Matches in SemOpenAlex for { <https://semopenalex.org/work/W2766561570> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2766561570 abstract "Ferritin heavy polypeptide 1 (FTH1) plays a pivotal role in response to viral infections. FTH1 expression is modulated by various pathogens, but the regulatory mechanisms are unknown. We firstly construct duck hepatitis virus 1 (DHV-1) infection model, including morbid ducklings, non-morbid ducklings and control ducklings. Then the mRNA expression of duck FTH1 (duFTH1) was measured mRNA expression of duck FTH1 (duFTH1) in the liver and spleen after duck hepatitis virus 1 (DHV-1) infection using quantitative polymerase chain reaction (qPCR) and found that duFTH1 mRNA was down-regulated significantly in morbid ducklings (liver, P < 0.01; spleen, P < 0.05) compared with the control ducklings. We also found that duFTH1 expression was significantly higher in the spleen (P < 0.01) and liver (P < 0.05) of non-morbid ducklings than in morbid ducklings. Moreover, DNA methylation of the duFTH1 promoter was examined by bisulfite sequencing (BSP) and we found that the duFTH1 promoter was hypomethylated, the relative methylation was only 5.9% and 2.0% in the morbid ducklings and non-morbid ducklings, respectively. The promoter contained a -55 C/T mutation in 75% of non-morbid ducklings, and this polymorphism affected promoter activity. Further analysis suggested that this mutation altered the binding site of the transcription factor NRF1. Binding of NRF1 to the FTH1 promoter was confirmed by electrophoretic mobility shift assay (EMSA) analysis. Thus, our findings revealed the NRF1 was a negative regulator, and lossed of binding of NRF1 to duFTH1 promoter due to -55C/T mutation enhances duFTH1 expression in non-morbid ducks, which provided molecular insights into the effect of duFTH1 expression via promoter polymorphisms, but not DNA methylation, in response to DHV-1 challenge." @default.
- W2766561570 created "2017-11-10" @default.
- W2766561570 creator A5016719258 @default.
- W2766561570 creator A5034019216 @default.
- W2766561570 creator A5037109602 @default.
- W2766561570 creator A5050180203 @default.
- W2766561570 creator A5067315531 @default.
- W2766561570 creator A5069445164 @default.
- W2766561570 creator A5071773009 @default.
- W2766561570 creator A5081489339 @default.
- W2766561570 date "2018-02-01" @default.
- W2766561570 modified "2023-10-18" @default.
- W2766561570 title "FTH1 expression is affected by promoter polymorphism and not DNA methylation in response to DHV-1 challenge in duck" @default.
- W2766561570 cites W1496244512 @default.
- W2766561570 cites W1553586051 @default.
- W2766561570 cites W1977032723 @default.
- W2766561570 cites W1977820989 @default.
- W2766561570 cites W1986179924 @default.
- W2766561570 cites W1990465549 @default.
- W2766561570 cites W1990535093 @default.
- W2766561570 cites W1991547229 @default.
- W2766561570 cites W1997753515 @default.
- W2766561570 cites W2003058284 @default.
- W2766561570 cites W2006525603 @default.
- W2766561570 cites W2012393759 @default.
- W2766561570 cites W2026997251 @default.
- W2766561570 cites W2032758675 @default.
- W2766561570 cites W2048554272 @default.
- W2766561570 cites W2050838847 @default.
- W2766561570 cites W2051504691 @default.
- W2766561570 cites W2054661538 @default.
- W2766561570 cites W2060658816 @default.
- W2766561570 cites W2083607265 @default.
- W2766561570 cites W2090338629 @default.
- W2766561570 cites W2118362278 @default.
- W2766561570 cites W2123082386 @default.
- W2766561570 cites W2123742288 @default.
- W2766561570 cites W2143556012 @default.
- W2766561570 cites W2157888892 @default.
- W2766561570 cites W2164788576 @default.
- W2766561570 cites W2318444336 @default.
- W2766561570 cites W2471718007 @default.
- W2766561570 cites W2533523574 @default.
- W2766561570 cites W2590432101 @default.
- W2766561570 cites W2912111745 @default.
- W2766561570 doi "https://doi.org/10.1016/j.dci.2017.10.006" @default.
- W2766561570 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29051032" @default.
- W2766561570 hasPublicationYear "2018" @default.
- W2766561570 type Work @default.
- W2766561570 sameAs 2766561570 @default.
- W2766561570 citedByCount "3" @default.
- W2766561570 countsByYear W27665615702019 @default.
- W2766561570 countsByYear W27665615702020 @default.
- W2766561570 crossrefType "journal-article" @default.
- W2766561570 hasAuthorship W2766561570A5016719258 @default.
- W2766561570 hasAuthorship W2766561570A5034019216 @default.
- W2766561570 hasAuthorship W2766561570A5037109602 @default.
- W2766561570 hasAuthorship W2766561570A5050180203 @default.
- W2766561570 hasAuthorship W2766561570A5067315531 @default.
- W2766561570 hasAuthorship W2766561570A5069445164 @default.
- W2766561570 hasAuthorship W2766561570A5071773009 @default.
- W2766561570 hasAuthorship W2766561570A5081489339 @default.
- W2766561570 hasConcept C101762097 @default.
- W2766561570 hasConcept C104317684 @default.
- W2766561570 hasConcept C150194340 @default.
- W2766561570 hasConcept C153911025 @default.
- W2766561570 hasConcept C183978625 @default.
- W2766561570 hasConcept C190727270 @default.
- W2766561570 hasConcept C33288867 @default.
- W2766561570 hasConcept C54355233 @default.
- W2766561570 hasConcept C86803240 @default.
- W2766561570 hasConceptScore W2766561570C101762097 @default.
- W2766561570 hasConceptScore W2766561570C104317684 @default.
- W2766561570 hasConceptScore W2766561570C150194340 @default.
- W2766561570 hasConceptScore W2766561570C153911025 @default.
- W2766561570 hasConceptScore W2766561570C183978625 @default.
- W2766561570 hasConceptScore W2766561570C190727270 @default.
- W2766561570 hasConceptScore W2766561570C33288867 @default.
- W2766561570 hasConceptScore W2766561570C54355233 @default.
- W2766561570 hasConceptScore W2766561570C86803240 @default.
- W2766561570 hasFunder F4320322769 @default.
- W2766561570 hasLocation W27665615701 @default.
- W2766561570 hasLocation W27665615702 @default.
- W2766561570 hasOpenAccess W2766561570 @default.
- W2766561570 hasPrimaryLocation W27665615701 @default.
- W2766561570 hasRelatedWork W1992713540 @default.
- W2766561570 hasRelatedWork W1999023151 @default.
- W2766561570 hasRelatedWork W2024663868 @default.
- W2766561570 hasRelatedWork W2111485719 @default.
- W2766561570 hasRelatedWork W2144473770 @default.
- W2766561570 hasRelatedWork W2415020212 @default.
- W2766561570 hasRelatedWork W2992224717 @default.
- W2766561570 hasRelatedWork W3027569065 @default.
- W2766561570 hasRelatedWork W4210961801 @default.
- W2766561570 hasRelatedWork W2139377262 @default.
- W2766561570 isParatext "false" @default.
- W2766561570 isRetracted "false" @default.
- W2766561570 magId "2766561570" @default.
- W2766561570 workType "article" @default.