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• W2767788891 abstract "Group II introns and non-LTR retrotransposons encode a phylogenetically related family of highly processive reverse transcriptases (RTs) that are essential for mobility and persistence of these retroelements. Recent crystallographic studies on members of this RT family have revealed that they are structurally distinct from the retroviral RTs that are typically used in biotechnology. However, quantitative, structure-guided analysis of processivity, efficiency, and accuracy of this alternate RT family has been lacking. Here, we characterize the processivity of a group II intron maturase RT from Eubacterium rectale ( E.r .), for which high-resolution structural information is available. We find that the E.r. maturase RT (MarathonRT) efficiently copies transcripts at least 10 kb in length and displays superior intrinsic RT processivity compared to commercial enzymes such as Superscript IV (SSIV). The elevated processivity of MarathonRT is at least partly mediated by a loop structure in the finger subdomain that acts as a steric guard (the α-loop). Additionally, we find that a positively charged secondary RNA binding site on the surface of the RT diminishes the primer utilization efficiency of the enzyme, and that reengineering of this surface enhances capabilities of the MarathonRT. Finally, using single-molecule sequencing, we show that the error frequency of MarathonRT is comparable to that of other high-performance RTs, such as SSIV, which were tested in parallel. Our results provide a structural framework for understanding the enhanced processivity of retroelement RTs, and they demonstrate the potential for engineering a powerful new generation of RT tools for application in biotechnology and research." @default.
• W2767788891 created "2017-11-17" @default.
• W2767788891 creator A5007909912 @default.
• W2767788891 creator A5048279362 @default.
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• W2767788891 date "2017-11-06" @default.
• W2767788891 modified "2023-10-14" @default.
• W2767788891 title "An ultraprocessive, accurate reverse transcriptase encoded by a metazoan group II intron" @default.
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• W2767788891 doi "https://doi.org/10.1261/rna.063479.117" @default.
• W2767788891 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5769746" @default.
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• W2767788891 hasPublicationYear "2017" @default.
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