Matches in SemOpenAlex for { <https://semopenalex.org/work/W2767936334> ?p ?o ?g. }
- W2767936334 endingPage "236" @default.
- W2767936334 startingPage "219" @default.
- W2767936334 abstract "The nucleocapsid (NC) is an N-terminal protein derived from the HIV-1 Gag precursor polyprotein, pr55 Gag . NC possesses key functions at several pivotal stages of viral replication. For example, an interaction between NC and the host double-stranded RNA-binding protein Staufen1 was shown to regulate several steps in the viral replication cycle, such as Gag multimerization and genomic RNA encapsidation. In this work, we observed that the overexpression of NC leads to the induction of stress granule (SG) assembly. NC-mediated SG assembly was unique as it was resistant to the SG blockade imposed by the HIV-1 capsid (CA), as shown in earlier work. NC also reduced host cell mRNA translation, as judged by a puromycylation assay of de novo synthesized proteins, and this was recapitulated in polysome profile analyses. Virus production was also found to be significantly reduced. Finally, Staufen1 expression completely rescued the blockade to NC-mediated SG assembly, global mRNA translation as well as virus production. NC expression also resulted in the phosphorylation of protein kinase R (PKR) and eIF2α, and this was inhibited with Staufen1 coexpression. This work sheds light on an unexpected function of NC in host cell translation. A comprehensive understanding of the molecular mechanisms by which a fine balance of the HIV-1 structural proteins NC and CA act in concert with host proteins such as Staufen1 to modulate the host stress response will aid in the development of new antiviral therapeutics." @default.
- W2767936334 created "2017-11-17" @default.
- W2767936334 creator A5017267110 @default.
- W2767936334 creator A5029351442 @default.
- W2767936334 creator A5074852430 @default.
- W2767936334 creator A5082296960 @default.
- W2767936334 creator A5086850461 @default.
- W2767936334 creator A5089597641 @default.
- W2767936334 date "2017-11-10" @default.
- W2767936334 modified "2023-10-10" @default.
- W2767936334 title "HIV-1 NC-induced stress granule assembly and translation arrest are inhibited by the dsRNA binding protein Staufen1" @default.
- W2767936334 cites W112912425 @default.
- W2767936334 cites W1602204726 @default.
- W2767936334 cites W1641947900 @default.
- W2767936334 cites W1750901338 @default.
- W2767936334 cites W1834008389 @default.
- W2767936334 cites W1837392183 @default.
- W2767936334 cites W1969836275 @default.
- W2767936334 cites W1978547782 @default.
- W2767936334 cites W1983878572 @default.
- W2767936334 cites W1985581648 @default.
- W2767936334 cites W1985665571 @default.
- W2767936334 cites W1987239216 @default.
- W2767936334 cites W1992496728 @default.
- W2767936334 cites W1993319890 @default.
- W2767936334 cites W1997408440 @default.
- W2767936334 cites W2000284725 @default.
- W2767936334 cites W2002445611 @default.
- W2767936334 cites W2003540996 @default.
- W2767936334 cites W2005465321 @default.
- W2767936334 cites W2007400675 @default.
- W2767936334 cites W2009378735 @default.
- W2767936334 cites W2012718182 @default.
- W2767936334 cites W2019726061 @default.
- W2767936334 cites W2022408275 @default.
- W2767936334 cites W2022662073 @default.
- W2767936334 cites W2025483501 @default.
- W2767936334 cites W2026067675 @default.
- W2767936334 cites W2036547488 @default.
- W2767936334 cites W2036853011 @default.
- W2767936334 cites W2037513168 @default.
- W2767936334 cites W2040024486 @default.
- W2767936334 cites W2040721528 @default.
- W2767936334 cites W2042676429 @default.
- W2767936334 cites W2060655204 @default.
- W2767936334 cites W2062477253 @default.
- W2767936334 cites W2062656304 @default.
- W2767936334 cites W2065023490 @default.
- W2767936334 cites W2067928523 @default.
- W2767936334 cites W2068151486 @default.
- W2767936334 cites W2069440293 @default.
- W2767936334 cites W2074882944 @default.
- W2767936334 cites W2076495609 @default.
- W2767936334 cites W2079838847 @default.
- W2767936334 cites W2080793236 @default.
- W2767936334 cites W2093117575 @default.
- W2767936334 cites W2095109616 @default.
- W2767936334 cites W2097405423 @default.
- W2767936334 cites W2099481042 @default.
- W2767936334 cites W2105205198 @default.
- W2767936334 cites W2108736984 @default.
- W2767936334 cites W2115094328 @default.
- W2767936334 cites W2116438422 @default.
- W2767936334 cites W2117867057 @default.
- W2767936334 cites W2119977018 @default.
- W2767936334 cites W2127025250 @default.
- W2767936334 cites W2128244329 @default.
- W2767936334 cites W2130784499 @default.
- W2767936334 cites W2131122524 @default.
- W2767936334 cites W2133919090 @default.
- W2767936334 cites W2137581965 @default.
- W2767936334 cites W2138267133 @default.
- W2767936334 cites W2149180811 @default.
- W2767936334 cites W2149225898 @default.
- W2767936334 cites W2149541702 @default.
- W2767936334 cites W2150176648 @default.
- W2767936334 cites W2150538842 @default.
- W2767936334 cites W2150860983 @default.
- W2767936334 cites W2151942229 @default.
- W2767936334 cites W2158264786 @default.
- W2767936334 cites W2158420057 @default.
- W2767936334 cites W2162592749 @default.
- W2767936334 cites W2165761157 @default.
- W2767936334 cites W2166219890 @default.
- W2767936334 cites W2293962087 @default.
- W2767936334 cites W2311204218 @default.
- W2767936334 cites W2321109448 @default.
- W2767936334 cites W2324264095 @default.
- W2767936334 cites W2338117252 @default.
- W2767936334 cites W2465340248 @default.
- W2767936334 cites W2562654073 @default.
- W2767936334 cites W2573507107 @default.
- W2767936334 cites W2734489868 @default.
- W2767936334 cites W4247037821 @default.
- W2767936334 cites W61418099 @default.
- W2767936334 doi "https://doi.org/10.1261/rna.064618.117" @default.
- W2767936334 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5769749" @default.
- W2767936334 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29127210" @default.