Matches in SemOpenAlex for { <https://semopenalex.org/work/W2767143710> ?p ?o ?g. }
- W2767143710 endingPage "965" @default.
- W2767143710 startingPage "955" @default.
- W2767143710 abstract "Actinomycete secondary metabolites are a renowned source of antibacterial chemical scaffolds. Herein, we present a target-specific approach that increases the detection of antimetabolites from natural sources by screening actinomycete-derived extracts against nutrient transporter deletion strains. On the basis of the growth rescue patterns of a collection of 22 Escherichia coli (E. coli) auxotrophic deletion strains representative of the major nutrient biosynthetic pathways, we demonstrate that antimetabolite detection from actinomycete-derived extracts prepared using traditional extraction platforms is masked by nutrient supplementation. In particular, we find poor sensitivity for the detection of antimetabolites targeting vitamin biosynthesis. To circumvent this and as a proof of principle, we exploit the differential activity of actinomycete extracts against E. coli ΔyigM, a biotin transporter deletion strain versus wildtype E. coli. We achieve more than a 100-fold increase in antimetabolite sensitivity using this method and demonstrate a successful bioassay-guided purification of the known biotin antimetabolite, amiclenomycin. Our findings provide a unique solution to uncover the full potential of naturally derived antibiotics." @default.
- W2767143710 created "2017-11-10" @default.
- W2767143710 creator A5009717743 @default.
- W2767143710 creator A5011130173 @default.
- W2767143710 creator A5012876941 @default.
- W2767143710 creator A5042757211 @default.
- W2767143710 creator A5062591234 @default.
- W2767143710 creator A5076663151 @default.
- W2767143710 creator A5080982794 @default.
- W2767143710 creator A5087255559 @default.
- W2767143710 creator A5090927714 @default.
- W2767143710 date "2017-11-07" @default.
- W2767143710 modified "2023-09-27" @default.
- W2767143710 title "Exploiting the Sensitivity of Nutrient Transporter Deletion Strains in Discovery of Natural Product Antimetabolites" @default.
- W2767143710 cites W1561843798 @default.
- W2767143710 cites W1797716508 @default.
- W2767143710 cites W1858789088 @default.
- W2767143710 cites W1963449091 @default.
- W2767143710 cites W1968146426 @default.
- W2767143710 cites W1970975622 @default.
- W2767143710 cites W1978970152 @default.
- W2767143710 cites W1988364643 @default.
- W2767143710 cites W1999068704 @default.
- W2767143710 cites W2007857508 @default.
- W2767143710 cites W2012960603 @default.
- W2767143710 cites W2019879105 @default.
- W2767143710 cites W2021093384 @default.
- W2767143710 cites W2023157051 @default.
- W2767143710 cites W2024595910 @default.
- W2767143710 cites W2025155908 @default.
- W2767143710 cites W2035133853 @default.
- W2767143710 cites W2036561818 @default.
- W2767143710 cites W2037655495 @default.
- W2767143710 cites W2040285328 @default.
- W2767143710 cites W2046637912 @default.
- W2767143710 cites W2051153330 @default.
- W2767143710 cites W2057717820 @default.
- W2767143710 cites W2059520394 @default.
- W2767143710 cites W2065781312 @default.
- W2767143710 cites W2070104235 @default.
- W2767143710 cites W2078355424 @default.
- W2767143710 cites W2079005811 @default.
- W2767143710 cites W2081183202 @default.
- W2767143710 cites W2097694433 @default.
- W2767143710 cites W2097951029 @default.
- W2767143710 cites W2102130732 @default.
- W2767143710 cites W2106084243 @default.
- W2767143710 cites W2106233702 @default.
- W2767143710 cites W2108078368 @default.
- W2767143710 cites W2108153887 @default.
- W2767143710 cites W2111967267 @default.
- W2767143710 cites W2115089227 @default.
- W2767143710 cites W2124320287 @default.
- W2767143710 cites W2125088742 @default.
- W2767143710 cites W2126693364 @default.
- W2767143710 cites W2128951021 @default.
- W2767143710 cites W2129273969 @default.
- W2767143710 cites W2131494781 @default.
- W2767143710 cites W2133870853 @default.
- W2767143710 cites W2140354550 @default.
- W2767143710 cites W2150316935 @default.
- W2767143710 cites W2153584471 @default.
- W2767143710 cites W2158696212 @default.
- W2767143710 cites W2169163713 @default.
- W2767143710 cites W2331053199 @default.
- W2767143710 cites W2332824477 @default.
- W2767143710 cites W2552981562 @default.
- W2767143710 cites W41459149 @default.
- W2767143710 cites W589727570 @default.
- W2767143710 doi "https://doi.org/10.1021/acsinfecdis.7b00149" @default.
- W2767143710 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29069544" @default.
- W2767143710 hasPublicationYear "2017" @default.
- W2767143710 type Work @default.
- W2767143710 sameAs 2767143710 @default.
- W2767143710 citedByCount "11" @default.
- W2767143710 countsByYear W27671437102017 @default.
- W2767143710 countsByYear W27671437102019 @default.
- W2767143710 countsByYear W27671437102021 @default.
- W2767143710 countsByYear W27671437102022 @default.
- W2767143710 countsByYear W27671437102023 @default.
- W2767143710 crossrefType "journal-article" @default.
- W2767143710 hasAuthorship W2767143710A5009717743 @default.
- W2767143710 hasAuthorship W2767143710A5011130173 @default.
- W2767143710 hasAuthorship W2767143710A5012876941 @default.
- W2767143710 hasAuthorship W2767143710A5042757211 @default.
- W2767143710 hasAuthorship W2767143710A5062591234 @default.
- W2767143710 hasAuthorship W2767143710A5076663151 @default.
- W2767143710 hasAuthorship W2767143710A5080982794 @default.
- W2767143710 hasAuthorship W2767143710A5087255559 @default.
- W2767143710 hasAuthorship W2767143710A5090927714 @default.
- W2767143710 hasBestOaLocation W27671437101 @default.
- W2767143710 hasConcept C104317684 @default.
- W2767143710 hasConcept C104488531 @default.
- W2767143710 hasConcept C20950039 @default.
- W2767143710 hasConcept C2776694085 @default.
- W2767143710 hasConcept C2778204606 @default.
- W2767143710 hasConcept C2778397455 @default.
- W2767143710 hasConcept C2779396153 @default.