Matches in SemOpenAlex for { <https://semopenalex.org/work/W2766228701> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2766228701 abstract "We hypothesize that DNA damage induced by high local energy deposition, occurring when cells are traversed by high-LET (Linear Energy Transfer) particles, can be experimentally modeled by exposing cells to high doses of low-LET. In this work, we validate such hypothesis by characterizing and correlating the time dependence of 53BP1 radiation-induced foci (RIF) for various doses and LET across 72 primary skin fibroblast from mice. This genetically diverse population allows us to understand how genetic may modulate the dose and LET relationship. The cohort was made on average from 3 males and 3 females belonging to 15 different strains of mice with various genetic backgrounds, including the collaborative cross (CC) genetic model (10 strains) and 5 reference mice strains. Cells were exposed to two fluences of three HZE (High Atomic Energy) particles (Si 350 megaelectronvolts per nucleon, Ar 350 megaelectronvolts per nucleon and Fe 600 megaelectronvolts per nucleon) and to 0.1, 1 and 4 grays from a 160 kilovolt X-ray. Individual radiation sensitivity was investigated by high throughput measurements of DNA repair kinetics for different doses of each radiation type. The 53BP1 RIF dose response to high-LET particles showed a linear dependency that matched the expected number of tracks per cell, clearly illustrating the fact that close-by DNA double strand breaks along tracks cluster within one single RIF. By comparing the slope of the high-LET dose curve to the expected number of tracks per cell we computed the number of remaining unrepaired tracks as a function of time post-irradiation. Results show that the percentage of unrepaired track over a 48 hours follow-up is higher as the LET increases across all strains. We also observe a strong correlation between the high dose repair kinetics following exposure to 160 kilovolts X-ray and the repair kinetics of high-LET tracks, with higher correlation with higher LET. At the in-vivo level for the 10-CC strains, we observe that drops in the number of T-cells and B-cells found in the blood of mice 24 hours after exposure to 0.1 gray of 320 kilovolts X-ray correlate well with slower DNA repair kinetics in skin cells exposed to X-ray. Overall, our results suggest that repair kinetics found in skin is a surrogate marker for in-vivo radiation sensitivity in other tissue, such as blood cells, and that such response is modulated by genetic variability." @default.
- W2766228701 created "2017-11-10" @default.
- W2766228701 creator A5008434101 @default.
- W2766228701 creator A5008813757 @default.
- W2766228701 creator A5031216179 @default.
- W2766228701 creator A5041861945 @default.
- W2766228701 creator A5061738677 @default.
- W2766228701 creator A5063985740 @default.
- W2766228701 creator A5075368818 @default.
- W2766228701 creator A5076358770 @default.
- W2766228701 creator A5082441764 @default.
- W2766228701 date "2017-10-15" @default.
- W2766228701 modified "2023-10-03" @default.
- W2766228701 title "Genetic Correlation with the DNA Repair Assay in Mice Exposed to High-LET" @default.
- W2766228701 hasPublicationYear "2017" @default.
- W2766228701 type Work @default.
- W2766228701 sameAs 2766228701 @default.
- W2766228701 citedByCount "0" @default.
- W2766228701 crossrefType "journal-article" @default.
- W2766228701 hasAuthorship W2766228701A5008434101 @default.
- W2766228701 hasAuthorship W2766228701A5008813757 @default.
- W2766228701 hasAuthorship W2766228701A5031216179 @default.
- W2766228701 hasAuthorship W2766228701A5041861945 @default.
- W2766228701 hasAuthorship W2766228701A5061738677 @default.
- W2766228701 hasAuthorship W2766228701A5063985740 @default.
- W2766228701 hasAuthorship W2766228701A5075368818 @default.
- W2766228701 hasAuthorship W2766228701A5076358770 @default.
- W2766228701 hasAuthorship W2766228701A5082441764 @default.
- W2766228701 hasConcept C111337013 @default.
- W2766228701 hasConcept C11928243 @default.
- W2766228701 hasConcept C121332964 @default.
- W2766228701 hasConcept C12554922 @default.
- W2766228701 hasConcept C134935766 @default.
- W2766228701 hasConcept C143425029 @default.
- W2766228701 hasConcept C153385146 @default.
- W2766228701 hasConcept C153911025 @default.
- W2766228701 hasConcept C185544564 @default.
- W2766228701 hasConcept C2908647359 @default.
- W2766228701 hasConcept C2989005 @default.
- W2766228701 hasConcept C54355233 @default.
- W2766228701 hasConcept C552990157 @default.
- W2766228701 hasConcept C71924100 @default.
- W2766228701 hasConcept C86611320 @default.
- W2766228701 hasConcept C86803240 @default.
- W2766228701 hasConcept C99454951 @default.
- W2766228701 hasConceptScore W2766228701C111337013 @default.
- W2766228701 hasConceptScore W2766228701C11928243 @default.
- W2766228701 hasConceptScore W2766228701C121332964 @default.
- W2766228701 hasConceptScore W2766228701C12554922 @default.
- W2766228701 hasConceptScore W2766228701C134935766 @default.
- W2766228701 hasConceptScore W2766228701C143425029 @default.
- W2766228701 hasConceptScore W2766228701C153385146 @default.
- W2766228701 hasConceptScore W2766228701C153911025 @default.
- W2766228701 hasConceptScore W2766228701C185544564 @default.
- W2766228701 hasConceptScore W2766228701C2908647359 @default.
- W2766228701 hasConceptScore W2766228701C2989005 @default.
- W2766228701 hasConceptScore W2766228701C54355233 @default.
- W2766228701 hasConceptScore W2766228701C552990157 @default.
- W2766228701 hasConceptScore W2766228701C71924100 @default.
- W2766228701 hasConceptScore W2766228701C86611320 @default.
- W2766228701 hasConceptScore W2766228701C86803240 @default.
- W2766228701 hasConceptScore W2766228701C99454951 @default.
- W2766228701 hasOpenAccess W2766228701 @default.
- W2766228701 hasRelatedWork W1970963964 @default.
- W2766228701 hasRelatedWork W2005609716 @default.
- W2766228701 hasRelatedWork W2010651307 @default.
- W2766228701 hasRelatedWork W2056770903 @default.
- W2766228701 hasRelatedWork W2103461789 @default.
- W2766228701 hasRelatedWork W2160608614 @default.
- W2766228701 hasRelatedWork W2164811914 @default.
- W2766228701 hasRelatedWork W2333440328 @default.
- W2766228701 hasRelatedWork W2338350735 @default.
- W2766228701 hasRelatedWork W2398626843 @default.
- W2766228701 hasRelatedWork W2511265374 @default.
- W2766228701 hasRelatedWork W2724614972 @default.
- W2766228701 hasRelatedWork W2789533218 @default.
- W2766228701 hasRelatedWork W3020848387 @default.
- W2766228701 hasRelatedWork W3142660488 @default.
- W2766228701 hasRelatedWork W3166153742 @default.
- W2766228701 hasRelatedWork W3167646383 @default.
- W2766228701 hasRelatedWork W3173181207 @default.
- W2766228701 hasRelatedWork W3176175011 @default.
- W2766228701 hasRelatedWork W840770664 @default.
- W2766228701 isParatext "false" @default.
- W2766228701 isRetracted "false" @default.
- W2766228701 magId "2766228701" @default.
- W2766228701 workType "article" @default.