FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2767445377> ?p ?o ?g. }

• W2767445377 endingPage "225" @default.
• W2767445377 startingPage "218" @default.
• W2767445377 abstract "Optimised purification steps for concentrating trace target native antigens are needed. Combining the p-aminobenzamidine ligand with protease inactivation enables partial purification of mite non-protease allergens lacking proteases.We sought to analyse in detail proteins obtained using this method from eight species of synanthropic acaridid mites and tested IgE reactivity using pooled human sera.Proteins affinity bound to p-aminobenzamidine as a ligand were identified by MALDI TOF/TOF. After electroblotting, the proteins were visualised using the fluorescent SYPRO-Ruby protein blot stain, and IgE reactivity was further analysed using pooled human sera collected from patients allergic to house dust mites.MS/MS identification confirmed previous results that no proteases were purified. Protein patterns corresponding to the allergens Der f 7, Der f 30 and actins indicated that these proteins are purified using p-aminobenzamidine and are present across a wide spectrum of acaridid mites. When using Dermatophagoides farinae, apolipophorins (Der f 14), chitinase-like Der f 15 and 18, 70-kDa heat shock protein, and a Der f Alt a10 allergen homolog (gi|37958173) were also detected. The target antigens tropomyosins and paramyosins showed similar IgE binding among the mite species tested. IgE reactivity with miscellaneous D. farinae antigen was also observed.Partial purification of mite non-protease antigens using a strategy combining p-aminobenzamidine with protease inactivation was verified by 1D-E and 2D-E analyses. IgE binding to p-aminobenzamidine-purified native non-protease mite antigens was tested using pooled sera. This preliminary study allows for further work on individual serum samples, allowing confirmation of immunoreactivity." @default.
• W2767445377 created "2017-11-17" @default.
• W2767445377 creator A5028121069 @default.
• W2767445377 creator A5060388384 @default.
• W2767445377 date "2018-05-01" @default.
• W2767445377 modified "2023-09-24" @default.
• W2767445377 title "Non-protease native allergens partially purified from bodies of eight domestic mites using p-aminobenzamidine ligand" @default.
• W2767445377 cites W1481873398 @default.
• W2767445377 cites W1513838388 @default.
• W2767445377 cites W1595932129 @default.
• W2767445377 cites W1964229883 @default.
• W2767445377 cites W1966108601 @default.
• W2767445377 cites W1970824456 @default.
• W2767445377 cites W1982279940 @default.
• W2767445377 cites W1991645244 @default.
• W2767445377 cites W1999765352 @default.
• W2767445377 cites W2002612707 @default.
• W2767445377 cites W2005551859 @default.
• W2767445377 cites W2010658395 @default.
• W2767445377 cites W2028824489 @default.
• W2767445377 cites W2035847981 @default.
• W2767445377 cites W2040259894 @default.
• W2767445377 cites W2050794863 @default.
• W2767445377 cites W2052290049 @default.
• W2767445377 cites W2057637457 @default.
• W2767445377 cites W2060315709 @default.
• W2767445377 cites W2060643604 @default.
• W2767445377 cites W2063553907 @default.
• W2767445377 cites W2066612331 @default.
• W2767445377 cites W2080948057 @default.
• W2767445377 cites W2088185101 @default.
• W2767445377 cites W2088618841 @default.
• W2767445377 cites W2091674587 @default.
• W2767445377 cites W2103249295 @default.
• W2767445377 cites W2114757629 @default.
• W2767445377 cites W2128845826 @default.
• W2767445377 cites W2129108840 @default.
• W2767445377 cites W2158714788 @default.
• W2767445377 cites W2164390562 @default.
• W2767445377 cites W2169959116 @default.
• W2767445377 cites W2412548038 @default.
• W2767445377 cites W2606426472 @default.
• W2767445377 doi "https://doi.org/10.1016/j.aller.2017.07.004" @default.
• W2767445377 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29128091" @default.
• W2767445377 hasPublicationYear "2018" @default.
• W2767445377 type Work @default.
• W2767445377 sameAs 2767445377 @default.
• W2767445377 citedByCount "2" @default.
• W2767445377 countsByYear W27674453772020 @default.
• W2767445377 countsByYear W27674453772021 @default.
• W2767445377 crossrefType "journal-article" @default.
• W2767445377 hasAuthorship W2767445377A5028121069 @default.
• W2767445377 hasAuthorship W2767445377A5060388384 @default.
• W2767445377 hasConcept C104317684 @default.
• W2767445377 hasConcept C141105273 @default.
• W2767445377 hasConcept C147483822 @default.
• W2767445377 hasConcept C153911025 @default.
• W2767445377 hasConcept C159654299 @default.
• W2767445377 hasConcept C179247698 @default.
• W2767445377 hasConcept C181199279 @default.
• W2767445377 hasConcept C182220744 @default.
• W2767445377 hasConcept C185592680 @default.
• W2767445377 hasConcept C203014093 @default.
• W2767445377 hasConcept C207480886 @default.
• W2767445377 hasConcept C2776415932 @default.
• W2767445377 hasConcept C2776714187 @default.
• W2767445377 hasConcept C2778166374 @default.
• W2767445377 hasConcept C2779711295 @default.
• W2767445377 hasConcept C2780510475 @default.
• W2767445377 hasConcept C55493867 @default.
• W2767445377 hasConcept C59822182 @default.
• W2767445377 hasConcept C86803240 @default.
• W2767445377 hasConceptScore W2767445377C104317684 @default.
• W2767445377 hasConceptScore W2767445377C141105273 @default.
• W2767445377 hasConceptScore W2767445377C147483822 @default.
• W2767445377 hasConceptScore W2767445377C153911025 @default.
• W2767445377 hasConceptScore W2767445377C159654299 @default.
• W2767445377 hasConceptScore W2767445377C179247698 @default.
• W2767445377 hasConceptScore W2767445377C181199279 @default.
• W2767445377 hasConceptScore W2767445377C182220744 @default.
• W2767445377 hasConceptScore W2767445377C185592680 @default.
• W2767445377 hasConceptScore W2767445377C203014093 @default.
• W2767445377 hasConceptScore W2767445377C207480886 @default.
• W2767445377 hasConceptScore W2767445377C2776415932 @default.
• W2767445377 hasConceptScore W2767445377C2776714187 @default.
• W2767445377 hasConceptScore W2767445377C2778166374 @default.
• W2767445377 hasConceptScore W2767445377C2779711295 @default.
• W2767445377 hasConceptScore W2767445377C2780510475 @default.
• W2767445377 hasConceptScore W2767445377C55493867 @default.
• W2767445377 hasConceptScore W2767445377C59822182 @default.
• W2767445377 hasConceptScore W2767445377C86803240 @default.
• W2767445377 hasIssue "3" @default.
• W2767445377 hasLocation W27674453771 @default.
• W2767445377 hasLocation W27674453772 @default.
• W2767445377 hasOpenAccess W2767445377 @default.
• W2767445377 hasPrimaryLocation W27674453771 @default.
• W2767445377 hasRelatedWork W1553155140 @default.
• W2767445377 hasRelatedWork W1978801742 @default.

• next