Matches in SemOpenAlex for { <https://semopenalex.org/work/W2767909722> ?p ?o ?g. }
- W2767909722 abstract "The aim of the present study was to investigate the potential of bone mesenchymal stem cells (BMSCs) treated with a combination of vascular endothelial growth factor (VEGF) and bone morphogenetic protein‑6 (BMP‑6) genes for the treatment of avascular necrosis of the femoral head (ANFH). Rat BMSCs were isolated and purified using a density gradient centrifugation method. The purity and characteristics of the BMSCs were detected by cell surface antigens identification using flow cytometry. The experimental groups were administered with one of the following adeno‑associated virus (AAV) vector constructs: AAV‑green fluorescent protein (AAV‑GFP), AAV‑BMP‑6, AAV‑VEGF or AAV‑VEGF‑BMP‑6. The expression of VEGF and BMP‑6 was detected by reverse transcription‑quantitative polymerase chain reaction, western blotting and ELISA assays. The effects of VEGF and BMP‑6 on BMSCs were evaluated by angiogenic and osteogenic assays. The transfected BMSCs were combined with a biomimetic synthetic scaffold poly lactide‑co‑glycolide (PLAGA) and they were then subcutaneously implanted into nude mice. After four weeks, the implants were analyzed with histology and subsequent immunostaining to evaluate the effects of BMSCs on blood vessel and bone formation in vivo. In the AAV‑VEGF‑BMP‑6 group, the expression levels of VEGF and BMP‑6 were significantly increased and human umbilical vein endothelial cells tube formation was significantly enhanced compared with other groups. Capillaries and bone formation in the AAV‑VEGF‑BMP‑6 group was significantly higher compared with the other groups. The results of the present study suggest that BMSCs expressing both VEGF and BMP‑6 induce an increase in blood vessels and bone formation, which provides theoretical support for ANFH gene therapy." @default.
- W2767909722 created "2017-11-17" @default.
- W2767909722 creator A5022097699 @default.
- W2767909722 creator A5033837381 @default.
- W2767909722 creator A5070135290 @default.
- W2767909722 creator A5072964111 @default.
- W2767909722 creator A5088413875 @default.
- W2767909722 creator A5089392461 @default.
- W2767909722 date "2017-11-07" @default.
- W2767909722 modified "2023-10-02" @default.
- W2767909722 title "Bone mesenchymal stem cells co‑expressing VEGF and BMP‑6 genes to combat avascular necrosis of the femoral head" @default.
- W2767909722 cites W1492279074 @default.
- W2767909722 cites W1503630626 @default.
- W2767909722 cites W1583699618 @default.
- W2767909722 cites W1595761687 @default.
- W2767909722 cites W1956517332 @default.
- W2767909722 cites W1958248902 @default.
- W2767909722 cites W1964052526 @default.
- W2767909722 cites W1972801007 @default.
- W2767909722 cites W1977428529 @default.
- W2767909722 cites W1977642962 @default.
- W2767909722 cites W1982479589 @default.
- W2767909722 cites W2000330544 @default.
- W2767909722 cites W2006174806 @default.
- W2767909722 cites W2008976766 @default.
- W2767909722 cites W2019777614 @default.
- W2767909722 cites W2020792282 @default.
- W2767909722 cites W2022161423 @default.
- W2767909722 cites W2024954960 @default.
- W2767909722 cites W2026772281 @default.
- W2767909722 cites W2028846632 @default.
- W2767909722 cites W2033016180 @default.
- W2767909722 cites W2035829944 @default.
- W2767909722 cites W2038808891 @default.
- W2767909722 cites W2042373581 @default.
- W2767909722 cites W2059669211 @default.
- W2767909722 cites W2060350329 @default.
- W2767909722 cites W2061124209 @default.
- W2767909722 cites W2065694620 @default.
- W2767909722 cites W2067365522 @default.
- W2767909722 cites W2067548759 @default.
- W2767909722 cites W2074677451 @default.
- W2767909722 cites W2075533939 @default.
- W2767909722 cites W2088459489 @default.
- W2767909722 cites W2088840362 @default.
- W2767909722 cites W2089788551 @default.
- W2767909722 cites W2089863521 @default.
- W2767909722 cites W2092987783 @default.
- W2767909722 cites W2104711663 @default.
- W2767909722 cites W2107277218 @default.
- W2767909722 cites W2123640323 @default.
- W2767909722 cites W2140557153 @default.
- W2767909722 cites W2145591890 @default.
- W2767909722 cites W2158847377 @default.
- W2767909722 cites W2159999899 @default.
- W2767909722 cites W2165715446 @default.
- W2767909722 cites W2167575455 @default.
- W2767909722 cites W2168789685 @default.
- W2767909722 cites W2169932958 @default.
- W2767909722 cites W2171802389 @default.
- W2767909722 cites W2185873440 @default.
- W2767909722 cites W2189534812 @default.
- W2767909722 cites W2192080449 @default.
- W2767909722 cites W2316244478 @default.
- W2767909722 cites W2493763734 @default.
- W2767909722 doi "https://doi.org/10.3892/etm.2017.5455" @default.
- W2767909722 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5772743" @default.
- W2767909722 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29399103" @default.
- W2767909722 hasPublicationYear "2017" @default.
- W2767909722 type Work @default.
- W2767909722 sameAs 2767909722 @default.
- W2767909722 citedByCount "9" @default.
- W2767909722 countsByYear W27679097222019 @default.
- W2767909722 countsByYear W27679097222020 @default.
- W2767909722 countsByYear W27679097222021 @default.
- W2767909722 countsByYear W27679097222022 @default.
- W2767909722 countsByYear W27679097222023 @default.
- W2767909722 crossrefType "journal-article" @default.
- W2767909722 hasAuthorship W2767909722A5022097699 @default.
- W2767909722 hasAuthorship W2767909722A5033837381 @default.
- W2767909722 hasAuthorship W2767909722A5070135290 @default.
- W2767909722 hasAuthorship W2767909722A5072964111 @default.
- W2767909722 hasAuthorship W2767909722A5088413875 @default.
- W2767909722 hasAuthorship W2767909722A5089392461 @default.
- W2767909722 hasBestOaLocation W27679097221 @default.
- W2767909722 hasConcept C142724271 @default.
- W2767909722 hasConcept C153911025 @default.
- W2767909722 hasConcept C167734588 @default.
- W2767909722 hasConcept C185592680 @default.
- W2767909722 hasConcept C198826908 @default.
- W2767909722 hasConcept C202751555 @default.
- W2767909722 hasConcept C2777025900 @default.
- W2767909722 hasConcept C2780394083 @default.
- W2767909722 hasConcept C30352789 @default.
- W2767909722 hasConcept C502942594 @default.
- W2767909722 hasConcept C55493867 @default.
- W2767909722 hasConcept C71924100 @default.
- W2767909722 hasConcept C86803240 @default.
- W2767909722 hasConceptScore W2767909722C142724271 @default.
- W2767909722 hasConceptScore W2767909722C153911025 @default.