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- W2767251572 abstract "Ewa K Krasnowska, Enrico Gratton, and Tiziana Parasassi. Cholesterol influences PRODAN partitioning between phospholipid phases and water. 43rd Annual Meeting of the Biophysical Society, Baltimore, Maryland, 1999. Biophys J. 1999; 76(1 Pt 2). Abstract The effect of cholesterol on the packing and polarity of bilayers has been studied by the fluorescent probe 6-propionyl-2-dimethylaminonaphthalene (PRODAN), a probe located at the membrane surface. In the gel phospholipid phase and in the absence of cholesterol, PRODAN's phospholipid:water partition coefficient has been spectroscopically determined using the fluorescence intensities at three emission wavelengths and was about 14 × 104. In the liquid- crystalline phase of the bilayer this value was about 500 × 104 (Krasnowska et al., Biophys. J., 1998, 74:1984). PRODAN fluorescence emission from the aqueous environment was monitored by the appearance of a red emission band centered at 520 nm. When cholesterol was added to phospholipid gel phase bilayer, at concentrations >3mol%, the red emission band centered at 520 nm disappeared and the PRODAN partition coefficient was similar to that determined for liquid-crystalline phase bilayers. We attributed this effect to cholesterol- induced looser interactions between adjacent phospholipid polar head groups that allowed a better penetration of PRODAN molecules into the membrane. In gel phase bilayers, the PRODAN partition coefficient was a function of cholesterol concentration. By increasing cholesterol concentration the probe partitioning in the bilayer showed a linear and relevant increase, up to 10 mol% cholesterol. At higher cholesterol concentrations, the probe partition coefficient was about constant up to 30 mol%. PRODAN's spectral features were also used to monitor small variations occurring in the packing of the bilayer surface for fine variations of cholesterol concentration, from 20 to 30 mol%, with 1 mol% steps. Due to PRODAN's higher sensitivity ... [truncated at 250 words]" @default.
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- W2767251572 date "1999-01-01" @default.
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- W2767251572 title "Cholesterol influences PRODAN partitioning between phospholipid phases and water." @default.
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