FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2766809224> ?p ?o ?g. }

• W2766809224 endingPage "e1006953" @default.
• W2766809224 startingPage "e1006953" @default.
• W2766809224 abstract "Divergence of upstream regulatory pathways of the transcription factor Doublesex (Dsx) serves as a basis for evolution of sex-determining mechanisms in animals. However, little is known about the regulation of Dsx in environmental sex determination. In the crustacean Daphnia magna, environmental sex determination is implemented by male-specific expression of the Dsx ortholog, Dsx1. Transcriptional regulation of Dsx1 comprises at least three phases during embryogenesis: non-sex-specific initiation, male-specific up-regulation, and its maintenance. Herein, we demonstrate that the male-specific up-regulation is controlled by the bZIP transcription factor, Vrille (Vri), an ortholog of the circadian clock genes—Drosophila Vri and mammalian E4BP4/NFIL3. Sequence analysis of the Dsx1 promoter/enhancer revealed a conserved element among two Daphnia species (D. magna and D. pulex), which contains a potential enhancer harboring a consensus Vri binding site overlapped with a consensus Dsx binding site. Besides non-sex-specific expression of Vri in late embryos, we found male-specific expression in early gastrula before the Dsx1 up-regulation phase begins. Knockdown of Vri in male embryos showed reduction of Dsx1 expression. In addition, transient overexpression of Vri in early female embryos up-regulated the expression of Dsx1 and induced male-specific trait. Targeted mutagenesis using CRISPR/Cas9 disrupted the enhancer on genome in males, which led to the reduction of Dsx1 expression. These results indicate that Vri was co-opted as a transcriptional activator of Dsx1 in environmental sex determination of D. magna. The data suggests the remarkably plastic nature of gene regulatory network in sex determination." @default.
• W2766809224 created "2017-11-10" @default.
• W2766809224 creator A5005879454 @default.
• W2766809224 creator A5011463853 @default.
• W2766809224 creator A5041139506 @default.
• W2766809224 creator A5055034827 @default.
• W2766809224 creator A5074624931 @default.
• W2766809224 date "2017-11-02" @default.
• W2766809224 modified "2023-09-27" @default.
• W2766809224 title "Co-option of the bZIP transcription factor Vrille as the activator of Doublesex1 in environmental sex determination of the crustacean Daphnia magna" @default.
• W2766809224 cites W1577577364 @default.
• W2766809224 cites W1579000626 @default.
• W2766809224 cites W1691484617 @default.
• W2766809224 cites W1878468064 @default.
• W2766809224 cites W1967947254 @default.
• W2766809224 cites W1971442608 @default.
• W2766809224 cites W1972705715 @default.
• W2766809224 cites W1981920912 @default.
• W2766809224 cites W1990335021 @default.
• W2766809224 cites W1991215309 @default.
• W2766809224 cites W1993246714 @default.
• W2766809224 cites W1996777369 @default.
• W2766809224 cites W2000479887 @default.
• W2766809224 cites W2007969716 @default.
• W2766809224 cites W2012262982 @default.
• W2766809224 cites W2017665051 @default.
• W2766809224 cites W2025885587 @default.
• W2766809224 cites W2033500368 @default.
• W2766809224 cites W2049147637 @default.
• W2766809224 cites W2050223231 @default.
• W2766809224 cites W2053742351 @default.
• W2766809224 cites W2059233607 @default.
• W2766809224 cites W2060250559 @default.
• W2766809224 cites W2068771737 @default.
• W2766809224 cites W2070704344 @default.
• W2766809224 cites W2075458954 @default.
• W2766809224 cites W2084273299 @default.
• W2766809224 cites W2085139131 @default.
• W2766809224 cites W2085421027 @default.
• W2766809224 cites W2086721579 @default.
• W2766809224 cites W2090632804 @default.
• W2766809224 cites W2095301042 @default.
• W2766809224 cites W2096819533 @default.
• W2766809224 cites W2102190537 @default.
• W2766809224 cites W2104596514 @default.
• W2766809224 cites W2107405297 @default.
• W2766809224 cites W2113529963 @default.
• W2766809224 cites W2118498394 @default.
• W2766809224 cites W2126122040 @default.
• W2766809224 cites W2132928157 @default.
• W2766809224 cites W2140166139 @default.
• W2766809224 cites W2154405295 @default.
• W2766809224 cites W2154975687 @default.
• W2766809224 cites W2155637628 @default.
• W2766809224 cites W2156371935 @default.
• W2766809224 cites W2164224520 @default.
• W2766809224 cites W2164239098 @default.
• W2766809224 cites W2164695230 @default.
• W2766809224 cites W2280219571 @default.
• W2766809224 cites W2321114722 @default.
• W2766809224 cites W2345537204 @default.
• W2766809224 cites W2442832596 @default.
• W2766809224 cites W2552239481 @default.
• W2766809224 cites W2572193671 @default.
• W2766809224 cites W2596255045 @default.
• W2766809224 cites W2761787345 @default.
• W2766809224 cites W3025449462 @default.
• W2766809224 doi "https://doi.org/10.1371/journal.pgen.1006953" @default.
• W2766809224 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5667737" @default.
• W2766809224 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29095827" @default.
• W2766809224 hasPublicationYear "2017" @default.
• W2766809224 type Work @default.
• W2766809224 sameAs 2766809224 @default.
• W2766809224 citedByCount "23" @default.
• W2766809224 countsByYear W27668092242017 @default.
• W2766809224 countsByYear W27668092242018 @default.
• W2766809224 countsByYear W27668092242019 @default.
• W2766809224 countsByYear W27668092242020 @default.
• W2766809224 countsByYear W27668092242021 @default.
• W2766809224 countsByYear W27668092242022 @default.
• W2766809224 countsByYear W27668092242023 @default.
• W2766809224 crossrefType "journal-article" @default.
• W2766809224 hasAuthorship W2766809224A5005879454 @default.
• W2766809224 hasAuthorship W2766809224A5011463853 @default.
• W2766809224 hasAuthorship W2766809224A5041139506 @default.
• W2766809224 hasAuthorship W2766809224A5055034827 @default.
• W2766809224 hasAuthorship W2766809224A5074624931 @default.
• W2766809224 hasBestOaLocation W27668092241 @default.
• W2766809224 hasConcept C101762097 @default.
• W2766809224 hasConcept C104317684 @default.
• W2766809224 hasConcept C111936080 @default.
• W2766809224 hasConcept C126322002 @default.
• W2766809224 hasConcept C150194340 @default.
• W2766809224 hasConcept C173396325 @default.
• W2766809224 hasConcept C18903297 @default.
• W2766809224 hasConcept C2776514529 @default.
• W2766809224 hasConcept C2776878037 @default.
• W2766809224 hasConcept C2779765374 @default.

• next