Matches in SemOpenAlex for { <https://semopenalex.org/work/W2768535483> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2768535483 endingPage "179" @default.
- W2768535483 startingPage "174" @default.
- W2768535483 abstract "The middle cerebral artery occlusion (MCAO) model in rodents has been widely used as model for studying brain ischemic stroke. TTC (2,3,5-triphenyltetrazolium chloride) staining in fresh tissues is used to evaluate the size of the infarct in MCAO model, and TTC-stained brain tissues are considered to be possible to bring a damage to the anatomical structure of neuronal cells and unsuitable for immunofluorescence analyses of cytology, and discarded after evaluation of infarct volume. Another group of models with in vivo fixation was required to the pathological or histological analyses of the infarct brains, which lead to double the numbers of animals in researches. However, some evidences indicate that if we properly optimized staining protocol, TTC-stained brain tissues might be suitable for cytological analyses. In this work, we have optimized the immunofluorescent staining methods of TTC-stained brain slices, and found that TTC-stained brain tissues are suitable for quantitative and qualitative analyses of microglia, astrocytes and neuroblasts, the morphology of theses cell were nearly identical to the in-vivo fixed models. Our optimized-protocol provide two advantages over traditional methods one of them is providing the precise the infarct region, which reduces the differences within groups, the other one is decreasing the total number of animals in research dramatically." @default.
- W2768535483 created "2017-12-04" @default.
- W2768535483 creator A5017766424 @default.
- W2768535483 creator A5027475930 @default.
- W2768535483 creator A5090015051 @default.
- W2768535483 date "2018-01-01" @default.
- W2768535483 modified "2023-10-02" @default.
- W2768535483 title "Use of 2,3,5-triphenyltetrazolium chloride-stained brain tissues for immunofluorescence analyses after focal cerebral ischemia in rats" @default.
- W2768535483 cites W1969824218 @default.
- W2768535483 cites W1974483939 @default.
- W2768535483 cites W1976017263 @default.
- W2768535483 cites W1987666229 @default.
- W2768535483 cites W2000427241 @default.
- W2768535483 cites W2019294123 @default.
- W2768535483 cites W2023041512 @default.
- W2768535483 cites W2046297960 @default.
- W2768535483 cites W2053674513 @default.
- W2768535483 cites W2063341946 @default.
- W2768535483 cites W2065219991 @default.
- W2768535483 cites W2067267561 @default.
- W2768535483 cites W2071420776 @default.
- W2768535483 cites W2074661552 @default.
- W2768535483 cites W2083647133 @default.
- W2768535483 cites W2084995170 @default.
- W2768535483 cites W2087297677 @default.
- W2768535483 cites W2091740143 @default.
- W2768535483 cites W2095885953 @default.
- W2768535483 cites W2154676941 @default.
- W2768535483 cites W2160779535 @default.
- W2768535483 cites W2168272960 @default.
- W2768535483 cites W4229512970 @default.
- W2768535483 doi "https://doi.org/10.1016/j.prp.2017.11.016" @default.
- W2768535483 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29254786" @default.
- W2768535483 hasPublicationYear "2018" @default.
- W2768535483 type Work @default.
- W2768535483 sameAs 2768535483 @default.
- W2768535483 citedByCount "14" @default.
- W2768535483 countsByYear W27685354832019 @default.
- W2768535483 countsByYear W27685354832021 @default.
- W2768535483 countsByYear W27685354832022 @default.
- W2768535483 countsByYear W27685354832023 @default.
- W2768535483 crossrefType "journal-article" @default.
- W2768535483 hasAuthorship W2768535483A5017766424 @default.
- W2768535483 hasAuthorship W2768535483A5027475930 @default.
- W2768535483 hasAuthorship W2768535483A5090015051 @default.
- W2768535483 hasConcept C104317684 @default.
- W2768535483 hasConcept C126322002 @default.
- W2768535483 hasConcept C142724271 @default.
- W2768535483 hasConcept C146249460 @default.
- W2768535483 hasConcept C150903083 @default.
- W2768535483 hasConcept C159654299 @default.
- W2768535483 hasConcept C203014093 @default.
- W2768535483 hasConcept C207001950 @default.
- W2768535483 hasConcept C2776914184 @default.
- W2768535483 hasConcept C2778236600 @default.
- W2768535483 hasConcept C2779830541 @default.
- W2768535483 hasConcept C541997718 @default.
- W2768535483 hasConcept C55493867 @default.
- W2768535483 hasConcept C71924100 @default.
- W2768535483 hasConcept C74864618 @default.
- W2768535483 hasConcept C86803240 @default.
- W2768535483 hasConceptScore W2768535483C104317684 @default.
- W2768535483 hasConceptScore W2768535483C126322002 @default.
- W2768535483 hasConceptScore W2768535483C142724271 @default.
- W2768535483 hasConceptScore W2768535483C146249460 @default.
- W2768535483 hasConceptScore W2768535483C150903083 @default.
- W2768535483 hasConceptScore W2768535483C159654299 @default.
- W2768535483 hasConceptScore W2768535483C203014093 @default.
- W2768535483 hasConceptScore W2768535483C207001950 @default.
- W2768535483 hasConceptScore W2768535483C2776914184 @default.
- W2768535483 hasConceptScore W2768535483C2778236600 @default.
- W2768535483 hasConceptScore W2768535483C2779830541 @default.
- W2768535483 hasConceptScore W2768535483C541997718 @default.
- W2768535483 hasConceptScore W2768535483C55493867 @default.
- W2768535483 hasConceptScore W2768535483C71924100 @default.
- W2768535483 hasConceptScore W2768535483C74864618 @default.
- W2768535483 hasConceptScore W2768535483C86803240 @default.
- W2768535483 hasIssue "1" @default.
- W2768535483 hasLocation W27685354831 @default.
- W2768535483 hasLocation W27685354832 @default.
- W2768535483 hasOpenAccess W2768535483 @default.
- W2768535483 hasPrimaryLocation W27685354831 @default.
- W2768535483 hasRelatedWork W1498131314 @default.
- W2768535483 hasRelatedWork W1963972770 @default.
- W2768535483 hasRelatedWork W2022037402 @default.
- W2768535483 hasRelatedWork W2362268136 @default.
- W2768535483 hasRelatedWork W2368279279 @default.
- W2768535483 hasRelatedWork W2373402090 @default.
- W2768535483 hasRelatedWork W2438102269 @default.
- W2768535483 hasRelatedWork W3030082826 @default.
- W2768535483 hasRelatedWork W3146683103 @default.
- W2768535483 hasRelatedWork W779681777 @default.
- W2768535483 hasVolume "214" @default.
- W2768535483 isParatext "false" @default.
- W2768535483 isRetracted "false" @default.
- W2768535483 magId "2768535483" @default.
- W2768535483 workType "article" @default.