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• W2768263485 abstract "During transcription, the nascent transcript behind an elongating RNA polymerase (RNAP) can invade the DNA duplex and hybridize with the complementary DNA template strand, generating a three-stranded R-loop structure, composed of an RNA:DNA duplex and an unpaired non-template DNA strand. R-loops can be strongly associated with actively transcribed loci by all RNAPs including the mitochondrial RNA polymerase (mtRNAP). In this chapter, we describe two protocols for the detection of RNA:DNA hybrids in living budding yeast cells, one that uses conventional chromatin immunoprecipitation (ChIP-qPCR) and one that uses DNA:RNA immunoprecipitation (DRIP-qPCR). Both protocols make use of the S9.6 antibody, which is believed to recognize the intermediate A/B helical RNA:DNA duplex conformation, with no sequence specificity." @default.
• W2768263485 created "2017-12-04" @default.
• W2768263485 creator A5010467813 @default.
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• W2768263485 date "2017-11-25" @default.
• W2768263485 modified "2023-09-23" @default.
• W2768263485 title "Immunoprecipitation of RNA:DNA Hybrids from Budding Yeast" @default.
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• W2768263485 doi "https://doi.org/10.1007/978-1-4939-7459-7_8" @default.
• W2768263485 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29177737" @default.
• W2768263485 hasPublicationYear "2017" @default.
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