNA damage-inducing reagents, different growth temperatures, and other chemicals. H2B N- and H2A C-termini were critical for maintaining nucleosome function and mutations in these regions led to pleiotropic phenotypes. Additionally, two screens were performed using this library, monitoring heterochromatin gene silencing and genome stability, to identify residues that could compromise normal function when mutated. Many distinctive regions within the nucleosome were revealed. Furthermore, we used the barcode sequencing (bar-seq) method to profile the mutant composition of many libraries in one high-throughput sequencing experiment, greatly reducing the labor and increasing the capacity. This study not only demonstrates the applications of the versatile histone library, but also reveals many previously unknown functions of histone H2A and H2B." @default.
- W2765861384 created "2017-11-10" @default.
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- W2765861384 date "2017-12-01" @default.
- W2765861384 modified "2023-10-16" @default.
- W2765861384 title "Dissecting Nucleosome Function with a Comprehensive Histone H2A and H2B Mutant Library" @default.
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- W2765861384 cites W1987302558 @default.
- W2765861384 cites W1989092936 @default.
- W2765861384 cites W1992213480 @default.
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- W2765861384 cites W2139384923 @default.
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- W2765861384 cites W2144792857 @default.
- W2765861384 cites W2150609732 @default.
- W2765861384 cites W2150673680 @default.
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- W2765861384 doi "https://doi.org/10.1534/g3.117.300252" @default.
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- W2765861384 hasPublicationYear "2017" @default.
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