FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2767477047> ?p ?o ?g. }

• W2767477047 endingPage "4188" @default.
• W2767477047 startingPage "4180" @default.
• W2767477047 abstract "We report the development of a novel flow cytometry-based Ig capture assay (ICA) for the identification and sorting of individual Ab-secreting cells based on their Ag reactivity. The ICA represents a fast and versatile tool for single-cell sorting of peripheral plasmablasts, streamlining subsequent Ab analysis, and cloning. We demonstrate the utility of the assay by isolating Ag-reactive plasmablasts from cryopreserved PBMC obtained from volunteers vaccinated with a recombinant HIV envelope protein. To show the specificity of the ICA, we produced Ag-specific Abs from these cells and subsequently verified their Ag reactivity via ELISA. Furthermore, we used the ICA to track Ag-specific plasmablast responses in HIV-vaccine recipients over a period of 42 d and performed a head-to-head comparison with a conventional B cell ELISpot. Results were highly comparable, highlighting that this assay is a viable alternative for monitoring Ag-specific plasmablast responses at early time points after infection or vaccination. The ICA provides important added benefits in that phenotypic information can be obtained from the identified Ag-specific cells that can then be captured for downstream applications such as B cell sequencing and/or Ab cloning. We envisage the ICA as being a useful tool in Ab repertoire analysis for future clinical trials." @default.
• W2767477047 created "2017-11-17" @default.
• W2767477047 creator A5038096464 @default.
• W2767477047 creator A5039511985 @default.
• W2767477047 creator A5040670585 @default.
• W2767477047 creator A5044967548 @default.
• W2767477047 creator A5069492259 @default.
• W2767477047 creator A5086956796 @default.
• W2767477047 creator A5089276634 @default.
• W2767477047 date "2017-12-15" @default.
• W2767477047 modified "2023-10-18" @default.
• W2767477047 title "Isolation and Characterization of Antigen-Specific Plasmablasts Using a Novel Flow Cytometry–Based Ig Capture Assay" @default.
• W2767477047 cites W1534432347 @default.
• W2767477047 cites W1925989912 @default.
• W2767477047 cites W1974428785 @default.
• W2767477047 cites W1980394026 @default.
• W2767477047 cites W1996626942 @default.
• W2767477047 cites W2002206719 @default.
• W2767477047 cites W2011136801 @default.
• W2767477047 cites W2015554529 @default.
• W2767477047 cites W2016383718 @default.
• W2767477047 cites W2034736633 @default.
• W2767477047 cites W2041078707 @default.
• W2767477047 cites W2048421209 @default.
• W2767477047 cites W2054109196 @default.
• W2767477047 cites W2058078577 @default.
• W2767477047 cites W2065889873 @default.
• W2767477047 cites W2073379765 @default.
• W2767477047 cites W2077644183 @default.
• W2767477047 cites W2096510930 @default.
• W2767477047 cites W2105858955 @default.
• W2767477047 cites W2107649987 @default.
• W2767477047 cites W2107723887 @default.
• W2767477047 cites W2119779320 @default.
• W2767477047 cites W2123708888 @default.
• W2767477047 cites W2129509656 @default.
• W2767477047 cites W2131953925 @default.
• W2767477047 cites W2280935809 @default.
• W2767477047 cites W2462706683 @default.
• W2767477047 cites W2520582555 @default.
• W2767477047 cites W2580241639 @default.
• W2767477047 cites W2582689618 @default.
• W2767477047 cites W2620312500 @default.
• W2767477047 doi "https://doi.org/10.4049/jimmunol.1701253" @default.
• W2767477047 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5720343" @default.
• W2767477047 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29118244" @default.
• W2767477047 hasPublicationYear "2017" @default.
• W2767477047 type Work @default.
• W2767477047 sameAs 2767477047 @default.
• W2767477047 citedByCount "25" @default.
• W2767477047 countsByYear W27674770472018 @default.
• W2767477047 countsByYear W27674770472019 @default.
• W2767477047 countsByYear W27674770472020 @default.
• W2767477047 countsByYear W27674770472021 @default.
• W2767477047 countsByYear W27674770472022 @default.
• W2767477047 countsByYear W27674770472023 @default.
• W2767477047 crossrefType "journal-article" @default.
• W2767477047 hasAuthorship W2767477047A5038096464 @default.
• W2767477047 hasAuthorship W2767477047A5039511985 @default.
• W2767477047 hasAuthorship W2767477047A5040670585 @default.
• W2767477047 hasAuthorship W2767477047A5044967548 @default.
• W2767477047 hasAuthorship W2767477047A5069492259 @default.
• W2767477047 hasAuthorship W2767477047A5086956796 @default.
• W2767477047 hasAuthorship W2767477047A5089276634 @default.
• W2767477047 hasBestOaLocation W27674770471 @default.
• W2767477047 hasConcept C104317684 @default.
• W2767477047 hasConcept C147483822 @default.
• W2767477047 hasConcept C153911025 @default.
• W2767477047 hasConcept C159654299 @default.
• W2767477047 hasConcept C203014093 @default.
• W2767477047 hasConcept C2776090121 @default.
• W2767477047 hasConcept C2778630268 @default.
• W2767477047 hasConcept C2779053233 @default.
• W2767477047 hasConcept C2780339063 @default.
• W2767477047 hasConcept C40767141 @default.
• W2767477047 hasConcept C54355233 @default.
• W2767477047 hasConcept C553184892 @default.
• W2767477047 hasConcept C70721500 @default.
• W2767477047 hasConcept C86803240 @default.
• W2767477047 hasConcept C8891405 @default.
• W2767477047 hasConceptScore W2767477047C104317684 @default.
• W2767477047 hasConceptScore W2767477047C147483822 @default.
• W2767477047 hasConceptScore W2767477047C153911025 @default.
• W2767477047 hasConceptScore W2767477047C159654299 @default.
• W2767477047 hasConceptScore W2767477047C203014093 @default.
• W2767477047 hasConceptScore W2767477047C2776090121 @default.
• W2767477047 hasConceptScore W2767477047C2778630268 @default.
• W2767477047 hasConceptScore W2767477047C2779053233 @default.
• W2767477047 hasConceptScore W2767477047C2780339063 @default.
• W2767477047 hasConceptScore W2767477047C40767141 @default.
• W2767477047 hasConceptScore W2767477047C54355233 @default.
• W2767477047 hasConceptScore W2767477047C553184892 @default.
• W2767477047 hasConceptScore W2767477047C70721500 @default.
• W2767477047 hasConceptScore W2767477047C86803240 @default.
• W2767477047 hasConceptScore W2767477047C8891405 @default.
• W2767477047 hasIssue "12" @default.
• W2767477047 hasLocation W27674770471 @default.
• W2767477047 hasLocation W27674770472 @default.

• next