FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2766819331> ?p ?o ?g. }

• W2766819331 endingPage "3862" @default.
• W2766819331 startingPage "3850" @default.
• W2766819331 abstract "The marine osmolyte dimethylsulfoniopropionate (DMSP) is one of Earth's most abundant organosulfur molecules. Bacterial DMSP lyases cleave DMSP, producing acrylate and dimethyl sulfide (DMS), a climate-active gas with roles in global sulfur cycling and atmospheric chemistry. DddY is the only known periplasmic DMSP lyase and is present in β-, γ-, δ- and ε-proteobacteria. Unlike other known DMSP lyases, DddY has not been classified into a protein superfamily, and its structure and catalytic mechanism are unknown. Here, we determined the crystal structure of DddY from the γ-proteobacterium Acinetobacter bereziniae originally isolated from human clinical specimens. This structure revealed that DddY contains a cap domain and a catalytic domain with a Zn2 + bound at its active site. We also observed that the DddY catalytic domain adopts a typical β-barrel fold and contains two conserved cupin motifs. Therefore, we concluded that DddY should belong to the cupin superfamily. Using structural and mutational analyses, we identified key residues involved in Zn2 + coordination, DMSP binding and the catalysis of DMSP cleavage, enabling elucidation of the catalytic mechanism, in which the residue Tyr271 of DddY acts as a general base to attack DMSP. Moreover, sequence analysis suggested that this proposed mechanism is common to DddY proteins from β-, γ-, δ- and ε-proteobacteria. The DddY structure and proposed catalytic mechanism provide a better understanding of how DMSP is catabolized to generate the important climate-active gas DMS." @default.
• W2766819331 created "2017-11-10" @default.
• W2766819331 creator A5018449221 @default.
• W2766819331 creator A5027099198 @default.
• W2766819331 creator A5049078993 @default.
• W2766819331 creator A5056862578 @default.
• W2766819331 creator A5059257819 @default.
• W2766819331 creator A5066796969 @default.
• W2766819331 creator A5073156841 @default.
• W2766819331 creator A5078903262 @default.
• W2766819331 creator A5087514389 @default.
• W2766819331 creator A5088092758 @default.
• W2766819331 date "2017-12-01" @default.
• W2766819331 modified "2023-10-15" @default.
• W2766819331 title "Mechanistic Insights into Dimethylsulfoniopropionate Lyase DddY, a New Member of the Cupin Superfamily" @default.
• W2766819331 cites W1527006796 @default.
• W2766819331 cites W1549222782 @default.
• W2766819331 cites W1922529518 @default.
• W2766819331 cites W1939931704 @default.
• W2766819331 cites W1969524849 @default.
• W2766819331 cites W1982254235 @default.
• W2766819331 cites W1986678287 @default.
• W2766819331 cites W1990469238 @default.
• W2766819331 cites W1991422857 @default.
• W2766819331 cites W1993795153 @default.
• W2766819331 cites W1999409076 @default.
• W2766819331 cites W2003497790 @default.
• W2766819331 cites W2015434885 @default.
• W2766819331 cites W2020108475 @default.
• W2766819331 cites W2030517878 @default.
• W2766819331 cites W2037253128 @default.
• W2766819331 cites W2038081998 @default.
• W2766819331 cites W2040204238 @default.
• W2766819331 cites W2055148441 @default.
• W2766819331 cites W2056427207 @default.
• W2766819331 cites W2071626387 @default.
• W2766819331 cites W2078336779 @default.
• W2766819331 cites W2095724872 @default.
• W2766819331 cites W2103503400 @default.
• W2766819331 cites W2104822255 @default.
• W2766819331 cites W2108921801 @default.
• W2766819331 cites W2115078227 @default.
• W2766819331 cites W2122698931 @default.
• W2766819331 cites W2124026197 @default.
• W2766819331 cites W2129499489 @default.
• W2766819331 cites W2130207501 @default.
• W2766819331 cites W2134501609 @default.
• W2766819331 cites W2136985449 @default.
• W2766819331 cites W2138260806 @default.
• W2766819331 cites W2152207030 @default.
• W2766819331 cites W2153745071 @default.
• W2766819331 cites W2168974130 @default.
• W2766819331 cites W2180229411 @default.
• W2766819331 cites W2268090057 @default.
• W2766819331 cites W2323429880 @default.
• W2766819331 cites W2404677456 @default.
• W2766819331 cites W2588658196 @default.
• W2766819331 cites W2616154525 @default.
• W2766819331 cites W67612493 @default.
• W2766819331 doi "https://doi.org/10.1016/j.jmb.2017.10.022" @default.
• W2766819331 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29106934" @default.
• W2766819331 hasPublicationYear "2017" @default.
• W2766819331 type Work @default.
• W2766819331 sameAs 2766819331 @default.
• W2766819331 citedByCount "21" @default.
• W2766819331 countsByYear W27668193312018 @default.
• W2766819331 countsByYear W27668193312019 @default.
• W2766819331 countsByYear W27668193312020 @default.
• W2766819331 countsByYear W27668193312021 @default.
• W2766819331 countsByYear W27668193312022 @default.
• W2766819331 countsByYear W27668193312023 @default.
• W2766819331 crossrefType "journal-article" @default.
• W2766819331 hasAuthorship W2766819331A5018449221 @default.
• W2766819331 hasAuthorship W2766819331A5027099198 @default.
• W2766819331 hasAuthorship W2766819331A5049078993 @default.
• W2766819331 hasAuthorship W2766819331A5056862578 @default.
• W2766819331 hasAuthorship W2766819331A5059257819 @default.
• W2766819331 hasAuthorship W2766819331A5066796969 @default.
• W2766819331 hasAuthorship W2766819331A5073156841 @default.
• W2766819331 hasAuthorship W2766819331A5078903262 @default.
• W2766819331 hasAuthorship W2766819331A5087514389 @default.
• W2766819331 hasAuthorship W2766819331A5088092758 @default.
• W2766819331 hasBestOaLocation W27668193311 @default.
• W2766819331 hasConcept C127160389 @default.
• W2766819331 hasConcept C142796444 @default.
• W2766819331 hasConcept C178790620 @default.
• W2766819331 hasConcept C181199279 @default.
• W2766819331 hasConcept C185592680 @default.
• W2766819331 hasConcept C2777269803 @default.
• W2766819331 hasConcept C2777593447 @default.
• W2766819331 hasConcept C2778413813 @default.
• W2766819331 hasConcept C2780892065 @default.
• W2766819331 hasConcept C518881349 @default.
• W2766819331 hasConcept C55493867 @default.
• W2766819331 hasConcept C71240020 @default.
• W2766819331 hasConceptScore W2766819331C127160389 @default.
• W2766819331 hasConceptScore W2766819331C142796444 @default.
• W2766819331 hasConceptScore W2766819331C178790620 @default.

• next