FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2765984570> ?p ?o ?g. }

• W2765984570 endingPage "371" @default.
• W2765984570 startingPage "362" @default.
• W2765984570 abstract "β-Glucosidase plays an important role in the degradation of cellulose. In this study, a novel β-glucosidase ccbgl1b gene for a glycosyl hydrolase (GH) family 1 enzyme was cloned from the genome of Cellulosimicrobium cellulans and expressed in Escherichia coli BL21 cells. The sequence contained an open reading frame of 1494 bp, encoded a polypeptide of 497 amino acid residues. The recombinant protein CcBgl1B was purified by Ni sepharose fastflow affinity chromatography and had a molecular weight of 57 kDa, as judged by SDS-PAGE. The optimum β-glucosidase activity was observed at 55 °C and pH 6.0. Recombinant CcBgl1B was found to be most active against aryl-glycosides p-nitrophenyl-β-D-glucopyranoside (pNPβGlc), followed by p-nitrophenyl-β-D-galactopyranoside (pNPβGal). Using disaccharides as substrates, the enzyme efficiently cleaved β-linked glucosyl-disaccharides, including sophorose (β-1,2-), laminaribiose (β-1,3-) and cellobiose (β-1,4-). In addition, a range of cello-oligosaccharides including cellotriose, cellotetraose and cellopentaose were hydrolysed by CcBgl1B to produce glucose. The interaction mode between the enzyme and the substrates driving the reaction was modelled using a molecular docking approach. Understanding how the GH1 enzyme CcBgl1B from C. cellulans works, particularly its activity against cello-oligosaccharides, would be potentially useful for biotechnological applications of cellulose degradation." @default.
• W2765984570 created "2017-11-10" @default.
• W2765984570 creator A5004241428 @default.
• W2765984570 creator A5005071062 @default.
• W2765984570 creator A5008292444 @default.
• W2765984570 creator A5008943595 @default.
• W2765984570 creator A5044000967 @default.
• W2765984570 creator A5051707361 @default.
• W2765984570 creator A5056591137 @default.
• W2765984570 creator A5088757887 @default.
• W2765984570 date "2017-10-26" @default.
• W2765984570 modified "2023-10-17" @default.
• W2765984570 title "Cloning, expression and biochemical characterization of a GH1 β-glucosidase from <i>Cellulosimicrobium cellulans</i>" @default.
• W2765984570 cites W1139914957 @default.
• W2765984570 cites W1552110160 @default.
• W2765984570 cites W1572118888 @default.
• W2765984570 cites W1967789813 @default.
• W2765984570 cites W1969895051 @default.
• W2765984570 cites W1971670497 @default.
• W2765984570 cites W1981009721 @default.
• W2765984570 cites W1995253178 @default.
• W2765984570 cites W2009674410 @default.
• W2765984570 cites W2018931215 @default.
• W2765984570 cites W2031940323 @default.
• W2765984570 cites W2043174582 @default.
• W2765984570 cites W2048930689 @default.
• W2765984570 cites W2049139172 @default.
• W2765984570 cites W2059033374 @default.
• W2765984570 cites W2082234168 @default.
• W2765984570 cites W2086538274 @default.
• W2765984570 cites W2087765717 @default.
• W2765984570 cites W2107769173 @default.
• W2765984570 cites W2108929776 @default.
• W2765984570 cites W2109093244 @default.
• W2765984570 cites W2120895770 @default.
• W2765984570 cites W2127753803 @default.
• W2765984570 cites W2134741857 @default.
• W2765984570 cites W2173382132 @default.
• W2765984570 cites W2228653419 @default.
• W2765984570 cites W2285036593 @default.
• W2765984570 cites W2336176762 @default.
• W2765984570 cites W2345457103 @default.
• W2765984570 cites W2418879058 @default.
• W2765984570 cites W2419653649 @default.
• W2765984570 cites W2492346507 @default.
• W2765984570 cites W2513014227 @default.
• W2765984570 cites W2563192597 @default.
• W2765984570 cites W2740348174 @default.
• W2765984570 cites W776562372 @default.
• W2765984570 doi "https://doi.org/10.1080/10242422.2017.1395415" @default.
• W2765984570 hasPublicationYear "2017" @default.
• W2765984570 type Work @default.
• W2765984570 sameAs 2765984570 @default.
• W2765984570 citedByCount "7" @default.
• W2765984570 countsByYear W27659845702019 @default.
• W2765984570 countsByYear W27659845702021 @default.
• W2765984570 countsByYear W27659845702023 @default.
• W2765984570 crossrefType "journal-article" @default.
• W2765984570 hasAuthorship W2765984570A5004241428 @default.
• W2765984570 hasAuthorship W2765984570A5005071062 @default.
• W2765984570 hasAuthorship W2765984570A5008292444 @default.
• W2765984570 hasAuthorship W2765984570A5008943595 @default.
• W2765984570 hasAuthorship W2765984570A5044000967 @default.
• W2765984570 hasAuthorship W2765984570A5051707361 @default.
• W2765984570 hasAuthorship W2765984570A5056591137 @default.
• W2765984570 hasAuthorship W2765984570A5088757887 @default.
• W2765984570 hasConcept C104317684 @default.
• W2765984570 hasConcept C141603559 @default.
• W2765984570 hasConcept C179247698 @default.
• W2765984570 hasConcept C181199279 @default.
• W2765984570 hasConcept C185592680 @default.
• W2765984570 hasConcept C196032511 @default.
• W2765984570 hasConcept C2780120296 @default.
• W2765984570 hasConcept C2780412358 @default.
• W2765984570 hasConcept C2780906977 @default.
• W2765984570 hasConcept C40767141 @default.
• W2765984570 hasConcept C547475151 @default.
• W2765984570 hasConcept C55493867 @default.
• W2765984570 hasConcept C94412978 @default.
• W2765984570 hasConceptScore W2765984570C104317684 @default.
• W2765984570 hasConceptScore W2765984570C141603559 @default.
• W2765984570 hasConceptScore W2765984570C179247698 @default.
• W2765984570 hasConceptScore W2765984570C181199279 @default.
• W2765984570 hasConceptScore W2765984570C185592680 @default.
• W2765984570 hasConceptScore W2765984570C196032511 @default.
• W2765984570 hasConceptScore W2765984570C2780120296 @default.
• W2765984570 hasConceptScore W2765984570C2780412358 @default.
• W2765984570 hasConceptScore W2765984570C2780906977 @default.
• W2765984570 hasConceptScore W2765984570C40767141 @default.
• W2765984570 hasConceptScore W2765984570C547475151 @default.
• W2765984570 hasConceptScore W2765984570C55493867 @default.
• W2765984570 hasConceptScore W2765984570C94412978 @default.
• W2765984570 hasFunder F4320321001 @default.
• W2765984570 hasIssue "5" @default.
• W2765984570 hasLocation W27659845701 @default.
• W2765984570 hasOpenAccess W2765984570 @default.
• W2765984570 hasPrimaryLocation W27659845701 @default.
• W2765984570 hasRelatedWork W1778200108 @default.

• next