FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2768455291> ?p ?o ?g. }

• W2768455291 endingPage "e0188130" @default.
• W2768455291 startingPage "e0188130" @default.
• W2768455291 abstract "The 2-methylcitric acid cycle (2-MCC) is a common route of propionate catabolism in microorganisms. In Salmonella enterica, the prpBCDE operon encodes most of the 2-MCC enzymes. In other organisms, e.g., Shewanella oneidensis MR-1, two genes, acnD and prpF replace prpD, which encodes 2-methylcitrate dehydratase. We showed that together, S. oneidensis AcnD and PrpF (SoAcnD, SoPrpF) compensated for the absence of PrpD in a S. enterica prpD strain. We also showed that SoAcnD had 2-methylcitrate dehydratase activity and that PrpF has aconitate isomerase activity. Here we report in vitro evidence that the product of the SoAcnD reaction is an isomer of 2-methyl-cis-aconitate (2-MCA], the product of the SePrpD reaction. We show that the SoPrpF protein isomerizes the product of the AcnD reaction into the PrpD product (2-MCA], a known substrate of the housekeeping aconitase (AcnB]. Given that SoPrpF is an isomerase, that SoAcnD is a dehydratase, and the results from in vivo and in vitro experiments reported here, it is likely that 4-methylaconitate is the product of the AcnD enzyme. Results from in vivo studies using a S. enterica prpD strain show that SoPrpF variants with substitutions of residues K73 or C107 failed to support growth with propionate as the sole source of carbon and energy. High-resolution (1.22 Å) three-dimensional crystal structures of PrpFK73E in complex with trans-aconitate or malonate provide insights into the mechanism of catalysis of the wild-type protein." @default.
• W2768455291 created "2017-12-04" @default.
• W2768455291 creator A5008115009 @default.
• W2768455291 creator A5010083940 @default.
• W2768455291 creator A5036537405 @default.
• W2768455291 creator A5056279286 @default.
• W2768455291 creator A5074949575 @default.
• W2768455291 date "2017-11-16" @default.
• W2768455291 modified "2023-09-27" @default.
• W2768455291 title "The PrpF protein of Shewanella oneidensis MR-1 catalyzes the isomerization of 2-methyl-cis-aconitate during the catabolism of propionate via the AcnD-dependent 2-methylcitric acid cycle" @default.
• W2768455291 cites W1489331825 @default.
• W2768455291 cites W1511483705 @default.
• W2768455291 cites W1539796472 @default.
• W2768455291 cites W1547139981 @default.
• W2768455291 cites W1560139137 @default.
• W2768455291 cites W1562503516 @default.
• W2768455291 cites W1592463355 @default.
• W2768455291 cites W1943327251 @default.
• W2768455291 cites W1949125327 @default.
• W2768455291 cites W1965614823 @default.
• W2768455291 cites W2009883823 @default.
• W2768455291 cites W2012985142 @default.
• W2768455291 cites W2029928922 @default.
• W2768455291 cites W2031142633 @default.
• W2768455291 cites W2032303370 @default.
• W2768455291 cites W2033507368 @default.
• W2768455291 cites W2034672527 @default.
• W2768455291 cites W2038840577 @default.
• W2768455291 cites W2049163769 @default.
• W2768455291 cites W2055791633 @default.
• W2768455291 cites W2063425168 @default.
• W2768455291 cites W2069952742 @default.
• W2768455291 cites W2071038504 @default.
• W2768455291 cites W2072234490 @default.
• W2768455291 cites W2090530080 @default.
• W2768455291 cites W2090837980 @default.
• W2768455291 cites W2096101547 @default.
• W2768455291 cites W2112942905 @default.
• W2768455291 cites W2114557611 @default.
• W2768455291 cites W2117463105 @default.
• W2768455291 cites W2138020404 @default.
• W2768455291 cites W2141417334 @default.
• W2768455291 cites W2142299594 @default.
• W2768455291 cites W2144081223 @default.
• W2768455291 cites W2144998676 @default.
• W2768455291 cites W2152349129 @default.
• W2768455291 cites W2154292013 @default.
• W2768455291 cites W2155503404 @default.
• W2768455291 cites W2159283574 @default.
• W2768455291 cites W2160084442 @default.
• W2768455291 cites W2160508716 @default.
• W2768455291 cites W2163341755 @default.
• W2768455291 cites W2180229411 @default.
• W2768455291 cites W4236772064 @default.
• W2768455291 cites W2092144749 @default.
• W2768455291 doi "https://doi.org/10.1371/journal.pone.0188130" @default.
• W2768455291 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5690661" @default.
• W2768455291 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29145506" @default.
• W2768455291 hasPublicationYear "2017" @default.
• W2768455291 type Work @default.
• W2768455291 sameAs 2768455291 @default.
• W2768455291 citedByCount "9" @default.
• W2768455291 countsByYear W27684552912018 @default.
• W2768455291 countsByYear W27684552912019 @default.
• W2768455291 countsByYear W27684552912020 @default.
• W2768455291 countsByYear W27684552912021 @default.
• W2768455291 countsByYear W27684552912023 @default.
• W2768455291 crossrefType "journal-article" @default.
• W2768455291 hasAuthorship W2768455291A5008115009 @default.
• W2768455291 hasAuthorship W2768455291A5010083940 @default.
• W2768455291 hasAuthorship W2768455291A5036537405 @default.
• W2768455291 hasAuthorship W2768455291A5056279286 @default.
• W2768455291 hasAuthorship W2768455291A5074949575 @default.
• W2768455291 hasBestOaLocation W27684552911 @default.
• W2768455291 hasConcept C106302636 @default.
• W2768455291 hasConcept C181199279 @default.
• W2768455291 hasConcept C185592680 @default.
• W2768455291 hasConcept C200886947 @default.
• W2768455291 hasConcept C2780014933 @default.
• W2768455291 hasConcept C2781170380 @default.
• W2768455291 hasConcept C2781264208 @default.
• W2768455291 hasConcept C2781374117 @default.
• W2768455291 hasConcept C523546767 @default.
• W2768455291 hasConcept C54355233 @default.
• W2768455291 hasConcept C55493867 @default.
• W2768455291 hasConcept C86803240 @default.
• W2768455291 hasConceptScore W2768455291C106302636 @default.
• W2768455291 hasConceptScore W2768455291C181199279 @default.
• W2768455291 hasConceptScore W2768455291C185592680 @default.
• W2768455291 hasConceptScore W2768455291C200886947 @default.
• W2768455291 hasConceptScore W2768455291C2780014933 @default.
• W2768455291 hasConceptScore W2768455291C2781170380 @default.
• W2768455291 hasConceptScore W2768455291C2781264208 @default.
• W2768455291 hasConceptScore W2768455291C2781374117 @default.
• W2768455291 hasConceptScore W2768455291C523546767 @default.
• W2768455291 hasConceptScore W2768455291C54355233 @default.
• W2768455291 hasConceptScore W2768455291C55493867 @default.
• W2768455291 hasConceptScore W2768455291C86803240 @default.

• next