FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2769059911> ?p ?o ?g. }

• W2769059911 abstract "ABSTRACT There is a growing body of evidence suggesting that some ribonucleoside/ribonucleotide analogs may be incorporated into mitochondrial RNA by human mitochondrial DNA-dependent RNA polymerase (POLRMT) and disrupt mitochondrial RNA synthesis. An assessment of the incorporation efficiency of a ribonucleotide analog 5′-triphosphate by POLRMT may be used to evaluate the potential mitochondrial toxicity of the analog early in the development process. In this report, we provide a simple method to prepare active recombinant POLRMT. A robust in vitro nonradioactive primer extension assay was developed to assay the incorporation efficiency of ribonucleotide analog 5′-triphosphates. Our results show that many ribonucleotide analogs, including some antiviral compounds currently in various preclinical or clinical development stages, can be incorporated into newly synthesized RNA by POLRMT and that the incorporation of some of them can lead to chain termination. The discrimination ( D ) values of ribonucleotide analog 5′-triphosphates over those of natural ribonucleotide triphosphates (rNTPs) were measured to evaluate the incorporation efficiency of the ribonucleotide analog 5′-triphosphates by POLRMT. The discrimination values of natural rNTPs under the condition of misincorporation by POLRMT were used as a reference to evaluate the potential mitochondrial toxicity of ribonucleotide analogs. We propose the following criteria for the potential mitochondrial toxicity of ribonucleotide analogs based on D values: a safe compound has a D value of >10 5 ; a potentially toxic compound has a D value of >10 4 but <10 5 ; and a toxic compound has a D value of <10 4 . This report provides a simple screening method that should assist investigators in designing ribonucleoside-based drugs having lower mitochondrial toxicity." @default.
• W2769059911 created "2017-12-04" @default.
• W2769059911 creator A5014911903 @default.
• W2769059911 creator A5023177456 @default.
• W2769059911 creator A5023881228 @default.
• W2769059911 creator A5030562375 @default.
• W2769059911 creator A5031547878 @default.
• W2769059911 creator A5037864675 @default.
• W2769059911 creator A5041380813 @default.
• W2769059911 creator A5057729645 @default.
• W2769059911 creator A5069104328 @default.
• W2769059911 creator A5071558688 @default.
• W2769059911 creator A5071803277 @default.
• W2769059911 date "2018-02-01" @default.
• W2769059911 modified "2023-09-30" @default.
• W2769059911 title "Simple <i>In Vitro</i> Assay To Evaluate the Incorporation Efficiency of Ribonucleotide Analog 5′-Triphosphates into RNA by Human Mitochondrial DNA-Dependent RNA Polymerase" @default.
• W2769059911 cites W1977139658 @default.
• W2769059911 cites W1995169990 @default.
• W2769059911 cites W1999694850 @default.
• W2769059911 cites W2004917161 @default.
• W2769059911 cites W2007323926 @default.
• W2769059911 cites W2015470310 @default.
• W2769059911 cites W2031525361 @default.
• W2769059911 cites W2069284349 @default.
• W2769059911 cites W2076304130 @default.
• W2769059911 cites W2093319492 @default.
• W2769059911 cites W2120004160 @default.
• W2769059911 cites W2126985219 @default.
• W2769059911 cites W2127462625 @default.
• W2769059911 cites W2148148996 @default.
• W2769059911 cites W2153023603 @default.
• W2769059911 cites W2165960631 @default.
• W2769059911 cites W2175569396 @default.
• W2769059911 cites W2397106554 @default.
• W2769059911 cites W2410066853 @default.
• W2769059911 cites W2523163884 @default.
• W2769059911 cites W2561192435 @default.
• W2769059911 cites W2605406690 @default.
• W2769059911 cites W2618957429 @default.
• W2769059911 cites W2753473507 @default.
• W2769059911 doi "https://doi.org/10.1128/aac.01830-17" @default.
• W2769059911 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5786792" @default.
• W2769059911 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29180528" @default.
• W2769059911 hasPublicationYear "2018" @default.
• W2769059911 type Work @default.
• W2769059911 sameAs 2769059911 @default.
• W2769059911 citedByCount "8" @default.
• W2769059911 countsByYear W27690599112020 @default.
• W2769059911 countsByYear W27690599112021 @default.
• W2769059911 countsByYear W27690599112023 @default.
• W2769059911 crossrefType "journal-article" @default.
• W2769059911 hasAuthorship W2769059911A5014911903 @default.
• W2769059911 hasAuthorship W2769059911A5023177456 @default.
• W2769059911 hasAuthorship W2769059911A5023881228 @default.
• W2769059911 hasAuthorship W2769059911A5030562375 @default.
• W2769059911 hasAuthorship W2769059911A5031547878 @default.
• W2769059911 hasAuthorship W2769059911A5037864675 @default.
• W2769059911 hasAuthorship W2769059911A5041380813 @default.
• W2769059911 hasAuthorship W2769059911A5057729645 @default.
• W2769059911 hasAuthorship W2769059911A5069104328 @default.
• W2769059911 hasAuthorship W2769059911A5071558688 @default.
• W2769059911 hasAuthorship W2769059911A5071803277 @default.
• W2769059911 hasBestOaLocation W27690599111 @default.
• W2769059911 hasConcept C104317684 @default.
• W2769059911 hasConcept C153911025 @default.
• W2769059911 hasConcept C2776543447 @default.
• W2769059911 hasConcept C2776844798 @default.
• W2769059911 hasConcept C512185932 @default.
• W2769059911 hasConcept C552990157 @default.
• W2769059911 hasConcept C55493867 @default.
• W2769059911 hasConcept C67705224 @default.
• W2769059911 hasConcept C82381507 @default.
• W2769059911 hasConcept C86803240 @default.
• W2769059911 hasConcept C98892162 @default.
• W2769059911 hasConceptScore W2769059911C104317684 @default.
• W2769059911 hasConceptScore W2769059911C153911025 @default.
• W2769059911 hasConceptScore W2769059911C2776543447 @default.
• W2769059911 hasConceptScore W2769059911C2776844798 @default.
• W2769059911 hasConceptScore W2769059911C512185932 @default.
• W2769059911 hasConceptScore W2769059911C552990157 @default.
• W2769059911 hasConceptScore W2769059911C55493867 @default.
• W2769059911 hasConceptScore W2769059911C67705224 @default.
• W2769059911 hasConceptScore W2769059911C82381507 @default.
• W2769059911 hasConceptScore W2769059911C86803240 @default.
• W2769059911 hasConceptScore W2769059911C98892162 @default.
• W2769059911 hasFunder F4320310224 @default.
• W2769059911 hasIssue "2" @default.
• W2769059911 hasLocation W27690599111 @default.
• W2769059911 hasLocation W27690599112 @default.
• W2769059911 hasLocation W27690599113 @default.
• W2769059911 hasLocation W27690599114 @default.
• W2769059911 hasOpenAccess W2769059911 @default.
• W2769059911 hasPrimaryLocation W27690599111 @default.
• W2769059911 hasRelatedWork W1429146076 @default.
• W2769059911 hasRelatedWork W1976736675 @default.
• W2769059911 hasRelatedWork W2002473487 @default.
• W2769059911 hasRelatedWork W2034660554 @default.
• W2769059911 hasRelatedWork W2055085914 @default.
• W2769059911 hasRelatedWork W2474028363 @default.
• W2769059911 hasRelatedWork W26199919 @default.

• next