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- W2766659278 abstract "Endocytosis, the formation of membrane vesicles from the plasma membrane, is an essential feature of eukaryotic cell biology. Intense research effort has been dedicated to developing methods that can detect endocytosis events with the highest resolution. We have classified these methods into four families. They exploit the physical properties of endocytosis, namely: 1. Distinguishing extracellular from internalised cargo in fixed samples, 2. Monitoring endosomal acidification, 3. Measuring the turnover of endocytic zones and 4. Detecting vesicle scission. The last three families, all based on fluorescence imaging, are used to study endocytosis in living cells. We discuss the advantages and limitations of these methods and conclude on the future developments required to tackle the upcoming challenges in this fundamental field of cell biology." @default.
- W2766659278 created "2017-11-10" @default.
- W2766659278 creator A5029860180 @default.
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- W2766659278 date "2017-12-01" @default.
- W2766659278 modified "2023-10-18" @default.
- W2766659278 title "Imaging in focus: Imaging the dynamics of endocytosis" @default.
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- W2766659278 doi "https://doi.org/10.1016/j.biocel.2017.10.010" @default.
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