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• W2767629076 abstract "Abstract The spinal dorsal horn (SDH) is comprised of distinct neuronal populations that process different somatosensory modalities. Somatostatin (SST)-expressing interneurons in the SDH have been implicated specifically in mediating mechanical pain. Identifying the transcriptomic profile of SST neurons could elucidate the unique genetic features of this population and enable selective analgesic targeting. To that end, we combined the Isolation of Nuclei Tagged in Specific Cell Types (INTACT) method and Fluorescence Activated Nuclei Sorting (FANS) to capture tagged SST nuclei in the SDH of adult male mice. Using RNA-sequencing (RNA-seq), we uncovered more than 13,000 genes. Differential gene expression analysis revealed more than 900 genes with at least 2-fold enrichment. In addition to many known dorsal horn genes, we identified and validated several novel transcripts from pharmacologically tractable functional classes: Carbonic Anhydrase 12 (Car12) , Phosphodiesterase 11A (Pde11a) , Protease-Activated Receptor 3 (F2rl2) and G-protein Coupled Receptor 26 (Gpr26). In situ hybridization of these novel genes revealed differential expression patterns in the SDH, demonstrating the presence of transcriptionally distinct subpopulations within the SST population. Pathway analysis revealed several enriched signaling pathways including cyclic AMP-mediated signaling, Nitric Oxide Synthase signaling, and voltage-gated calcium channels, highlighting the importance of these pathways to SST neuron function. Overall, our findings provide new insights into the gene repertoire of SST dorsal horn neurons and reveal several candidate targets for pharmacological modulation of this pain-mediating population. Significance Statement Somatostatin(SST)-expressing interneurons in the spinal dorsal horn (SDH) are required for the perception of mechanical pain. Identifying the distinctive genes expressed by SST neurons could facilitate the development of novel, circuit-targeting analgesics. Thus, we applied cell type-specific RNA-sequencing (RNA-seq) to provide the first transcriptional profile of SST neurons in the SDH. Bioinformatic analysis revealed hundreds of genes enriched in SST neurons, including several previously undescribed genes from druggable classes ( Car12, Pde11a, F2rl2 and Gpr26 ). Taken together, our study unveils a comprehensive transcriptional signature for SST neurons, highlights promising candidate genes for future analgesic development, and establishes a flexible method for transcriptional profiling of any spinal cord cell type." @default.
• W2767629076 created "2017-11-17" @default.
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• W2767629076 date "2017-11-08" @default.
• W2767629076 modified "2023-09-23" @default.
• W2767629076 title "Transcriptional Profiling of Somatostatin Interneurons in the Spinal Dorsal Horn" @default.
• W2767629076 cites W1552945128 @default.
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• W2767629076 cites W1969638732 @default.
• W2767629076 cites W1969751124 @default.
• W2767629076 cites W1970202520 @default.
• W2767629076 cites W1973094248 @default.
• W2767629076 cites W1977458672 @default.
• W2767629076 cites W1979283544 @default.
• W2767629076 cites W1980623757 @default.
• W2767629076 cites W1991738040 @default.
• W2767629076 cites W1994047748 @default.
• W2767629076 cites W2001279193 @default.
• W2767629076 cites W2002630238 @default.
• W2767629076 cites W2008175324 @default.
• W2767629076 cites W2015167895 @default.
• W2767629076 cites W2015471061 @default.
• W2767629076 cites W2016097940 @default.
• W2767629076 cites W2017384269 @default.
• W2767629076 cites W2029543903 @default.
• W2767629076 cites W2030147526 @default.
• W2767629076 cites W2037791777 @default.
• W2767629076 cites W2038761244 @default.
• W2767629076 cites W2040574649 @default.
• W2767629076 cites W2049404359 @default.
• W2767629076 cites W2053719062 @default.
• W2767629076 cites W2060519254 @default.
• W2767629076 cites W2060645147 @default.
• W2767629076 cites W2061509079 @default.
• W2767629076 cites W2070244173 @default.
• W2767629076 cites W2076993465 @default.
• W2767629076 cites W2081837827 @default.
• W2767629076 cites W2084847460 @default.
• W2767629076 cites W2090402561 @default.
• W2767629076 cites W2090477438 @default.
• W2767629076 cites W2094248609 @default.
• W2767629076 cites W2095477942 @default.
• W2767629076 cites W2102212449 @default.
• W2767629076 cites W2104135721 @default.
• W2767629076 cites W2112630596 @default.
• W2767629076 cites W2123106337 @default.
• W2767629076 cites W2126749888 @default.
• W2767629076 cites W2127298360 @default.
• W2767629076 cites W2133338739 @default.
• W2767629076 cites W2142136908 @default.
• W2767629076 cites W2149599746 @default.
• W2767629076 cites W2160961010 @default.
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• W2767629076 cites W2163039776 @default.
• W2767629076 cites W2169382112 @default.
• W2767629076 cites W2179438025 @default.
• W2767629076 cites W2179575365 @default.
• W2767629076 cites W2194756172 @default.
• W2767629076 cites W2231058499 @default.
• W2767629076 cites W2279350325 @default.
• W2767629076 cites W2289564378 @default.
• W2767629076 cites W2295921085 @default.
• W2767629076 cites W2333824284 @default.
• W2767629076 cites W2336440223 @default.
• W2767629076 cites W2345851252 @default.
• W2767629076 cites W2369050436 @default.
• W2767629076 cites W2406173703 @default.
• W2767629076 cites W2418046316 @default.
• W2767629076 cites W2466934234 @default.
• W2767629076 cites W2471513101 @default.
• W2767629076 cites W2512664017 @default.
• W2767629076 cites W2528543174 @default.
• W2767629076 cites W2547032004 @default.
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• W2767629076 cites W2589371597 @default.
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• W2767629076 cites W2751917967 @default.
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• W2767629076 cites W2766078734 @default.
• W2767629076 cites W322382484 @default.
• W2767629076 doi "https://doi.org/10.1101/215657" @default.
• W2767629076 hasPublicationYear "2017" @default.
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