Matches in SemOpenAlex for { <https://semopenalex.org/work/W2767848910> ?p ?o ?g. }
- W2767848910 endingPage "12861" @default.
- W2767848910 startingPage "12848" @default.
- W2767848910 abstract "Alternative end-joining (alt-EJ) repair of DNA double-strand breaks is associated with deletions, chromosome translocations, and genome instability. Alt-EJ frequently uses annealing of microhomologous sequences to tether broken ends. When accessible pre-existing microhomologies do not exist, we have postulated that new microhomologies can be created via limited DNA synthesis at secondary-structure forming sequences. This model, called synthesis-dependent microhomology-mediated end joining (SD-MMEJ), predicts that differences between DNA sequences near double-strand breaks should alter repair outcomes in predictable ways. To test this hypothesis, we injected plasmids with sequence variations flanking an I-SceI endonuclease recognition site into I-SceI expressing Drosophila embryos and used Illumina amplicon sequencing to compare repair junctions. As predicted by the model, we found that small changes in sequences near the I-SceI site had major impacts on the spectrum of repair junctions. Bioinformatic analyses suggest that these repair differences arise from transiently forming loops and hairpins within 30 nucleotides of the break. We also obtained evidence for 'trans SD-MMEJ,' involving at least two consecutive rounds of microhomology annealing and synthesis across the break site. These results highlight the importance of sequence context for alt-EJ repair and have important implications for genome editing and genome evolution." @default.
- W2767848910 created "2017-11-17" @default.
- W2767848910 creator A5000026759 @default.
- W2767848910 creator A5011855956 @default.
- W2767848910 creator A5024419190 @default.
- W2767848910 creator A5024799234 @default.
- W2767848910 creator A5045510214 @default.
- W2767848910 creator A5048642665 @default.
- W2767848910 creator A5061387346 @default.
- W2767848910 creator A5078987525 @default.
- W2767848910 date "2017-11-07" @default.
- W2767848910 modified "2023-10-15" @default.
- W2767848910 title "Secondary structure forming sequences drive SD-MMEJ repair of DNA double-strand breaks" @default.
- W2767848910 cites W1816802498 @default.
- W2767848910 cites W1822429854 @default.
- W2767848910 cites W1841331176 @default.
- W2767848910 cites W1920920544 @default.
- W2767848910 cites W1964097803 @default.
- W2767848910 cites W1965531809 @default.
- W2767848910 cites W1969262167 @default.
- W2767848910 cites W1976924852 @default.
- W2767848910 cites W1977644920 @default.
- W2767848910 cites W1978418405 @default.
- W2767848910 cites W1979240756 @default.
- W2767848910 cites W1987754718 @default.
- W2767848910 cites W2012425247 @default.
- W2767848910 cites W2015594699 @default.
- W2767848910 cites W2017253520 @default.
- W2767848910 cites W2020991778 @default.
- W2767848910 cites W2026557615 @default.
- W2767848910 cites W2036685117 @default.
- W2767848910 cites W2041286364 @default.
- W2767848910 cites W2041502642 @default.
- W2767848910 cites W2044916613 @default.
- W2767848910 cites W2048292104 @default.
- W2767848910 cites W2048342686 @default.
- W2767848910 cites W2053295911 @default.
- W2767848910 cites W2053844006 @default.
- W2767848910 cites W2056406429 @default.
- W2767848910 cites W2057902837 @default.
- W2767848910 cites W2060453248 @default.
- W2767848910 cites W2081673572 @default.
- W2767848910 cites W2093395725 @default.
- W2767848910 cites W2097627856 @default.
- W2767848910 cites W2100868970 @default.
- W2767848910 cites W2109316537 @default.
- W2767848910 cites W2113576659 @default.
- W2767848910 cites W2116311818 @default.
- W2767848910 cites W2123366092 @default.
- W2767848910 cites W2124013514 @default.
- W2767848910 cites W2132920733 @default.
- W2767848910 cites W2133432686 @default.
- W2767848910 cites W2135453449 @default.
- W2767848910 cites W2145583284 @default.
- W2767848910 cites W2146497194 @default.
- W2767848910 cites W2154822693 @default.
- W2767848910 cites W2162593589 @default.
- W2767848910 cites W2168518606 @default.
- W2767848910 cites W2195810723 @default.
- W2767848910 cites W2199055711 @default.
- W2767848910 cites W2203229763 @default.
- W2767848910 cites W2238730341 @default.
- W2767848910 cites W2254550535 @default.
- W2767848910 cites W2292151428 @default.
- W2767848910 cites W2319825622 @default.
- W2767848910 cites W2391227084 @default.
- W2767848910 cites W2407010699 @default.
- W2767848910 cites W2411273065 @default.
- W2767848910 cites W2429819149 @default.
- W2767848910 cites W2476261929 @default.
- W2767848910 cites W2484964216 @default.
- W2767848910 cites W2532894065 @default.
- W2767848910 cites W2555624605 @default.
- W2767848910 cites W2578064862 @default.
- W2767848910 cites W2580509112 @default.
- W2767848910 cites W2731920558 @default.
- W2767848910 cites W2734728768 @default.
- W2767848910 cites W919607714 @default.
- W2767848910 doi "https://doi.org/10.1093/nar/gkx1056" @default.
- W2767848910 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5728401" @default.
- W2767848910 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29121353" @default.
- W2767848910 hasPublicationYear "2017" @default.
- W2767848910 type Work @default.
- W2767848910 sameAs 2767848910 @default.
- W2767848910 citedByCount "29" @default.
- W2767848910 countsByYear W27678489102018 @default.
- W2767848910 countsByYear W27678489102019 @default.
- W2767848910 countsByYear W27678489102020 @default.
- W2767848910 countsByYear W27678489102021 @default.
- W2767848910 countsByYear W27678489102022 @default.
- W2767848910 countsByYear W27678489102023 @default.
- W2767848910 crossrefType "journal-article" @default.
- W2767848910 hasAuthorship W2767848910A5000026759 @default.
- W2767848910 hasAuthorship W2767848910A5011855956 @default.
- W2767848910 hasAuthorship W2767848910A5024419190 @default.
- W2767848910 hasAuthorship W2767848910A5024799234 @default.
- W2767848910 hasAuthorship W2767848910A5045510214 @default.
- W2767848910 hasAuthorship W2767848910A5048642665 @default.