FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2768077089> ?p ?o ?g. }

• W2768077089 abstract "ABSTRACT For decades, in situ hybridization methods have been essential tools for studies of vertebrate development and disease, as they enable qualitative analyses of mRNA expression in an anatomical context. Quantitative mRNA analyses typically sacrifice the anatomy, relying on embryo microdissection, dissociation, cell sorting and/or homogenization. Here, we eliminate the trade-off between quantitation and anatomical context, using quantitative in situ hybridization chain reaction (qHCR) to perform accurate and precise relative quantitation of mRNA expression with subcellular resolution within whole-mount vertebrate embryos. Gene expression can be queried in two directions: read-out from anatomical space to expression space reveals co-expression relationships in selected regions of the specimen; conversely, read-in from multidimensional expression space to anatomical space reveals those anatomical locations in which selected gene co-expression relationships occur. As we demonstrate by examining gene circuits underlying somitogenesis, quantitative read-out and read-in analyses provide the strengths of flow cytometry expression analyses, but by preserving subcellular anatomical context, they enable bi-directional queries that open a new era for in situ hybridization." @default.
• W2768077089 created "2017-11-17" @default.
• W2768077089 creator A5021270248 @default.
• W2768077089 creator A5047064481 @default.
• W2768077089 creator A5049814739 @default.
• W2768077089 creator A5077536847 @default.
• W2768077089 date "2018-01-01" @default.
• W2768077089 modified "2023-10-15" @default.
• W2768077089 title "Multidimensional quantitative analysis of mRNA expression within intact vertebrate embryos" @default.
• W2768077089 cites W1555420746 @default.
• W2768077089 cites W1928939008 @default.
• W2768077089 cites W1964372716 @default.
• W2768077089 cites W1970193155 @default.
• W2768077089 cites W1985207173 @default.
• W2768077089 cites W1985287227 @default.
• W2768077089 cites W1986506040 @default.
• W2768077089 cites W1987318612 @default.
• W2768077089 cites W1991777500 @default.
• W2768077089 cites W1995362898 @default.
• W2768077089 cites W2001309634 @default.
• W2768077089 cites W2014878330 @default.
• W2768077089 cites W2014908429 @default.
• W2768077089 cites W2019490156 @default.
• W2768077089 cites W2022731947 @default.
• W2768077089 cites W2023068246 @default.
• W2768077089 cites W2032411179 @default.
• W2768077089 cites W2038359397 @default.
• W2768077089 cites W2044777133 @default.
• W2768077089 cites W2049136149 @default.
• W2768077089 cites W2053188090 @default.
• W2768077089 cites W2065827303 @default.
• W2768077089 cites W2066649475 @default.
• W2768077089 cites W2067861226 @default.
• W2768077089 cites W2068881679 @default.
• W2768077089 cites W2073271124 @default.
• W2768077089 cites W2086629747 @default.
• W2768077089 cites W2092811395 @default.
• W2768077089 cites W2094844506 @default.
• W2768077089 cites W2102080233 @default.
• W2768077089 cites W2102910500 @default.
• W2768077089 cites W2111928315 @default.
• W2768077089 cites W2126862456 @default.
• W2768077089 cites W2135765390 @default.
• W2768077089 cites W2143991868 @default.
• W2768077089 cites W2148304424 @default.
• W2768077089 cites W2149552563 @default.
• W2768077089 cites W2157248035 @default.
• W2768077089 cites W2163383148 @default.
• W2768077089 cites W2208796000 @default.
• W2768077089 cites W2316095770 @default.
• W2768077089 cites W2417418204 @default.
• W2768077089 cites W2418574046 @default.
• W2768077089 cites W242008281 @default.
• W2768077089 cites W2465973416 @default.
• W2768077089 cites W2467652252 @default.
• W2768077089 cites W2527833694 @default.
• W2768077089 cites W3212157965 @default.
• W2768077089 cites W4256266823 @default.
• W2768077089 cites W4297628970 @default.
• W2768077089 cites W48566380 @default.
• W2768077089 doi "https://doi.org/10.1242/dev.156869" @default.
• W2768077089 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5825878" @default.
• W2768077089 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29311262" @default.
• W2768077089 hasPublicationYear "2018" @default.
• W2768077089 type Work @default.
• W2768077089 sameAs 2768077089 @default.
• W2768077089 citedByCount "49" @default.
• W2768077089 countsByYear W27680770892018 @default.
• W2768077089 countsByYear W27680770892019 @default.
• W2768077089 countsByYear W27680770892020 @default.
• W2768077089 countsByYear W27680770892021 @default.
• W2768077089 countsByYear W27680770892022 @default.
• W2768077089 countsByYear W27680770892023 @default.
• W2768077089 crossrefType "journal-article" @default.
• W2768077089 hasAuthorship W2768077089A5021270248 @default.
• W2768077089 hasAuthorship W2768077089A5047064481 @default.
• W2768077089 hasAuthorship W2768077089A5049814739 @default.
• W2768077089 hasAuthorship W2768077089A5077536847 @default.
• W2768077089 hasBestOaLocation W27680770891 @default.
• W2768077089 hasConcept C104317684 @default.
• W2768077089 hasConcept C150194340 @default.
• W2768077089 hasConcept C151730666 @default.
• W2768077089 hasConcept C16693951 @default.
• W2768077089 hasConcept C18431079 @default.
• W2768077089 hasConcept C196843134 @default.
• W2768077089 hasConcept C2778961482 @default.
• W2768077089 hasConcept C2779018604 @default.
• W2768077089 hasConcept C2779343474 @default.
• W2768077089 hasConcept C54355233 @default.
• W2768077089 hasConcept C70721500 @default.
• W2768077089 hasConcept C81439078 @default.
• W2768077089 hasConcept C86803240 @default.
• W2768077089 hasConcept C87073359 @default.
• W2768077089 hasConcept C95444343 @default.
• W2768077089 hasConceptScore W2768077089C104317684 @default.
• W2768077089 hasConceptScore W2768077089C150194340 @default.
• W2768077089 hasConceptScore W2768077089C151730666 @default.
• W2768077089 hasConceptScore W2768077089C16693951 @default.
• W2768077089 hasConceptScore W2768077089C18431079 @default.
• W2768077089 hasConceptScore W2768077089C196843134 @default.

• next