FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2767891433> ?p ?o ?g. }

• W2767891433 endingPage "547" @default.
• W2767891433 startingPage "538" @default.
• W2767891433 abstract "Here, we demonstrate a powerful method to discriminate DNA mismatches at single-nucleotide resolution from 0 to 5 mismatches (χ0 to χ5) using Kelvin probe force microscopy (KPFM). Using our previously developed method, we quantified the surface potentials (SPs) of individual DNA-capped nanoparticles (DCNPs, ∼100 nm). On each DCNP, DNA hybridization occurs between ∼2200 immobilized probe DNA (pDNA) and target DNA with mismatches (tDNA, ∼80 nM). Thus, each DCNP used in the bioassay (each pDNA-tDNA interaction) corresponds to a single ensemble in which a large number of pDNA-tDNA interactions take place. Moreover, one KPFM image can scan at least dozens of ensembles, which allows statistical analysis (i.e., an ensemble average) of many bioassay cases (ensembles) under the same conditions. We found that as the χn increased from χ0 to χ5 in the tDNA, the average SP of dozens of ensembles (DCNPs) was attenuated owing to fewer hybridization events between the pDNA and the tDNA. Remarkably, the SP attenuation vs. the χn showed an inverse-linear correlation, albeit the equilibrium constant for DNA hybridization exponentially decreased asymptotically as the χn increased. In addition, we observed a cascade reaction at a 100-fold lower concentration of tDNA (∼0.8 nM); the average SP of DCNPs exhibited no significant decrease but rather split into two separate states (no-hybridization vs. full-hybridization). Compared to complementary tDNA (i.e., χ0), the ratio of no-hybridization/full-hybridization within a given set of DCNPs became ∼1.6 times higher in the presence of tDNA with single mismatches (i.e., χ1). The results imply that our method opens new avenues not only in the research on the DNA hybridization mechanism in the presence of DNA mismatches but also in the development of a robust technology for DNA mismatch detection." @default.
• W2767891433 created "2017-11-17" @default.
• W2767891433 creator A5006029344 @default.
• W2767891433 creator A5006547542 @default.
• W2767891433 creator A5007291907 @default.
• W2767891433 creator A5010172715 @default.
• W2767891433 creator A5025781007 @default.
• W2767891433 creator A5026334171 @default.
• W2767891433 creator A5050946440 @default.
• W2767891433 creator A5053318183 @default.
• W2767891433 creator A5063300940 @default.
• W2767891433 creator A5074254878 @default.
• W2767891433 creator A5079197821 @default.
• W2767891433 creator A5084313587 @default.
• W2767891433 date "2018-01-01" @default.
• W2767891433 modified "2023-10-16" @default.
• W2767891433 title "Identifying DNA mismatches at single-nucleotide resolution by probing individual surface potentials of DNA-capped nanoparticles" @default.
• W2767891433 cites W1631110814 @default.
• W2767891433 cites W1969947375 @default.
• W2767891433 cites W1974646727 @default.
• W2767891433 cites W1976707311 @default.
• W2767891433 cites W1977866604 @default.
• W2767891433 cites W1988374960 @default.
• W2767891433 cites W1990607414 @default.
• W2767891433 cites W1994316057 @default.
• W2767891433 cites W1997531346 @default.
• W2767891433 cites W2007570983 @default.
• W2767891433 cites W2017130266 @default.
• W2767891433 cites W2018152432 @default.
• W2767891433 cites W2023568503 @default.
• W2767891433 cites W2023936383 @default.
• W2767891433 cites W2032711662 @default.
• W2767891433 cites W2041970364 @default.
• W2767891433 cites W2043793103 @default.
• W2767891433 cites W2048926712 @default.
• W2767891433 cites W2054972712 @default.
• W2767891433 cites W2057800132 @default.
• W2767891433 cites W2062802178 @default.
• W2767891433 cites W2066467970 @default.
• W2767891433 cites W2071373898 @default.
• W2767891433 cites W2071813506 @default.
• W2767891433 cites W2072856841 @default.
• W2767891433 cites W2077640472 @default.
• W2767891433 cites W2082248073 @default.
• W2767891433 cites W2082277797 @default.
• W2767891433 cites W2085062917 @default.
• W2767891433 cites W2085475044 @default.
• W2767891433 cites W2110146951 @default.
• W2767891433 cites W2117228527 @default.
• W2767891433 cites W2125789330 @default.
• W2767891433 cites W2140167371 @default.
• W2767891433 cites W2144400286 @default.
• W2767891433 cites W2144689560 @default.
• W2767891433 cites W2148332776 @default.
• W2767891433 cites W2150111967 @default.
• W2767891433 cites W2165686339 @default.
• W2767891433 cites W2168864790 @default.
• W2767891433 cites W2170347395 @default.
• W2767891433 cites W2278203278 @default.
• W2767891433 cites W2343148347 @default.
• W2767891433 cites W2401811918 @default.
• W2767891433 cites W4253361987 @default.
• W2767891433 doi "https://doi.org/10.1039/c7nr05250b" @default.
• W2767891433 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29167849" @default.
• W2767891433 hasPublicationYear "2018" @default.
• W2767891433 type Work @default.
• W2767891433 sameAs 2767891433 @default.
• W2767891433 citedByCount "11" @default.
• W2767891433 countsByYear W27678914332018 @default.
• W2767891433 countsByYear W27678914332019 @default.
• W2767891433 countsByYear W27678914332020 @default.
• W2767891433 countsByYear W27678914332021 @default.
• W2767891433 countsByYear W27678914332022 @default.
• W2767891433 crossrefType "journal-article" @default.
• W2767891433 hasAuthorship W2767891433A5006029344 @default.
• W2767891433 hasAuthorship W2767891433A5006547542 @default.
• W2767891433 hasAuthorship W2767891433A5007291907 @default.
• W2767891433 hasAuthorship W2767891433A5010172715 @default.
• W2767891433 hasAuthorship W2767891433A5025781007 @default.
• W2767891433 hasAuthorship W2767891433A5026334171 @default.
• W2767891433 hasAuthorship W2767891433A5050946440 @default.
• W2767891433 hasAuthorship W2767891433A5053318183 @default.
• W2767891433 hasAuthorship W2767891433A5063300940 @default.
• W2767891433 hasAuthorship W2767891433A5074254878 @default.
• W2767891433 hasAuthorship W2767891433A5079197821 @default.
• W2767891433 hasAuthorship W2767891433A5084313587 @default.
• W2767891433 hasConcept C102230213 @default.
• W2767891433 hasConcept C104317684 @default.
• W2767891433 hasConcept C104488531 @default.
• W2767891433 hasConcept C113196181 @default.
• W2767891433 hasConcept C12554922 @default.
• W2767891433 hasConcept C130197536 @default.
• W2767891433 hasConcept C137390916 @default.
• W2767891433 hasConcept C153911025 @default.
• W2767891433 hasConcept C160136775 @default.
• W2767891433 hasConcept C171250308 @default.
• W2767891433 hasConcept C185592680 @default.
• W2767891433 hasConcept C186060115 @default.

• next